FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022377170> ?p ?o ?g. }

• W2022377170 endingPage "410" @default.
• W2022377170 startingPage "402" @default.
• W2022377170 abstract "RNA-binding proteins of coronavirus MHV-A59 were identified using an RNA overlay-protein blot assay (ROPBA). The major viral RNA-binding protein in virions and infected cells was the phosphorylated nucleocapsid protein N (50K). A new 140K virus structural protein was identified as a minor RNA-binding protein both in virions and in infected cells. The 140K protein was antigenically related to N, and upon reduction, yielded only 50K N. Thus, the 140K protein is probably a trimer of N subunits linked by intermolecular disulfide bonds. Several cellular RNA-binding proteins were also detected. RNA-binding of N was not nucleotide sequence specific. Single-stranded RNA of MHV, VSV, or cellular origin, a DNA probe of the MHV leader sequence, and double-stranded bovine rotavirus RNA could all bind to N. Binding of MHV RNA was optimal between pH 7 and 8, and the RNA could be eluted in 0.1 M NaCl. The ROPBA is a useful method for the initial identification of RNA-binding proteins, such as N and the 140K protein of murine coronavirus." @default.
• W2022377170 created "2016-06-24" @default.
• W2022377170 creator A5000438275 @default.
• W2022377170 creator A5027121399 @default.
• W2022377170 creator A5047446947 @default.
• W2022377170 creator A5053488262 @default.
• W2022377170 creator A5073264142 @default.
• W2022377170 date "1986-04-01" @default.
• W2022377170 modified "2023-10-03" @default.
• W2022377170 title "RNA-binding proteins of coronavirus MHV: Detection of monomeric and multimeric N protein with an RNA overlay-protein blot assay" @default.
• W2022377170 cites W1518231959 @default.
• W2022377170 cites W1528925470 @default.
• W2022377170 cites W1529440054 @default.
• W2022377170 cites W1557962201 @default.
• W2022377170 cites W1573008071 @default.
• W2022377170 cites W1573309488 @default.
• W2022377170 cites W1592633150 @default.
• W2022377170 cites W1723090750 @default.
• W2022377170 cites W1775749144 @default.
• W2022377170 cites W1867432506 @default.
• W2022377170 cites W1891768184 @default.
• W2022377170 cites W1964331418 @default.
• W2022377170 cites W1964585388 @default.
• W2022377170 cites W1987176584 @default.
• W2022377170 cites W2008148453 @default.
• W2022377170 cites W2039793235 @default.
• W2022377170 cites W2039918999 @default.
• W2022377170 cites W2052705327 @default.
• W2022377170 cites W2053433915 @default.
• W2022377170 cites W2053654911 @default.
• W2022377170 cites W2059202803 @default.
• W2022377170 cites W2065276348 @default.
• W2022377170 cites W2066836230 @default.
• W2022377170 cites W2074487411 @default.
• W2022377170 cites W2077349154 @default.
• W2022377170 cites W2087062084 @default.
• W2022377170 cites W2098919352 @default.
• W2022377170 cites W2100837269 @default.
• W2022377170 cites W2107898006 @default.
• W2022377170 cites W2109707787 @default.
• W2022377170 cites W2116807710 @default.
• W2022377170 cites W2126915562 @default.
• W2022377170 cites W2137186789 @default.
• W2022377170 cites W2142447649 @default.
• W2022377170 cites W2145397796 @default.
• W2022377170 cites W2153040986 @default.
• W2022377170 cites W2166408848 @default.
• W2022377170 cites W30684064 @default.
• W2022377170 cites W4293247451 @default.
• W2022377170 doi "https://doi.org/10.1016/0042-6822(86)90305-3" @default.
• W2022377170 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7131793" @default.
• W2022377170 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3083580" @default.
• W2022377170 hasPublicationYear "1986" @default.
• W2022377170 type Work @default.
• W2022377170 sameAs 2022377170 @default.
• W2022377170 citedByCount "81" @default.
• W2022377170 countsByYear W20223771702012 @default.
• W2022377170 countsByYear W20223771702014 @default.
• W2022377170 countsByYear W20223771702015 @default.
• W2022377170 countsByYear W20223771702016 @default.
• W2022377170 countsByYear W20223771702019 @default.
• W2022377170 countsByYear W20223771702020 @default.
• W2022377170 countsByYear W20223771702021 @default.
• W2022377170 countsByYear W20223771702022 @default.
• W2022377170 countsByYear W20223771702023 @default.
• W2022377170 crossrefType "journal-article" @default.
• W2022377170 hasAuthorship W2022377170A5000438275 @default.
• W2022377170 hasAuthorship W2022377170A5027121399 @default.
• W2022377170 hasAuthorship W2022377170A5047446947 @default.
• W2022377170 hasAuthorship W2022377170A5053488262 @default.
• W2022377170 hasAuthorship W2022377170A5073264142 @default.
• W2022377170 hasBestOaLocation W20223771701 @default.
• W2022377170 hasConcept C104317684 @default.
• W2022377170 hasConcept C142724271 @default.
• W2022377170 hasConcept C153911025 @default.
• W2022377170 hasConcept C2777648638 @default.
• W2022377170 hasConcept C2779134260 @default.
• W2022377170 hasConcept C3008058167 @default.
• W2022377170 hasConcept C41258723 @default.
• W2022377170 hasConcept C41282012 @default.
• W2022377170 hasConcept C524204448 @default.
• W2022377170 hasConcept C55493867 @default.
• W2022377170 hasConcept C67705224 @default.
• W2022377170 hasConcept C71924100 @default.
• W2022377170 hasConcept C86803240 @default.
• W2022377170 hasConceptScore W2022377170C104317684 @default.
• W2022377170 hasConceptScore W2022377170C142724271 @default.
• W2022377170 hasConceptScore W2022377170C153911025 @default.
• W2022377170 hasConceptScore W2022377170C2777648638 @default.
• W2022377170 hasConceptScore W2022377170C2779134260 @default.
• W2022377170 hasConceptScore W2022377170C3008058167 @default.
• W2022377170 hasConceptScore W2022377170C41258723 @default.
• W2022377170 hasConceptScore W2022377170C41282012 @default.
• W2022377170 hasConceptScore W2022377170C524204448 @default.
• W2022377170 hasConceptScore W2022377170C55493867 @default.
• W2022377170 hasConceptScore W2022377170C67705224 @default.
• W2022377170 hasConceptScore W2022377170C71924100 @default.
• W2022377170 hasConceptScore W2022377170C86803240 @default.

• next