Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022582854> ?p ?o ?g. }
- W2022582854 endingPage "e20789" @default.
- W2022582854 startingPage "e20789" @default.
- W2022582854 abstract "Highly selective, cell-permeable and fast-acting inhibitors of individual kinases are sought-after as tools for studying the cellular function of kinases in real time. A combination of small molecule synthesis and protein mutagenesis, identified a highly potent inhibitor (1-Isopropyl-3-(phenylethynyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine) of a rationally engineered Hog1 serine/threonine kinase (Hog1(T100G)). This inhibitor has been successfully used to study various aspects of Hog1 signaling, including a transient cell cycle arrest and gene expression changes mediated by Hog1 in response to stress. This study also underscores that the general applicability of this approach depends, in part, on the selectivity of the designed the inhibitor with respect to activity versus the engineered and wild type kinases. To explore this specificity in detail, we used a validated chemogenetic assay to assess the effect of this inhibitor on all gene products in yeast in parallel. The results from this screen emphasize the need for caution and for case-by-case assessment when using the Analog-Sensitive Kinase Allele technology to assess the physiological roles of kinases." @default.
- W2022582854 created "2016-06-24" @default.
- W2022582854 creator A5027866777 @default.
- W2022582854 creator A5036078839 @default.
- W2022582854 creator A5045012079 @default.
- W2022582854 creator A5049311354 @default.
- W2022582854 creator A5053608829 @default.
- W2022582854 creator A5069396293 @default.
- W2022582854 creator A5072254414 @default.
- W2022582854 creator A5084599373 @default.
- W2022582854 creator A5091395541 @default.
- W2022582854 creator A5091821428 @default.
- W2022582854 date "2011-06-17" @default.
- W2022582854 modified "2023-10-13" @default.
- W2022582854 title "Design, Synthesis and Characterization of a Highly Effective Inhibitor for Analog-Sensitive (as) Kinases" @default.
- W2022582854 cites W1520738779 @default.
- W2022582854 cites W1964364756 @default.
- W2022582854 cites W1974619322 @default.
- W2022582854 cites W1988506250 @default.
- W2022582854 cites W2002303369 @default.
- W2022582854 cites W2008191493 @default.
- W2022582854 cites W2014545373 @default.
- W2022582854 cites W2024090733 @default.
- W2022582854 cites W2024264816 @default.
- W2022582854 cites W2026792394 @default.
- W2022582854 cites W2044933943 @default.
- W2022582854 cites W2046822015 @default.
- W2022582854 cites W2069405322 @default.
- W2022582854 cites W2071907071 @default.
- W2022582854 cites W2080465722 @default.
- W2022582854 cites W2088270869 @default.
- W2022582854 cites W2090895449 @default.
- W2022582854 cites W2097032828 @default.
- W2022582854 cites W2097948046 @default.
- W2022582854 cites W2105229705 @default.
- W2022582854 cites W2108357729 @default.
- W2022582854 cites W2118390137 @default.
- W2022582854 cites W2128761585 @default.
- W2022582854 cites W2134582937 @default.
- W2022582854 cites W2138072853 @default.
- W2022582854 cites W2141879066 @default.
- W2022582854 cites W2156460914 @default.
- W2022582854 cites W2159100436 @default.
- W2022582854 cites W2159675211 @default.
- W2022582854 cites W2171109336 @default.
- W2022582854 cites W2179928471 @default.
- W2022582854 cites W2945198366 @default.
- W2022582854 cites W2951789515 @default.
- W2022582854 cites W2952139546 @default.
- W2022582854 cites W68895299 @default.
- W2022582854 doi "https://doi.org/10.1371/journal.pone.0020789" @default.
- W2022582854 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3117834" @default.
- W2022582854 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21698101" @default.
- W2022582854 hasPublicationYear "2011" @default.
- W2022582854 type Work @default.
- W2022582854 sameAs 2022582854 @default.
- W2022582854 citedByCount "9" @default.
- W2022582854 countsByYear W20225828542012 @default.
- W2022582854 countsByYear W20225828542013 @default.
- W2022582854 countsByYear W20225828542014 @default.
- W2022582854 countsByYear W20225828542015 @default.
- W2022582854 countsByYear W20225828542017 @default.
- W2022582854 countsByYear W20225828542020 @default.
- W2022582854 crossrefType "journal-article" @default.
- W2022582854 hasAuthorship W2022582854A5027866777 @default.
- W2022582854 hasAuthorship W2022582854A5036078839 @default.
- W2022582854 hasAuthorship W2022582854A5045012079 @default.
- W2022582854 hasAuthorship W2022582854A5049311354 @default.
- W2022582854 hasAuthorship W2022582854A5053608829 @default.
- W2022582854 hasAuthorship W2022582854A5069396293 @default.
- W2022582854 hasAuthorship W2022582854A5072254414 @default.
- W2022582854 hasAuthorship W2022582854A5084599373 @default.
- W2022582854 hasAuthorship W2022582854A5091395541 @default.
- W2022582854 hasAuthorship W2022582854A5091821428 @default.
- W2022582854 hasBestOaLocation W20225828541 @default.
- W2022582854 hasConcept C104317684 @default.
- W2022582854 hasConcept C11960822 @default.
- W2022582854 hasConcept C14036430 @default.
- W2022582854 hasConcept C143065580 @default.
- W2022582854 hasConcept C16318435 @default.
- W2022582854 hasConcept C184235292 @default.
- W2022582854 hasConcept C185592680 @default.
- W2022582854 hasConcept C2776414213 @default.
- W2022582854 hasConcept C2910050888 @default.
- W2022582854 hasConcept C55493867 @default.
- W2022582854 hasConcept C86803240 @default.
- W2022582854 hasConcept C95444343 @default.
- W2022582854 hasConcept C97029542 @default.
- W2022582854 hasConceptScore W2022582854C104317684 @default.
- W2022582854 hasConceptScore W2022582854C11960822 @default.
- W2022582854 hasConceptScore W2022582854C14036430 @default.
- W2022582854 hasConceptScore W2022582854C143065580 @default.
- W2022582854 hasConceptScore W2022582854C16318435 @default.
- W2022582854 hasConceptScore W2022582854C184235292 @default.
- W2022582854 hasConceptScore W2022582854C185592680 @default.
- W2022582854 hasConceptScore W2022582854C2776414213 @default.
- W2022582854 hasConceptScore W2022582854C2910050888 @default.
- W2022582854 hasConceptScore W2022582854C55493867 @default.