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- W2022594474 abstract "A homology-based cloning strategy yielded Sdga, a cDNA clone presumably encoding α-subunit of heterotrimeric guanosine 5′-triphosphate-binding protein complex, from leaf tissues of Scoparia dulcis. Phylogenetic tree analysis of G-protein α-subunits from various biological sources suggested that, unlike in animal cells, classification of Gα-proteins into specific subfamilies could not be applicable to the proteins from higher plants. Restriction digests of genomic DNA of S. dulcis showed a single hybridized signal in Southern blot analysis, suggesting that Sdga is a sole gene encoding Gα-subunit in this plant. The expression level of Sdga appeared to be maintained at almost constant level after exposure of the leaves to methyl jasmonate as analyzed by reverse-transcription polymerase chain reaction. These results suggest that Sdga plays roles in methyl jasmonate-induced responses of S. dulcis without a notable change in the transcriptional level." @default.
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- W2022594474 date "2008-01-01" @default.
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- W2022594474 title "Cloning and Characterization of Sdga Gene Encoding .ALPHA.-Subunit of Heterotrimeric Guanosine 5'-Triphosphate-Binding Protein Complex in Scoparia dulcis" @default.
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- W2022594474 doi "https://doi.org/10.1248/bpb.31.2150" @default.
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