Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022482017> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2022482017 endingPage "734" @default.
- W2022482017 startingPage "729" @default.
- W2022482017 abstract "Novel reversed micellar solutions formulated with a mixture of AOT (dioctyl sulfosuccinate) and DOLPA (dioleyl phosphoric acid) show good potential for use in reversed micellar protein extraction operations. Chymotrypsin is easily extracted from an aqueous phase into organic isooctane containing 10 mM AOT and DOLPA in a 4:1 ratio. The extraction ability of the mixed reversed micelles of 10 mM was higher than that of 200 mM AOT alone. The results of extraction indicated that the AOT-DOLPA mixed reversed micelles are very useful for separating and enriching chymotrypsin. Back-extraction of chymotrypsin from the organic phase to a fresh aqueous phase is also accomplished by adding an alcohol to the organic phase. Although the back-transfer of chymotrypsin from the reversed micelles formed by AOT alone is very slow and difficult, in the AOT-DOLPA mixed reversed micelles, the back-extraction can be achieved completely by addition of 10% (v/v) isobutyl alcohol to the reversed micellar phase. The time to attain to the equilibrium of back-extraction was reduced from more than 24 to 2 h by adding the alcohol. On the basis of the activity data, the best composition of AOT and DOLPA was a 4:1 ratio and the total surfactant concentration was 10 mM. The activity of chymotrypsin recovered from the mixed reversed micelles was higher than that of the initial protein before the forward-transfer. This result means that the novel mixed reversed micellar solutions are useful not only in separation but also in purification of proteins." @default.
- W2022482017 created "2016-06-24" @default.
- W2022482017 creator A5006140781 @default.
- W2022482017 creator A5037912117 @default.
- W2022482017 creator A5046348268 @default.
- W2022482017 creator A5049835634 @default.
- W2022482017 creator A5062256707 @default.
- W2022482017 date "1998-10-02" @default.
- W2022482017 modified "2023-09-23" @default.
- W2022482017 title "Extraction and Activity of Chymotrypsin Using AOT-DOLPA Mixed Reversed Micellar Systems" @default.
- W2022482017 doi "https://doi.org/10.1021/bp9800790" @default.
- W2022482017 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9758662" @default.
- W2022482017 hasPublicationYear "1998" @default.
- W2022482017 type Work @default.
- W2022482017 sameAs 2022482017 @default.
- W2022482017 citedByCount "36" @default.
- W2022482017 countsByYear W20224820172012 @default.
- W2022482017 countsByYear W20224820172014 @default.
- W2022482017 countsByYear W20224820172015 @default.
- W2022482017 countsByYear W20224820172017 @default.
- W2022482017 countsByYear W20224820172019 @default.
- W2022482017 countsByYear W20224820172020 @default.
- W2022482017 countsByYear W20224820172021 @default.
- W2022482017 crossrefType "journal-article" @default.
- W2022482017 hasAuthorship W2022482017A5006140781 @default.
- W2022482017 hasAuthorship W2022482017A5037912117 @default.
- W2022482017 hasAuthorship W2022482017A5046348268 @default.
- W2022482017 hasAuthorship W2022482017A5049835634 @default.
- W2022482017 hasAuthorship W2022482017A5062256707 @default.
- W2022482017 hasConcept C11268172 @default.
- W2022482017 hasConcept C149556010 @default.
- W2022482017 hasConcept C178790620 @default.
- W2022482017 hasConcept C181199279 @default.
- W2022482017 hasConcept C184651966 @default.
- W2022482017 hasConcept C185592680 @default.
- W2022482017 hasConcept C2778401398 @default.
- W2022482017 hasConcept C2780340462 @default.
- W2022482017 hasConcept C2781066024 @default.
- W2022482017 hasConcept C43617362 @default.
- W2022482017 hasConcept C44280652 @default.
- W2022482017 hasConcept C4725764 @default.
- W2022482017 hasConcept C5342626 @default.
- W2022482017 hasConcept C55493867 @default.
- W2022482017 hasConcept C58226133 @default.
- W2022482017 hasConceptScore W2022482017C11268172 @default.
- W2022482017 hasConceptScore W2022482017C149556010 @default.
- W2022482017 hasConceptScore W2022482017C178790620 @default.
- W2022482017 hasConceptScore W2022482017C181199279 @default.
- W2022482017 hasConceptScore W2022482017C184651966 @default.
- W2022482017 hasConceptScore W2022482017C185592680 @default.
- W2022482017 hasConceptScore W2022482017C2778401398 @default.
- W2022482017 hasConceptScore W2022482017C2780340462 @default.
- W2022482017 hasConceptScore W2022482017C2781066024 @default.
- W2022482017 hasConceptScore W2022482017C43617362 @default.
- W2022482017 hasConceptScore W2022482017C44280652 @default.
- W2022482017 hasConceptScore W2022482017C4725764 @default.
- W2022482017 hasConceptScore W2022482017C5342626 @default.
- W2022482017 hasConceptScore W2022482017C55493867 @default.
- W2022482017 hasConceptScore W2022482017C58226133 @default.
- W2022482017 hasIssue "5" @default.
- W2022482017 hasLocation W20224820171 @default.
- W2022482017 hasLocation W20224820172 @default.
- W2022482017 hasOpenAccess W2022482017 @default.
- W2022482017 hasPrimaryLocation W20224820171 @default.
- W2022482017 hasRelatedWork W1984684024 @default.
- W2022482017 hasRelatedWork W1991868791 @default.
- W2022482017 hasRelatedWork W2022482017 @default.
- W2022482017 hasRelatedWork W2022706171 @default.
- W2022482017 hasRelatedWork W2028740310 @default.
- W2022482017 hasRelatedWork W2031360492 @default.
- W2022482017 hasRelatedWork W2083660050 @default.
- W2022482017 hasRelatedWork W2367775492 @default.
- W2022482017 hasRelatedWork W2370152469 @default.
- W2022482017 hasRelatedWork W2385599040 @default.
- W2022482017 hasVolume "14" @default.
- W2022482017 isParatext "false" @default.
- W2022482017 isRetracted "false" @default.
- W2022482017 magId "2022482017" @default.
- W2022482017 workType "article" @default.