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- W2022383871 abstract "Abstract Acridine orange direct count (AODC) is a popular method for determining microbial numbers in environmental samples via epifluorescence microscopy. We have found that the color of the fluorescing cells (green vs orange-red cells) depends on the level of moisture on the filter and that the percentage of green-fluorescing cells decreases when the filter is dry. Since the level of moisture can vary across the filter and is difficult to control in any case, the use of color as a basis for inferring cell activity is not justified. The proportion of cells fluorescing green on a previously dried filter can be greatly increased by remoistening the filter prior to microscopic examination. This is advantageous since cells which fluoresce green are more easily visualized than orange-red fluorescing cells. We therefore propose modification of the Standard Methods protocol for AODC to include the remoistening step." @default.
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- W2022383871 date "1993-07-01" @default.
- W2022383871 modified "2023-09-26" @default.
- W2022383871 title "Modification of the standard epifluorescence microscopic method for total bacterial counts in environmental samples" @default.
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- W2022383871 doi "https://doi.org/10.1016/0043-1354(93)90001-x" @default.
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