FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022379417> ?p ?o ?g. }

• W2022379417 endingPage "366" @default.
• W2022379417 startingPage "361" @default.
• W2022379417 abstract "Our previous studies show that 10 xanthine oxidase (XO) variants (Arg149Cys, Pro555Ser, Arg607Gln, Thr623Ile, Ile703Val Asn909Lys, Thr910Lys, Pro1150Arg, His1221Arg, and Cys1318Tyr) exhibit altered activity toward the endogenous substrate xanthine. This study investigates whether these variants also exhibit altered kinetics for the exogenous substrate 6-thioxanthine (6-TX). To investigate the kinetics of wild-type XO and these variants, expression constructs were transfected into mammalian COS-7 cells. S-9 fractions containing the expressed proteins were used to determine their kinetic parameters, i.e., Km, Vmax, and intrinsic clearance (CLint), for the substrate 6-TX. Functional characterization of the 10 XO variants revealed that 4 of the variants (Arg149Cys, Asn909Lys, Thr910Lys, and Pro1150Arg) were inactive, 2 (Arg607Gln, and Cys1318Tyr) had reduced activity (CLint, 55.5% and 64.7% less than that of wild-type XO, respectively). This study provides comprehensive data regarding how genetic variation in XO affects its activity toward 6-TX. We found that the in vitro activity of 8 of the XO variants toward 6-TX was functionally affected. These results suggeste that polymorphism in the gene encoding XO may increase the toxicity of thiopurine drugs such as 6-mercaputopurine." @default.
• W2022379417 created "2016-06-24" @default.
• W2022379417 creator A5012209368 @default.
• W2022379417 creator A5023757160 @default.
• W2022379417 creator A5052165087 @default.
• W2022379417 creator A5090833783 @default.
• W2022379417 creator A5090905175 @default.
• W2022379417 date "2010-01-01" @default.
• W2022379417 modified "2023-09-26" @default.
• W2022379417 title "Kinetics of 6-Thioxanthine Metabolism by Allelic Variants of Xanthine Oxidase" @default.
• W2022379417 cites W1256525631 @default.
• W2022379417 cites W141364591 @default.
• W2022379417 cites W1828830410 @default.
• W2022379417 cites W1952461139 @default.
• W2022379417 cites W1964947822 @default.
• W2022379417 cites W1967490229 @default.
• W2022379417 cites W1967497251 @default.
• W2022379417 cites W1967512152 @default.
• W2022379417 cites W1976697895 @default.
• W2022379417 cites W1980165182 @default.
• W2022379417 cites W1984326321 @default.
• W2022379417 cites W1985827615 @default.
• W2022379417 cites W1988419817 @default.
• W2022379417 cites W2015379175 @default.
• W2022379417 cites W2023648193 @default.
• W2022379417 cites W2024758091 @default.
• W2022379417 cites W2031561085 @default.
• W2022379417 cites W2037125325 @default.
• W2022379417 cites W2047522682 @default.
• W2022379417 cites W2054045668 @default.
• W2022379417 cites W2054782645 @default.
• W2022379417 cites W2079357945 @default.
• W2022379417 cites W2084802783 @default.
• W2022379417 cites W2086196338 @default.
• W2022379417 cites W2132517981 @default.
• W2022379417 cites W2133228225 @default.
• W2022379417 cites W2139458933 @default.
• W2022379417 cites W2167939965 @default.
• W2022379417 cites W2413837558 @default.
• W2022379417 cites W4324120057 @default.
• W2022379417 doi "https://doi.org/10.2133/dmpk.dmpk-10-rg-029" @default.
• W2022379417 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20814157" @default.
• W2022379417 hasPublicationYear "2010" @default.
• W2022379417 type Work @default.
• W2022379417 sameAs 2022379417 @default.
• W2022379417 citedByCount "11" @default.
• W2022379417 countsByYear W20223794172012 @default.
• W2022379417 countsByYear W20223794172013 @default.
• W2022379417 countsByYear W20223794172015 @default.
• W2022379417 countsByYear W20223794172016 @default.
• W2022379417 countsByYear W20223794172021 @default.
• W2022379417 crossrefType "journal-article" @default.
• W2022379417 hasAuthorship W2022379417A5012209368 @default.
• W2022379417 hasAuthorship W2022379417A5023757160 @default.
• W2022379417 hasAuthorship W2022379417A5052165087 @default.
• W2022379417 hasAuthorship W2022379417A5090833783 @default.
• W2022379417 hasAuthorship W2022379417A5090905175 @default.
• W2022379417 hasConcept C104317684 @default.
• W2022379417 hasConcept C121332964 @default.
• W2022379417 hasConcept C148898269 @default.
• W2022379417 hasConcept C151400659 @default.
• W2022379417 hasConcept C153911025 @default.
• W2022379417 hasConcept C180754005 @default.
• W2022379417 hasConcept C181199279 @default.
• W2022379417 hasConcept C185592680 @default.
• W2022379417 hasConcept C18903297 @default.
• W2022379417 hasConcept C202751555 @default.
• W2022379417 hasConcept C2777289219 @default.
• W2022379417 hasConcept C2778102346 @default.
• W2022379417 hasConcept C2779470414 @default.
• W2022379417 hasConcept C55493867 @default.
• W2022379417 hasConcept C62231903 @default.
• W2022379417 hasConcept C62520636 @default.
• W2022379417 hasConcept C86803240 @default.
• W2022379417 hasConceptScore W2022379417C104317684 @default.
• W2022379417 hasConceptScore W2022379417C121332964 @default.
• W2022379417 hasConceptScore W2022379417C148898269 @default.
• W2022379417 hasConceptScore W2022379417C151400659 @default.
• W2022379417 hasConceptScore W2022379417C153911025 @default.
• W2022379417 hasConceptScore W2022379417C180754005 @default.
• W2022379417 hasConceptScore W2022379417C181199279 @default.
• W2022379417 hasConceptScore W2022379417C185592680 @default.
• W2022379417 hasConceptScore W2022379417C18903297 @default.
• W2022379417 hasConceptScore W2022379417C202751555 @default.
• W2022379417 hasConceptScore W2022379417C2777289219 @default.
• W2022379417 hasConceptScore W2022379417C2778102346 @default.
• W2022379417 hasConceptScore W2022379417C2779470414 @default.
• W2022379417 hasConceptScore W2022379417C55493867 @default.
• W2022379417 hasConceptScore W2022379417C62231903 @default.
• W2022379417 hasConceptScore W2022379417C62520636 @default.
• W2022379417 hasConceptScore W2022379417C86803240 @default.
• W2022379417 hasIssue "4" @default.
• W2022379417 hasLocation W20223794171 @default.
• W2022379417 hasLocation W20223794172 @default.
• W2022379417 hasOpenAccess W2022379417 @default.
• W2022379417 hasPrimaryLocation W20223794171 @default.
• W2022379417 hasRelatedWork W1753158170 @default.
• W2022379417 hasRelatedWork W1978283601 @default.

• next