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- W2022436558 abstract "Suspension cultures of six Lolium perenne varieties were initiated from immature inflorescence-derived compact callus or from seed-derived embryos. Established suspension cultures were used for the isolation of protoplasts. Protoplasts were cultured in liquid as well as in agarose-solidified medium. Finely dispersed embryogenic suspension cultures with a capacity to regenerate green plants were obtained from five L. perenne varieties. Relatively more suspension cultures became established when immature inflorescence-derived callus was used for initiation. With few exceptions, the capacity to regenerate green shoots decreased with suspension culture age and was totally lost on average 25 weeks after suspension culture initiation. Fast-growing, non-morphogenic suspension cultures yielded large numbers of protoplasts that formed numerous microcalli in liquid and agarose solidified media. Protoplasts that were isolated from young, morphogenic suspension cultures with moderate growth rates formed no or few microcalli. However, one suspension culture which retained the capcity for plant regeneration for more than 17 months yielded protoplasts which formed microcalli. From green shoots have been obtained in four experiments. Protoplast-derived plants have been established in soil." @default.
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- W2022436558 date "1989-01-01" @default.
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- W2022436558 title "Plant regeneration from suspension cultures and protoplasts of Lolium perenne L" @default.
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- W2022436558 doi "https://doi.org/10.1016/0168-9452(89)90242-2" @default.
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