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- W2022442015 abstract "A qualitative abnormality of the microheterogeneity of serum transferrin, demonstrated by isoelectric focusing, has previously been shown to be a highly specific indication of chronic alcoholism. The abnormality consists of a selective increase of a cathodal transferrin component which is probably caused by a reduction of the sialic acid content. The present study describes a method for quantitative estimation of the abnormal transferrin. The technique was based on analytical isoelectric focusing as the first step followed by direct immunofixation. The immunofixed transferrin was quantified by computerized on-line densitometry, and the transferrin abnormality was calculated as a quotient, where the amount of the cathodal component was expressed as a percentage of the relative total immunofixed transferrin quantity. This method was shown to possess high sensitivity and good reproducibility. In the controls the mean value of the quotient was 3.7%, while in the alcoholics it was 9.5% which was a highly significant difference (p<0.001). The possible functional significance of a disturbed sialic acid metabolism in alcoholism is discussed." @default.
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- W2022442015 title "Quantitative estimation of abnormal microheterogeneity of serum transferrin in alcoholics" @default.
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- W2022442015 doi "https://doi.org/10.1016/s0091-3057(80)80008-6" @default.
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