FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022384406> ?p ?o ?g. }

• W2022384406 endingPage "854" @default.
• W2022384406 startingPage "842" @default.
• W2022384406 abstract "The in vivo interaction of estrogen receptor (ER) and Hsp90, demonstrated in the absence of hormone by a nuclear cotranslocation assay of the cytoplasmic Hsp90 with the karyophilic receptor, was disrupted by agonist and antagonist ligands, which, after dissociating the Hsp90, allowed the chaperone protein to be relocalized in the cytoplasm. The pure antiestrogen RU 58668 (RU), which was unable to stimulate an estrogen-dependent reporter gene and completely inhibited its estradiol-induced activity, also profoundly modified the subcellular distribution of ER in a specific time- and dose-dependent manner; ER appeared as speckled fluorescent clusters mainly located in the perinuclear region of the cytoplasm. The kinetics of appearance and reversal of the RU-dependent ER mislocalization in the presence or absence of cycloheximide demonstrated 1) that this effect was reversed by RU withdrawal or estradiol (E2) treatment, and 2) that cycloheximide with RU inhibited and reversed the ER cytoplasmic mislocalization induced by RU alone. These results point to a protein synthesis-dependent step in the mechanism of action of this antiestrogen. After RU treatment, a large portion of ER was found in the particulate fraction of the cytoplasm. However, confocal and electron microscopic analysis showed that ER clusters were not associated with specific cytoplasmic organelles or compartments. Using ER mutants, it was found that the ligand binding domain was sufficient for RU to produce receptor mislocalization, while the constitutive nuclear localization signals were dispensable. We propose that the antiestrogenic properties of RU are primarily due to the induction of an aggregation-prone receptor conformation that cannot undertake the constitutive and the ligand-induced nuclear localization function of the receptor because it is sequestered in the cytoplasm by fast turning over protein(s). We predict that antiestrogens able to block ER nuclear localization will behave as pure antihormones and will inhibit all the nuclear action of ER elicited by agonistic ligands or by ligand-independent mechanisms such as growth factor stimulation." @default.
• W2022384406 created "2016-06-24" @default.
• W2022384406 creator A5001677651 @default.
• W2022384406 creator A5002615106 @default.
• W2022384406 creator A5042539740 @default.
• W2022384406 creator A5069049455 @default.
• W2022384406 creator A5070917474 @default.
• W2022384406 creator A5090399312 @default.
• W2022384406 date "1998-06-01" @default.
• W2022384406 modified "2023-10-18" @default.
• W2022384406 title "Interaction and Dissociation by Ligands of Estrogen Receptor and Hsp90: The Antiestrogen RU 58668 Induces a Protein Synthesis-Dependent Clustering of the Receptor in the Cytoplasm" @default.
• W2022384406 cites W1493268813 @default.
• W2022384406 cites W1494393764 @default.
• W2022384406 cites W1944448503 @default.
• W2022384406 cites W1956379070 @default.
• W2022384406 cites W1971861967 @default.
• W2022384406 cites W1974357996 @default.
• W2022384406 cites W1976495127 @default.
• W2022384406 cites W1978754402 @default.
• W2022384406 cites W1983151017 @default.
• W2022384406 cites W1987183940 @default.
• W2022384406 cites W1999985166 @default.
• W2022384406 cites W2004376767 @default.
• W2022384406 cites W2009975411 @default.
• W2022384406 cites W2010142538 @default.
• W2022384406 cites W2014817101 @default.
• W2022384406 cites W2019310947 @default.
• W2022384406 cites W2030762899 @default.
• W2022384406 cites W2036360644 @default.
• W2022384406 cites W2040053707 @default.
• W2022384406 cites W2043881813 @default.
• W2022384406 cites W2052059014 @default.
• W2022384406 cites W2057700119 @default.
• W2022384406 cites W2058649944 @default.
• W2022384406 cites W2059105996 @default.
• W2022384406 cites W2065865631 @default.
• W2022384406 cites W2066441904 @default.
• W2022384406 cites W2068733105 @default.
• W2022384406 cites W2068733538 @default.
• W2022384406 cites W2084639732 @default.
• W2022384406 cites W2101738855 @default.
• W2022384406 cites W2128840922 @default.
• W2022384406 cites W2129289858 @default.
• W2022384406 cites W2142365365 @default.
• W2022384406 cites W2147089981 @default.
• W2022384406 cites W2147673480 @default.
• W2022384406 cites W2148307287 @default.
• W2022384406 cites W2154762345 @default.
• W2022384406 cites W2158140522 @default.
• W2022384406 cites W2160402926 @default.
• W2022384406 cites W2172256092 @default.
• W2022384406 cites W2177151394 @default.
• W2022384406 cites W2399914984 @default.
• W2022384406 cites W2401253719 @default.
• W2022384406 cites W270887500 @default.
• W2022384406 cites W69516432 @default.
• W2022384406 doi "https://doi.org/10.1210/mend.12.6.0121" @default.
• W2022384406 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9626660" @default.
• W2022384406 hasPublicationYear "1998" @default.
• W2022384406 type Work @default.
• W2022384406 sameAs 2022384406 @default.
• W2022384406 citedByCount "58" @default.
• W2022384406 countsByYear W20223844062012 @default.
• W2022384406 countsByYear W20223844062015 @default.
• W2022384406 countsByYear W20223844062016 @default.
• W2022384406 countsByYear W20223844062018 @default.
• W2022384406 countsByYear W20223844062020 @default.
• W2022384406 countsByYear W20223844062021 @default.
• W2022384406 crossrefType "journal-article" @default.
• W2022384406 hasAuthorship W2022384406A5001677651 @default.
• W2022384406 hasAuthorship W2022384406A5002615106 @default.
• W2022384406 hasAuthorship W2022384406A5042539740 @default.
• W2022384406 hasAuthorship W2022384406A5069049455 @default.
• W2022384406 hasAuthorship W2022384406A5070917474 @default.
• W2022384406 hasAuthorship W2022384406A5090399312 @default.
• W2022384406 hasBestOaLocation W20223844061 @default.
• W2022384406 hasConcept C104317684 @default.
• W2022384406 hasConcept C121608353 @default.
• W2022384406 hasConcept C142724271 @default.
• W2022384406 hasConcept C153911025 @default.
• W2022384406 hasConcept C170493617 @default.
• W2022384406 hasConcept C190062978 @default.
• W2022384406 hasConcept C205260736 @default.
• W2022384406 hasConcept C2775932338 @default.
• W2022384406 hasConcept C2775962898 @default.
• W2022384406 hasConcept C2776067312 @default.
• W2022384406 hasConcept C2778128677 @default.
• W2022384406 hasConcept C3675279 @default.
• W2022384406 hasConcept C530470458 @default.
• W2022384406 hasConcept C54355233 @default.
• W2022384406 hasConcept C55493867 @default.
• W2022384406 hasConcept C71924100 @default.
• W2022384406 hasConcept C84606932 @default.
• W2022384406 hasConcept C86803240 @default.
• W2022384406 hasConcept C95444343 @default.
• W2022384406 hasConceptScore W2022384406C104317684 @default.
• W2022384406 hasConceptScore W2022384406C121608353 @default.
• W2022384406 hasConceptScore W2022384406C142724271 @default.

• next