Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022523666> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2022523666 abstract "Recent data suggest an important role for calcium (Ca2+) in human placental endocrinology. Thus, the regulation of Ca2+ influx seems to be implicated in the modulation of human placental lactogen and hCG release. A possible mechanism of influx regulation is through receptor-operated channels. One of the most characterized receptor gating Ca2+ channels, the ATP receptor, stimulates the intracellular calcium concentration ([Ca2+]i) in various tissues. The aim of this study was to determine whether ATP receptors gating Ca2+ channels are also present in placental cells. We thus determined the effect of ATP on [Ca2+]i in human term trophoblastic cells loaded with the Ca(2+)-responsive fluorescent dye fura-2. ATP stimulated a 4.3 +/- 0.4 (+/- SE)-fold increase in [Ca2+]i, with a half-maximal effective concentration (EC50) of 1.5 mumol/L. The pharmacological activation profile suggests the presence of purinergic P2u receptors (nucleotide receptors), because uridine 5'-triphosphate (UTP) also stimulated [Ca2+]i (4.0-fold increase, with an EC50 of 10 mumol/L). The ATP-stimulated [Ca2+]i was partly sensitive to pertussis toxin; we observed a 58% inhibition of ATP-induced [Ca2+]i with the toxin without effect on basal [Ca2+]i. The ATP- and UTP-stimulated [Ca2+]i declined with time in the presence of ATP (or UTP). The rate of deactivation was rapid (t1/2, < 60 s with 10(-5) mol/L ATP) and concentration dependent. The deactivation occurring during one application of ATP or UTP resulted in a diminution of subsequent responses. The recovery was incomplete even with long waiting times (up to 30 min). ATP and UTP also stimulated inositol phosphate production with EC50 values of 11 and 15 mumol/L, respectively, but not human placental lactogen or hCG release in experiments in which known secretagogues were effective. The results suggest the presence in human term placental cells of P2u receptors pharmacologically similar to those observed in other tissues, especially in the pituitary and amnion. The physiological significance of this stimulation of [Ca2+]i by ATP and UTP in the human placenta remains to be investigated." @default.
- W2022523666 created "2016-06-24" @default.
- W2022523666 creator A5012572436 @default.
- W2022523666 creator A5025932075 @default.
- W2022523666 date "1995-06-01" @default.
- W2022523666 modified "2023-09-30" @default.
- W2022523666 title "Stimulation of intracellular calcium concentration by adenosine triphosphate and uridine 5'-triphosphate in human term placental cells: evidence for purinergic receptors." @default.
- W2022523666 doi "https://doi.org/10.1210/jcem.80.6.7775628" @default.
- W2022523666 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7775628" @default.
- W2022523666 hasPublicationYear "1995" @default.
- W2022523666 type Work @default.
- W2022523666 sameAs 2022523666 @default.
- W2022523666 citedByCount "9" @default.
- W2022523666 countsByYear W20225236662012 @default.
- W2022523666 crossrefType "journal-article" @default.
- W2022523666 hasAuthorship W2022523666A5012572436 @default.
- W2022523666 hasAuthorship W2022523666A5025932075 @default.
- W2022523666 hasConcept C104317684 @default.
- W2022523666 hasConcept C126322002 @default.
- W2022523666 hasConcept C134018914 @default.
- W2022523666 hasConcept C160225129 @default.
- W2022523666 hasConcept C170493617 @default.
- W2022523666 hasConcept C181080969 @default.
- W2022523666 hasConcept C181199279 @default.
- W2022523666 hasConcept C185592680 @default.
- W2022523666 hasConcept C24998067 @default.
- W2022523666 hasConcept C2776991684 @default.
- W2022523666 hasConcept C2778470358 @default.
- W2022523666 hasConcept C2778564976 @default.
- W2022523666 hasConcept C2779564974 @default.
- W2022523666 hasConcept C49773422 @default.
- W2022523666 hasConcept C512185932 @default.
- W2022523666 hasConcept C519063684 @default.
- W2022523666 hasConcept C55493867 @default.
- W2022523666 hasConcept C57306754 @default.
- W2022523666 hasConcept C71924100 @default.
- W2022523666 hasConcept C79879829 @default.
- W2022523666 hasConcept C80631254 @default.
- W2022523666 hasConcept C86803240 @default.
- W2022523666 hasConcept C98539663 @default.
- W2022523666 hasConceptScore W2022523666C104317684 @default.
- W2022523666 hasConceptScore W2022523666C126322002 @default.
- W2022523666 hasConceptScore W2022523666C134018914 @default.
- W2022523666 hasConceptScore W2022523666C160225129 @default.
- W2022523666 hasConceptScore W2022523666C170493617 @default.
- W2022523666 hasConceptScore W2022523666C181080969 @default.
- W2022523666 hasConceptScore W2022523666C181199279 @default.
- W2022523666 hasConceptScore W2022523666C185592680 @default.
- W2022523666 hasConceptScore W2022523666C24998067 @default.
- W2022523666 hasConceptScore W2022523666C2776991684 @default.
- W2022523666 hasConceptScore W2022523666C2778470358 @default.
- W2022523666 hasConceptScore W2022523666C2778564976 @default.
- W2022523666 hasConceptScore W2022523666C2779564974 @default.
- W2022523666 hasConceptScore W2022523666C49773422 @default.
- W2022523666 hasConceptScore W2022523666C512185932 @default.
- W2022523666 hasConceptScore W2022523666C519063684 @default.
- W2022523666 hasConceptScore W2022523666C55493867 @default.
- W2022523666 hasConceptScore W2022523666C57306754 @default.
- W2022523666 hasConceptScore W2022523666C71924100 @default.
- W2022523666 hasConceptScore W2022523666C79879829 @default.
- W2022523666 hasConceptScore W2022523666C80631254 @default.
- W2022523666 hasConceptScore W2022523666C86803240 @default.
- W2022523666 hasConceptScore W2022523666C98539663 @default.
- W2022523666 hasLocation W20225236661 @default.
- W2022523666 hasLocation W20225236662 @default.
- W2022523666 hasOpenAccess W2022523666 @default.
- W2022523666 hasPrimaryLocation W20225236661 @default.
- W2022523666 hasRelatedWork W182842989 @default.
- W2022523666 hasRelatedWork W1985788361 @default.
- W2022523666 hasRelatedWork W2000735821 @default.
- W2022523666 hasRelatedWork W2030096204 @default.
- W2022523666 hasRelatedWork W2048847665 @default.
- W2022523666 hasRelatedWork W2063879185 @default.
- W2022523666 hasRelatedWork W2073098137 @default.
- W2022523666 hasRelatedWork W2150030148 @default.
- W2022523666 hasRelatedWork W2315348372 @default.
- W2022523666 hasRelatedWork W2331369014 @default.
- W2022523666 isParatext "false" @default.
- W2022523666 isRetracted "false" @default.
- W2022523666 magId "2022523666" @default.
- W2022523666 workType "article" @default.