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- W2022514573 abstract "Salmonella enterica subsp. enterica serovar Virchow has been recognized as a significant health burden in Asia, Australia and Europe. In addition to its global distribution, S. Virchow is clinically significant due to the frequency at which it causes invasive infections and its association with outbreaks arising from food-borne transmission. Here, we examine the genome of an invasive isolate of S. Virchow SVQ1 (phage type 8) from an outbreak in southeast Queensland, Australia. In addition to identifying new potential genotyping targets that could be used for discriminating between S. Virchow strains in outbreak scenarios, we also aimed to carry out a comprehensive comparative analysis of the S. Virchow genomes. Genome comparisons between S. Virchow SVQ1 and S. Virchow SL491, a previously published strain, identified a high degree of genomic similarity between the two strains with fewer than 200 single nucleotide differences. Clustered Regularly Interspaced Palindromic Repeats (CRISPR) regions were identified as a highly variable region that could be used to discriminate between S. Virchow isolates. We amplified and sequenced the CRISPR regions of fifteen S. Virchow isolates collected from seven different outbreaks across Australia. We observed three allelic types of the CRISPR region from these isolates based on the presence/absence of the spacers and were able to discriminate S. Virchow phage type 8 isolates originating from different outbreaks. A comparison with 27 published Salmonella genomes found that the S. Virchow SVQ1 genome encodes 11 previously described Salmonella Pathogenicity Islands (SPI), as well as additional genomic islands including a remnant integrative conjugative element that is distinct from SPI-7. In addition, the S. Virchow genome possesses a novel prophage that encodes the Type III secretion system effector protein SopE, a key Salmonella virulence factor. The prophage shares very little similarity to the SopE prophages found in other Salmonella serovars suggesting an independent acquisition of sopE. The availability of this genome will serve as a genome template and facilitate further studies on understanding the virulence and global distribution of the S. Virchow serovar, as well as the development of genotyping methods for outbreak investigations." @default.
- W2022514573 created "2016-06-24" @default.
- W2022514573 creator A5015776019 @default.
- W2022514573 creator A5018759867 @default.
- W2022514573 creator A5022370405 @default.
- W2022514573 creator A5043626601 @default.
- W2022514573 creator A5055418631 @default.
- W2022514573 creator A5087445309 @default.
- W2022514573 date "2014-05-21" @default.
- W2022514573 modified "2023-10-09" @default.
- W2022514573 title "Genome analysis and CRISPR typing of Salmonella enterica serovar Virchow" @default.
- W2022514573 cites W1559862260 @default.
- W2022514573 cites W1597150871 @default.
- W2022514573 cites W180454672 @default.
- W2022514573 cites W1965316555 @default.
- W2022514573 cites W1965404910 @default.
- W2022514573 cites W1966534922 @default.
- W2022514573 cites W1967412126 @default.
- W2022514573 cites W1980189827 @default.
- W2022514573 cites W1981039975 @default.
- W2022514573 cites W1982369197 @default.
- W2022514573 cites W1984710779 @default.
- W2022514573 cites W1989983512 @default.
- W2022514573 cites W1997207082 @default.
- W2022514573 cites W1997933100 @default.
- W2022514573 cites W2008376419 @default.
- W2022514573 cites W2010986636 @default.
- W2022514573 cites W2015213608 @default.
- W2022514573 cites W2021671330 @default.
- W2022514573 cites W2021800618 @default.
- W2022514573 cites W2022873862 @default.
- W2022514573 cites W2026425987 @default.
- W2022514573 cites W2026741583 @default.
- W2022514573 cites W2029893373 @default.
- W2022514573 cites W2031719990 @default.
- W2022514573 cites W2033380982 @default.
- W2022514573 cites W2034542302 @default.
- W2022514573 cites W2038417044 @default.
- W2022514573 cites W2038662968 @default.
- W2022514573 cites W2041148890 @default.
- W2022514573 cites W2043623479 @default.
- W2022514573 cites W2046137036 @default.
- W2022514573 cites W2046713838 @default.
- W2022514573 cites W2050169817 @default.
- W2022514573 cites W2050688784 @default.
- W2022514573 cites W2055043387 @default.
- W2022514573 cites W2060573994 @default.
- W2022514573 cites W2066910097 @default.
- W2022514573 cites W2069678357 @default.
- W2022514573 cites W2077943995 @default.
- W2022514573 cites W2081209254 @default.
- W2022514573 cites W2083145206 @default.
- W2022514573 cites W2083537575 @default.
- W2022514573 cites W2083739951 @default.
- W2022514573 cites W2091030812 @default.
- W2022514573 cites W2091270609 @default.
- W2022514573 cites W2095685450 @default.
- W2022514573 cites W2095951850 @default.
- W2022514573 cites W2096069224 @default.
- W2022514573 cites W2097155272 @default.
- W2022514573 cites W2097802511 @default.
- W2022514573 cites W2098417493 @default.
- W2022514573 cites W2100363512 @default.
- W2022514573 cites W2102601964 @default.
- W2022514573 cites W2103461849 @default.
- W2022514573 cites W2104178347 @default.
- W2022514573 cites W2105537192 @default.
- W2022514573 cites W2114853012 @default.
- W2022514573 cites W2116106444 @default.
- W2022514573 cites W2116180472 @default.
- W2022514573 cites W2116548822 @default.
- W2022514573 cites W2116924408 @default.
- W2022514573 cites W2119730621 @default.
- W2022514573 cites W2120463627 @default.
- W2022514573 cites W2121467305 @default.
- W2022514573 cites W2124574960 @default.
- W2022514573 cites W2125650558 @default.
- W2022514573 cites W2128352784 @default.
- W2022514573 cites W2128899058 @default.
- W2022514573 cites W2132295158 @default.
- W2022514573 cites W2132530250 @default.
- W2022514573 cites W2133300547 @default.
- W2022514573 cites W2133790733 @default.
- W2022514573 cites W2135133625 @default.
- W2022514573 cites W2135146244 @default.
- W2022514573 cites W2139201774 @default.
- W2022514573 cites W2141932030 @default.
- W2022514573 cites W2143936572 @default.
- W2022514573 cites W2151865733 @default.
- W2022514573 cites W2156273926 @default.
- W2022514573 cites W2157295579 @default.
- W2022514573 cites W2164075646 @default.
- W2022514573 cites W2165645605 @default.
- W2022514573 cites W2169962614 @default.
- W2022514573 cites W2170488656 @default.
- W2022514573 cites W2172261107 @default.
- W2022514573 cites W2194204466 @default.
- W2022514573 cites W4210623056 @default.
- W2022514573 cites W4250052351 @default.
- W2022514573 doi "https://doi.org/10.1186/1471-2164-15-389" @default.