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• W2022603290 abstract "Phosphorescent platinum(II) coproporphyrin label (PtCP) is evaluated for the detection of cellular proteases by time-resolved fluorescence in homogeneous format. An octameric peptide containing the recognition motif for the caspase-3 enzyme was dual labeled with a new maleimide derivative of PtCP and with the dark quencher dabcyl. Following photophysical characterization, the quenched substrate was employed in cleavage assays for caspase-3 using Jurkat and HL60 cell lines treated with proapoptotic stimuli performed on a commercial plate reader. Dose–response and time course assays for the drug camptothecin were obtained for comparison with conventional fluorometric detection." @default.
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• W2022603290 date "2005-07-01" @default.
• W2022603290 modified "2023-09-24" @default.
• W2022603290 title "Homogeneous assays for cellular proteases employing the platinum(II)–coproporphyrin label and time-resolved phosphorescence" @default.
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• W2022603290 doi "https://doi.org/10.1016/j.ab.2005.04.016" @default.
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