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• W2022374944 endingPage "729" @default.
• W2022374944 startingPage "720" @default.
• W2022374944 abstract "The signal recognition particle 54-kDa subunit (SRP54) binds to the signal sequences of nascent presecretory and transmembrane proteins. Previous studies have shown that signal sequences bind to the C-terminal methionine-rich domain of the protein (M-domain), but have raised the possibility that either the N-terminal domain (N-domain) or the central guanosine triphosphatase module (GTPase-domain) also contribute to signal-sequence-binding activity. We have generated a series of N-domain and GTPase-domain mutants to investigate this issue further. Mutations in a conserved N-domain motif (ALLEADV) produced significant defects in signal sequence binding that correlate with the severity of the mutation. The magnitude of the defect was independent of the preprotein substrate, which suggested that the mutations do not alter the specificity of signal sequence recognition. The N-domain mutants also showed defects in promoting the translocation of presecretory proteins across the membrane of microsomal vesicles, but these defects appeared to be a direct consequence of the reduction in signal-sequence-binding activity and not separate effects of the mutations. By contrast, mutations in the guanosine triphosphatase consensus sequence had no effect on signal sequence binding, but instead severely impaired protein translocation activity. These results indicate that a principal function of the SRP54 N-domain is to promote efficient signal sequence binding. These data also suggest that the SRP54 GTPase regulates the cycle of signal sequence binding and release, perhaps by modulating the relative orientation of the N- and M-domains." @default.
• W2022374944 created "2016-06-24" @default.
• W2022374944 creator A5059226795 @default.
• W2022374944 creator A5062522223 @default.
• W2022374944 date "1997-05-01" @default.
• W2022374944 modified "2023-09-24" @default.
• W2022374944 title "The N-Domain of the Signal Recognition Particle 54-kDa Subunit Promotes Efficient Signal Sequence Binding" @default.
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• W2022374944 doi "https://doi.org/10.1111/j.1432-1033.1997.00720.x" @default.
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