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- W2022602362 abstract "The apoenzyme and holoenzyme (NADP+ complex) of human placental 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) were prepared from affinity chromatography using various elutions by column liquid chromatography. The apoenzyme was obtained using NAD+ elution in a Blue-Sepharose column, followed by NAD+ separation on a Phenyl-Superose hydrophobic-interaction or a Mono Q anion-exchange column. The 17 beta-HSD-NADP+ complex was prepared using NADP+ elution in a Blue-Sepharose column. The two forms have different A280/A260 ratios and are suitable for further study of enzyme-cofactor interactions." @default.
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- W2022602362 date "1993-04-01" @default.
- W2022602362 modified "2023-10-16" @default.
- W2022602362 title "Preparation of the apoenzyme and holoenzyme forms of human 17β-hydroxysteroid dehydrogenase" @default.
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- W2022602362 doi "https://doi.org/10.1016/0378-4347(93)80236-w" @default.
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