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- W2022451658 abstract "Derivatives of luteinizing hormone (LH) may retain activity in vitro, though not in vivo (Gospodarowicz, 1971; Leidenberger & Reichert, 1972). Assay methods for derivatives of LH are required for relating the structure of the LH molecule to its function. Two radioactive-ligand receptor assays are available (Catt, Dufau & Tsuruhara, 1971; Lee & Ryan, 1971). The following method is derived from the observation of Channing (1966, 1969) that monolayer cultures of granulosa cells produce progesterone in response to exogenous gonadotrophins. The gonadotrophins used were: purified human LH containing 5440 i.u./mg (Stockell Hartree, 1966); the M.R.C. gonadotrophin standard 69/104 (assumed potency 24 i.u. of LH and 10 i.u. of follicle-stimulating hormone (FSH)/ampoule); human chorionic gonadotrophin (HCG; Pregnyl, Organon) containing 1500 i.u./ampoule and human pituitary FSH containing 5600 (95% limits 4000–7850) i.u. FSH/mg by bioassay and 119 i.u. LH/mg by radioimmunoassay (batch CPDS/6 prepared in this laboratory). New Zealand White rabbits, aged 8" @default.
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- W2022451658 date "1973-08-01" @default.
- W2022451658 modified "2023-09-25" @default.
- W2022451658 title "PROGESTIN PRODUCTION IN VITRO BY RABBIT GRANULOSA CELLS AS AN ASSAY FOR LUTEINIZING HORMONE" @default.
- W2022451658 doi "https://doi.org/10.1677/joe.0.0580345" @default.
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