FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022398889> ?p ?o ?g. }

• W2022398889 endingPage "762" @default.
• W2022398889 startingPage "758" @default.
• W2022398889 abstract "The essence of the Drosophila circadian clock involves an autoregulatory feedback loop in which PERIOD (PER) and TIMELESS (TIM) inhibit their own transcription by association with the transcriptional activators dCLOCK (dCLK) and CYCLE (CYC). Because PER, dCLK, and CYC each contain a PAS domain, it has been assumed that these interaction domains are important for negative feedback. However, a critical role for PAS-PAS interactions in Drosophila clock function has not been shown. Nuclear transport of PER is also believed to be an essential regulatory step for negative feedback, but this has not been directly tested, and the relevant nuclear localization sequence (NLS) has not been functionally mapped. We evaluated these critical aspects of PER-mediated transcriptional inhibition in Drosophila Schneider 2 (S2) cells. We mapped the dCLK:CYC inhibition domain (CCID) of PER and discovered that it lies in the C terminus, downstream of the PAS domain. Using deletion mutants and site-directed mutagenesis, we identified a novel NLS in the CCID of PER that is a potent regulator of PER's nuclear transport in S2 cells. We further found that nuclear transport, primarily through this novel NLS, is essential for the inhibitory activity of PER. The data indicate that nuclear PER inhibits dCLK:CYC-mediated transcription through a novel domain that additionally contains a potent NLS." @default.
• W2022398889 created "2016-06-24" @default.
• W2022398889 creator A5008739782 @default.
• W2022398889 creator A5010565220 @default.
• W2022398889 date "2003-04-01" @default.
• W2022398889 modified "2023-10-01" @default.
• W2022398889 title "A Novel C-Terminal Domain of Drosophila PERIOD Inhibits dCLOCK:CYCLE-Mediated Transcription" @default.
• W2022398889 cites W1818413434 @default.
• W2022398889 cites W1968410906 @default.
• W2022398889 cites W1995139743 @default.
• W2022398889 cites W1996454474 @default.
• W2022398889 cites W2023325911 @default.
• W2022398889 cites W2030063045 @default.
• W2022398889 cites W2040561723 @default.
• W2022398889 cites W2059288996 @default.
• W2022398889 cites W2065793682 @default.
• W2022398889 cites W2085853202 @default.
• W2022398889 cites W2128443000 @default.
• W2022398889 cites W2140721783 @default.
• W2022398889 cites W2148181747 @default.
• W2022398889 cites W2149035004 @default.
• W2022398889 cites W2165306451 @default.
• W2022398889 cites W2168037738 @default.
• W2022398889 doi "https://doi.org/10.1016/s0960-9822(03)00286-0" @default.
• W2022398889 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12725734" @default.
• W2022398889 hasPublicationYear "2003" @default.
• W2022398889 type Work @default.
• W2022398889 sameAs 2022398889 @default.
• W2022398889 citedByCount "108" @default.
• W2022398889 countsByYear W20223988892012 @default.
• W2022398889 countsByYear W20223988892013 @default.
• W2022398889 countsByYear W20223988892014 @default.
• W2022398889 countsByYear W20223988892015 @default.
• W2022398889 countsByYear W20223988892016 @default.
• W2022398889 countsByYear W20223988892017 @default.
• W2022398889 countsByYear W20223988892018 @default.
• W2022398889 countsByYear W20223988892019 @default.
• W2022398889 countsByYear W20223988892020 @default.
• W2022398889 countsByYear W20223988892021 @default.
• W2022398889 countsByYear W20223988892022 @default.
• W2022398889 countsByYear W20223988892023 @default.
• W2022398889 crossrefType "journal-article" @default.
• W2022398889 hasAuthorship W2022398889A5008739782 @default.
• W2022398889 hasAuthorship W2022398889A5010565220 @default.
• W2022398889 hasBestOaLocation W20223988891 @default.
• W2022398889 hasConcept C104317684 @default.
• W2022398889 hasConcept C121332964 @default.
• W2022398889 hasConcept C121446783 @default.
• W2022398889 hasConcept C138885662 @default.
• W2022398889 hasConcept C143065580 @default.
• W2022398889 hasConcept C169760540 @default.
• W2022398889 hasConcept C179926584 @default.
• W2022398889 hasConcept C186435350 @default.
• W2022398889 hasConcept C24890656 @default.
• W2022398889 hasConcept C2778875234 @default.
• W2022398889 hasConcept C2778880379 @default.
• W2022398889 hasConcept C2780114586 @default.
• W2022398889 hasConcept C2780723820 @default.
• W2022398889 hasConcept C2781291010 @default.
• W2022398889 hasConcept C38606739 @default.
• W2022398889 hasConcept C41895202 @default.
• W2022398889 hasConcept C49805395 @default.
• W2022398889 hasConcept C54355233 @default.
• W2022398889 hasConcept C59157529 @default.
• W2022398889 hasConcept C86339819 @default.
• W2022398889 hasConcept C86803240 @default.
• W2022398889 hasConcept C95444343 @default.
• W2022398889 hasConceptScore W2022398889C104317684 @default.
• W2022398889 hasConceptScore W2022398889C121332964 @default.
• W2022398889 hasConceptScore W2022398889C121446783 @default.
• W2022398889 hasConceptScore W2022398889C138885662 @default.
• W2022398889 hasConceptScore W2022398889C143065580 @default.
• W2022398889 hasConceptScore W2022398889C169760540 @default.
• W2022398889 hasConceptScore W2022398889C179926584 @default.
• W2022398889 hasConceptScore W2022398889C186435350 @default.
• W2022398889 hasConceptScore W2022398889C24890656 @default.
• W2022398889 hasConceptScore W2022398889C2778875234 @default.
• W2022398889 hasConceptScore W2022398889C2778880379 @default.
• W2022398889 hasConceptScore W2022398889C2780114586 @default.
• W2022398889 hasConceptScore W2022398889C2780723820 @default.
• W2022398889 hasConceptScore W2022398889C2781291010 @default.
• W2022398889 hasConceptScore W2022398889C38606739 @default.
• W2022398889 hasConceptScore W2022398889C41895202 @default.
• W2022398889 hasConceptScore W2022398889C49805395 @default.
• W2022398889 hasConceptScore W2022398889C54355233 @default.
• W2022398889 hasConceptScore W2022398889C59157529 @default.
• W2022398889 hasConceptScore W2022398889C86339819 @default.
• W2022398889 hasConceptScore W2022398889C86803240 @default.
• W2022398889 hasConceptScore W2022398889C95444343 @default.
• W2022398889 hasIssue "9" @default.
• W2022398889 hasLocation W20223988891 @default.
• W2022398889 hasLocation W20223988892 @default.
• W2022398889 hasOpenAccess W2022398889 @default.
• W2022398889 hasPrimaryLocation W20223988891 @default.
• W2022398889 hasRelatedWork W1622989995 @default.
• W2022398889 hasRelatedWork W1993536416 @default.
• W2022398889 hasRelatedWork W2008897992 @default.
• W2022398889 hasRelatedWork W2011897894 @default.

• next