FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022488559> ?p ?o ?g. }

• W2022488559 endingPage "558" @default.
• W2022488559 startingPage "552" @default.
• W2022488559 abstract "Human exposure to carcinogenic alkylating agents can lead to the formation of covalently bound adducts in DNA, some of which are excreted in urine as alkylated purines following DNA degradation and repair. Tandem mass spectrometric methods have been developed for the qualitative and quantitative determination of such alkylpurines in human urine. Short-chain alkyl- and hydroxyalkylguanines have been synthesized with the substituents at the N-7-, O6- and N2-positions of guanine. Examination of the product ion scans of their molecular ions (electron impact (EI) ionization) revealed that the ion at m/z 151, [guanine]+, was common to all of the alkylguanines studied, with the exception of the methylated analogues. Precursor ion scans of this ion on partially purified human urine extracts showed the presence of several ions (e.g. m/z 179, 195) which were consistent with molecular ions for alkylguanines. The presence of these and other constituents was confirmed by product ion spectra of molecular ions (EI and fast atom bombardment), and by high-performance liquid chromatographic separation prior to tandem mass spectrometry (MS/MS). Evidence was obtained for the presence of N-7-methyl-, N2-dimethyl-, N2-dimethyl-, N2-ethyl- and N-7-(2-hydroxyethyl)guanine. Quantitative methods were established for these five alkyl guanines using gas chromatography mass spectrometry (GC/MS) and GC/MS/MS. Deuterated internal standards were synthesized and added to the urine prior to extraction of alkylpurines by Sep-Pak cartridge chromatography. The products were converted into their tert-butyldimethylsilyl derivatives and analysed by selected ion monitoring (SIM) of [M – 57]+ or by multiple reaction monitoring (MRM) of the fragmentation M+˙ → [M – 57]+. The MRM method yielded values for N-7-methylguanine of 2.57 ± S.D. 1.32 mg day−1 (n = 6), N2-methylguanine of 0.31 ± 0.10 mg day−1 (n = 10) and N2-dimethylguanine of 0.21 ± 0.23 mg day−1 (n = 10). N2-Ethyl- and N-7-(2-hydroxyethyl)guanine could only be detected by SIM at levels of ∼0.5 and 2 μg day−1, respectively. The MRM analyses, although inherently less sensitive than the SIM analyses, exhibit greater selectivity and consequently fewer contaminant ions." @default.
• W2022488559 created "2016-06-24" @default.
• W2022488559 creator A5040828120 @default.
• W2022488559 creator A5063582082 @default.
• W2022488559 creator A5079162506 @default.
• W2022488559 creator A5081200220 @default.
• W2022488559 date "1993-05-01" @default.
• W2022488559 modified "2023-09-26" @default.
• W2022488559 title "Tandem mass spectrometric approaches for the analysis of alkylguanines in human urine" @default.
• W2022488559 cites W1970985776 @default.
• W2022488559 cites W1990899455 @default.
• W2022488559 cites W1991924134 @default.
• W2022488559 cites W2001272685 @default.
• W2022488559 cites W2005846365 @default.
• W2022488559 cites W2006860328 @default.
• W2022488559 cites W2007283641 @default.
• W2022488559 cites W2015331089 @default.
• W2022488559 cites W2015819133 @default.
• W2022488559 cites W2017593462 @default.
• W2022488559 cites W2030225168 @default.
• W2022488559 cites W2036673001 @default.
• W2022488559 cites W2043942810 @default.
• W2022488559 cites W2046070614 @default.
• W2022488559 cites W2049900538 @default.
• W2022488559 cites W2058343722 @default.
• W2022488559 cites W2072083287 @default.
• W2022488559 cites W2083887678 @default.
• W2022488559 cites W2087550423 @default.
• W2022488559 cites W2217502273 @default.
• W2022488559 cites W2263826451 @default.
• W2022488559 cites W2332897344 @default.
• W2022488559 cites W87107169 @default.
• W2022488559 doi "https://doi.org/10.1002/oms.1210280514" @default.
