Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022372415> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2022372415 endingPage "179" @default.
- W2022372415 startingPage "173" @default.
- W2022372415 abstract "Necdin is a growth suppressor expressed predominantly in postmitotic neurons, and ectopic expression of this protein suppresses cell growth. Here we report that Necdin directly binds to specific DNA sequences and serves as a transcriptional repressor. Polyhistidine-tagged Necdin was used for selection of random-sequence oligonucleotides by polymerase chain reaction-based amplification. Necdin recognized guanosine (G)-rich sequences that encompass multiple G clusters and intervening mono- or di-nucleotides of A, T and C. These sequences, termed GN boxes, resemble multiply aligned forms of the canonical GC box which is recognized by Sp family members. Necdin directly bound to a GN box consisting of contiguous two GC boxes with four G clusters, but not to a single GC box with two G clusters, whereas Sp1 bound to both. In a reporter system using Drosophila Schneider Line 2 cells, Necdin repressed Sp1-dependent activity of mouse c-myc P1 promoter that contains a typical GN box. Deletion of the GN box from the c-myc P1 promoter or its conversion to the single GC box abolished the Necdin-dependent repression. These results suggest that Necdin modulates gene transcription via the GN box that is potentially recognized by GC box-targeting Sp family members." @default.
- W2022372415 created "2016-06-24" @default.
- W2022372415 creator A5000893018 @default.
- W2022372415 creator A5010385471 @default.
- W2022372415 creator A5012733495 @default.
- W2022372415 creator A5042183849 @default.
- W2022372415 date "2001-07-01" @default.
- W2022372415 modified "2023-10-18" @default.
- W2022372415 title "Necdin acts as a transcriptional repressor that interacts with multiple guanosine clusters" @default.
- W2022372415 cites W1552218246 @default.
- W2022372415 cites W1965372133 @default.
- W2022372415 cites W1966593231 @default.
- W2022372415 cites W1979216378 @default.
- W2022372415 cites W1984374449 @default.
- W2022372415 cites W1988296630 @default.
- W2022372415 cites W1999203392 @default.
- W2022372415 cites W2016730049 @default.
- W2022372415 cites W2052142601 @default.
- W2022372415 cites W2061639735 @default.
- W2022372415 cites W2076716879 @default.
- W2022372415 cites W2077313482 @default.
- W2022372415 cites W2087897472 @default.
- W2022372415 cites W2089327546 @default.
- W2022372415 cites W2099392167 @default.
- W2022372415 cites W2141942950 @default.
- W2022372415 cites W2145843666 @default.
- W2022372415 cites W2148896458 @default.
- W2022372415 doi "https://doi.org/10.1016/s0378-1119(01)00544-3" @default.
- W2022372415 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11470523" @default.
- W2022372415 hasPublicationYear "2001" @default.
- W2022372415 type Work @default.
- W2022372415 sameAs 2022372415 @default.
- W2022372415 citedByCount "35" @default.
- W2022372415 countsByYear W20223724152013 @default.
- W2022372415 countsByYear W20223724152014 @default.
- W2022372415 countsByYear W20223724152015 @default.
- W2022372415 countsByYear W20223724152016 @default.
- W2022372415 countsByYear W20223724152017 @default.
- W2022372415 countsByYear W20223724152020 @default.
- W2022372415 countsByYear W20223724152021 @default.
- W2022372415 crossrefType "journal-article" @default.
- W2022372415 hasAuthorship W2022372415A5000893018 @default.
- W2022372415 hasAuthorship W2022372415A5010385471 @default.
- W2022372415 hasAuthorship W2022372415A5012733495 @default.
- W2022372415 hasAuthorship W2022372415A5042183849 @default.
- W2022372415 hasConcept C101762097 @default.
- W2022372415 hasConcept C104317684 @default.
- W2022372415 hasConcept C138885662 @default.
- W2022372415 hasConcept C150194340 @default.
- W2022372415 hasConcept C153911025 @default.
- W2022372415 hasConcept C158448853 @default.
- W2022372415 hasConcept C179716448 @default.
- W2022372415 hasConcept C179926584 @default.
- W2022372415 hasConcept C41895202 @default.
- W2022372415 hasConcept C54355233 @default.
- W2022372415 hasConcept C552990157 @default.
- W2022372415 hasConcept C86339819 @default.
- W2022372415 hasConcept C86803240 @default.
- W2022372415 hasConceptScore W2022372415C101762097 @default.
- W2022372415 hasConceptScore W2022372415C104317684 @default.
- W2022372415 hasConceptScore W2022372415C138885662 @default.
- W2022372415 hasConceptScore W2022372415C150194340 @default.
- W2022372415 hasConceptScore W2022372415C153911025 @default.
- W2022372415 hasConceptScore W2022372415C158448853 @default.
- W2022372415 hasConceptScore W2022372415C179716448 @default.
- W2022372415 hasConceptScore W2022372415C179926584 @default.
- W2022372415 hasConceptScore W2022372415C41895202 @default.
- W2022372415 hasConceptScore W2022372415C54355233 @default.
- W2022372415 hasConceptScore W2022372415C552990157 @default.
- W2022372415 hasConceptScore W2022372415C86339819 @default.
- W2022372415 hasConceptScore W2022372415C86803240 @default.
- W2022372415 hasIssue "1-2" @default.
- W2022372415 hasLocation W20223724151 @default.
- W2022372415 hasLocation W20223724152 @default.
- W2022372415 hasOpenAccess W2022372415 @default.
- W2022372415 hasPrimaryLocation W20223724151 @default.
- W2022372415 hasRelatedWork W1685828115 @default.
- W2022372415 hasRelatedWork W1818003313 @default.
- W2022372415 hasRelatedWork W1995156097 @default.
- W2022372415 hasRelatedWork W2006952658 @default.
- W2022372415 hasRelatedWork W2011715107 @default.
- W2022372415 hasRelatedWork W2039568244 @default.
- W2022372415 hasRelatedWork W2088889402 @default.
- W2022372415 hasRelatedWork W2121777152 @default.
- W2022372415 hasRelatedWork W2392370788 @default.
- W2022372415 hasRelatedWork W2472928735 @default.
- W2022372415 hasVolume "272" @default.
- W2022372415 isParatext "false" @default.
- W2022372415 isRetracted "false" @default.
- W2022372415 magId "2022372415" @default.
- W2022372415 workType "article" @default.