FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022425145> ?p ?o ?g. }

• W2022425145 endingPage "165" @default.
• W2022425145 startingPage "158" @default.
• W2022425145 abstract "We report an innovative at-line method to monitor concentration of bioactive antibody (i.e., antibody with conserved antigen-binding activity) secreted during bioreactor culture by the use of surface plasmon resonance (SPR)-based biosensor technology. In a first series of experiments, conditions for SPR-based measurements were validated off-line by monitoring bioactive antibody concentration in conditioned medium from 500-ml baffled flask hybridoma cell cultures. A fully automated experimental setup in which the SPR-based biosensor was harnessed to a bioreactor was then used at-line to monitor the concentration of bioactive antibody produced in a 3.5-L bioreactor. Quantitative SPR measurements performed both at-line and off-line were in excellent agreement with quantitative Western blotting followed by densitometry analyses. Thus, our experimental study confirms that SPR biosensors can be applied to at-line quantification of correctly folded proteins that are secreted by cells cultured in a bioreactor. Our experimental approach represents a novel and robust analytical strategy to be applied to the control and optimization of the production of bioactive secreted proteins." @default.
• W2022425145 created "2016-06-24" @default.
• W2022425145 creator A5007524667 @default.
• W2022425145 creator A5009718888 @default.
• W2022425145 creator A5018393891 @default.
• W2022425145 creator A5038272339 @default.
• W2022425145 creator A5051792352 @default.
• W2022425145 date "2008-07-01" @default.
• W2022425145 modified "2023-09-27" @default.
• W2022425145 title "At-line quantification of bioactive antibody in bioreactor by surface plasmon resonance using epitope detection" @default.
• W2022425145 cites W1965340481 @default.
• W2022425145 cites W1970545446 @default.
• W2022425145 cites W1986376155 @default.
• W2022425145 cites W1989963169 @default.
• W2022425145 cites W1990235837 @default.
• W2022425145 cites W2000565132 @default.
• W2022425145 cites W2028284841 @default.
• W2022425145 cites W2036753716 @default.
• W2022425145 cites W2053713849 @default.
• W2022425145 cites W2056368762 @default.
• W2022425145 cites W2061496234 @default.
• W2022425145 cites W2083706383 @default.
• W2022425145 cites W2085760943 @default.
• W2022425145 cites W2096927841 @default.
• W2022425145 cites W2098983712 @default.
• W2022425145 cites W2122362009 @default.
• W2022425145 cites W2126308493 @default.
• W2022425145 cites W2136885756 @default.
• W2022425145 cites W4233249586 @default.
• W2022425145 cites W2071175437 @default.
• W2022425145 doi "https://doi.org/10.1016/j.ab.2008.04.019" @default.
• W2022425145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18457651" @default.
• W2022425145 hasPublicationYear "2008" @default.
• W2022425145 type Work @default.
• W2022425145 sameAs 2022425145 @default.
• W2022425145 citedByCount "31" @default.
• W2022425145 countsByYear W20224251452012 @default.
• W2022425145 countsByYear W20224251452013 @default.
• W2022425145 countsByYear W20224251452014 @default.
• W2022425145 countsByYear W20224251452015 @default.
• W2022425145 countsByYear W20224251452016 @default.
• W2022425145 countsByYear W20224251452017 @default.
• W2022425145 countsByYear W20224251452019 @default.
• W2022425145 countsByYear W20224251452020 @default.
• W2022425145 countsByYear W20224251452021 @default.
• W2022425145 countsByYear W20224251452022 @default.
• W2022425145 crossrefType "journal-article" @default.
• W2022425145 hasAuthorship W2022425145A5007524667 @default.
• W2022425145 hasAuthorship W2022425145A5009718888 @default.
• W2022425145 hasAuthorship W2022425145A5018393891 @default.
• W2022425145 hasAuthorship W2022425145A5038272339 @default.
• W2022425145 hasAuthorship W2022425145A5051792352 @default.
• W2022425145 hasConcept C104317684 @default.
• W2022425145 hasConcept C106847996 @default.
• W2022425145 hasConcept C120665830 @default.
• W2022425145 hasConcept C121332964 @default.
• W2022425145 hasConcept C136496925 @default.
• W2022425145 hasConcept C155672457 @default.
• W2022425145 hasConcept C159654299 @default.
• W2022425145 hasConcept C160756335 @default.
• W2022425145 hasConcept C168170006 @default.
• W2022425145 hasConcept C171250308 @default.
• W2022425145 hasConcept C178790620 @default.
• W2022425145 hasConcept C185592680 @default.
• W2022425145 hasConcept C192562407 @default.
• W2022425145 hasConcept C203014093 @default.
• W2022425145 hasConcept C43617362 @default.
• W2022425145 hasConcept C55493867 @default.
• W2022425145 hasConcept C85265743 @default.
• W2022425145 hasConcept C86803240 @default.
• W2022425145 hasConceptScore W2022425145C104317684 @default.
• W2022425145 hasConceptScore W2022425145C106847996 @default.
• W2022425145 hasConceptScore W2022425145C120665830 @default.
• W2022425145 hasConceptScore W2022425145C121332964 @default.
• W2022425145 hasConceptScore W2022425145C136496925 @default.
• W2022425145 hasConceptScore W2022425145C155672457 @default.
• W2022425145 hasConceptScore W2022425145C159654299 @default.
• W2022425145 hasConceptScore W2022425145C160756335 @default.
• W2022425145 hasConceptScore W2022425145C168170006 @default.
• W2022425145 hasConceptScore W2022425145C171250308 @default.
• W2022425145 hasConceptScore W2022425145C178790620 @default.
• W2022425145 hasConceptScore W2022425145C185592680 @default.
• W2022425145 hasConceptScore W2022425145C192562407 @default.
• W2022425145 hasConceptScore W2022425145C203014093 @default.
• W2022425145 hasConceptScore W2022425145C43617362 @default.
• W2022425145 hasConceptScore W2022425145C55493867 @default.
• W2022425145 hasConceptScore W2022425145C85265743 @default.
• W2022425145 hasConceptScore W2022425145C86803240 @default.
• W2022425145 hasIssue "2" @default.
• W2022425145 hasLocation W20224251451 @default.
• W2022425145 hasLocation W20224251452 @default.
• W2022425145 hasOpenAccess W2022425145 @default.
• W2022425145 hasPrimaryLocation W20224251451 @default.
• W2022425145 hasRelatedWork W1967701792 @default.
• W2022425145 hasRelatedWork W2022425145 @default.
• W2022425145 hasRelatedWork W2030398297 @default.
• W2022425145 hasRelatedWork W2043486114 @default.
• W2022425145 hasRelatedWork W2044499942 @default.

• next