• W2022488559 hasPublicationYear "1993" @default.
• W2022488559 type Work @default.
• W2022488559 sameAs 2022488559 @default.
• W2022488559 citedByCount "23" @default.
• W2022488559 countsByYear W20224885592012 @default.
• W2022488559 countsByYear W20224885592022 @default.
• W2022488559 crossrefType "journal-article" @default.
• W2022488559 hasAuthorship W2022488559A5040828120 @default.
• W2022488559 hasAuthorship W2022488559A5063582082 @default.
• W2022488559 hasAuthorship W2022488559A5079162506 @default.
• W2022488559 hasAuthorship W2022488559A5081200220 @default.
• W2022488559 hasConcept C104317684 @default.
• W2022488559 hasConcept C108204754 @default.
• W2022488559 hasConcept C145148216 @default.
• W2022488559 hasConcept C162356407 @default.
• W2022488559 hasConcept C178790620 @default.
• W2022488559 hasConcept C185592680 @default.
• W2022488559 hasConcept C198291218 @default.
• W2022488559 hasConcept C199033913 @default.
• W2022488559 hasConcept C205759337 @default.
• W2022488559 hasConcept C2778301229 @default.
• W2022488559 hasConcept C2780026642 @default.
• W2022488559 hasConcept C2780263894 @default.
• W2022488559 hasConcept C31827203 @default.
• W2022488559 hasConcept C40325409 @default.
• W2022488559 hasConcept C43617362 @default.
• W2022488559 hasConcept C512185932 @default.
• W2022488559 hasConcept C51884965 @default.
• W2022488559 hasConcept C55493867 @default.
• W2022488559 hasConcept C66594872 @default.
• W2022488559 hasConceptScore W2022488559C104317684 @default.
• W2022488559 hasConceptScore W2022488559C108204754 @default.
• W2022488559 hasConceptScore W2022488559C145148216 @default.
• W2022488559 hasConceptScore W2022488559C162356407 @default.
• W2022488559 hasConceptScore W2022488559C178790620 @default.
• W2022488559 hasConceptScore W2022488559C185592680 @default.
• W2022488559 hasConceptScore W2022488559C198291218 @default.
• W2022488559 hasConceptScore W2022488559C199033913 @default.
• W2022488559 hasConceptScore W2022488559C205759337 @default.
• W2022488559 hasConceptScore W2022488559C2778301229 @default.
• W2022488559 hasConceptScore W2022488559C2780026642 @default.
• W2022488559 hasConceptScore W2022488559C2780263894 @default.
• W2022488559 hasConceptScore W2022488559C31827203 @default.
• W2022488559 hasConceptScore W2022488559C40325409 @default.
• W2022488559 hasConceptScore W2022488559C43617362 @default.
• W2022488559 hasConceptScore W2022488559C512185932 @default.
• W2022488559 hasConceptScore W2022488559C51884965 @default.
• W2022488559 hasConceptScore W2022488559C55493867 @default.
• W2022488559 hasConceptScore W2022488559C66594872 @default.
• W2022488559 hasIssue "5" @default.
• W2022488559 hasLocation W20224885591 @default.
• W2022488559 hasOpenAccess W2022488559 @default.
• W2022488559 hasPrimaryLocation W20224885591 @default.
• W2022488559 hasRelatedWork W2031782598 @default.
• W2022488559 hasRelatedWork W2033316405 @default.
• W2022488559 hasRelatedWork W2040842071 @default.
• W2022488559 hasRelatedWork W2050604471 @default.
• W2022488559 hasRelatedWork W2054603492 @default.
• W2022488559 hasRelatedWork W2063966071 @default.
• W2022488559 hasRelatedWork W2076060861 @default.
• W2022488559 hasRelatedWork W2094162387 @default.
• W2022488559 hasRelatedWork W2350270485 @default.
• W2022488559 hasRelatedWork W3011948259 @default.
• W2022488559 hasVolume "28" @default.
• W2022488559 isParatext "false" @default.

• next