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- W2022459080 abstract "Abstract Sea urchin oocytes and mature unfertilized eggs synthesize 4 S RNA, histone mRNAs, mitochondrial RNAs, and heterodisperse RNAs. Incorporation into cytoplasmic ribosomal RNAs was not detected. Oocytes, but not mature eggs, synthesize heterogeneous, high-molecular-weight RNA. In both oocytes and eggs, a fraction of the newly synthesized histone mRNA is found on polyribosomes. About 50% of the histone mRNA which is pulse-labeled in oocytes is engaged with ribosomes, while the mature egg recruits only about 10% of the newly synthesized histone mRNA onto polysomes. Translation of histone mRNA in eggs was confirmed directly by showing that unfertilized eggs synthesize histones in vivo . Quantitatively, mitochondrial transcription contributes most prominently to the pattern of total RNA synthesis, accounting for approximately 70–90% of the [ 3 H]uridine incorporation by oocytes and eggs. Labeled mitochondrial RNAs include at least 10 discrete poly(A) + RNA species, presumably mRNAs, and 2 poly(A) − ribosomal RNAs. It is extremely difficult to completely eliminate all traces of labeled mitochondrial RNA from 15,000 g “postmitochondrial” supernatants in oocytes, eggs, and very early embryos: neither repeated pelleting at 15,000 g nor attempts to selectively inhibit mitochondrial transcription using ethidium bromide and acridine orange (which also depress nuclear transcription) were successful. These labeled mitochondrial RNAs, which routinely contaminate cytoplasmic 15,000 g supernatants, could pose problems for workers interested in analyzing the synthesis and utilization of abundant poly(A) + and poly(A) − RNAs in oocytes, eggs, and early embryos." @default.
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- W2022459080 title "RNA transcription and translation in sea urchin oocytes and eggs" @default.
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- W2022459080 doi "https://doi.org/10.1016/0012-1606(81)90285-2" @default.
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