Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022604327> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2022604327 endingPage "87" @default.
- W2022604327 startingPage "80" @default.
- W2022604327 abstract "The aim of this study was to develop plasma-polymerized surfaces suitable for the attachment and culture of human keratinocytes and that would allow their subsequent transfer to a wound-bed model. Keratinocyte attachment has been assessed on a carrier polymer, either untreated or treated with a hydrocarbon plasma polymer, collagen I, or carboxylic-acid-containing plasma copolymers. Cell attachment was poor on the bare carrier polymer and hydrocarbon plasma polymer (PP) surfaces. Cell attachment was good and comparable on collagen I-coated carrier polymer and carrier polymer plasma coated with carboxylic acid functionalities. After 24 h of cell culture, surfaces were inverted so that cells were adjacent to a de-epidermalized dermis (DED) for 4 days. After 4 days in contact with DED, the surfaces were removed and the level of residual cells and cells transferred to DED were assessed using a cell viability assay. Cell transfer from the collagen I-coated surface was on the order of 90%. Transfer from the carrier polymer surface and the hydrocarbon-coated surface was poor while cells cultured on acid-containing surfaces showed high levels of transfer. Cell transfer was greatest from those surfaces containing the highest level of acid functionality (ca. 21%). Cell transfer was not significantly affected by the choice of carrier polymer material although some sample-to-sample variation was seen. To determine that plasma-polymerized surfaces could be used clinically, selected samples were sterilized with ethylene oxide. Subsequent analysis and cell culture indicated that the surface chemistry and cell-transfer capability of these plasma-polymerized surfaces were unaffected by the sterilization procedure. Plasma-polymerized carboxylic-acid-containing surfaces show great promise in the field of wound healing, encouraging keratinocyte attachment and permitting keratinocyte transfer to a wound bed." @default.
- W2022604327 created "2016-06-24" @default.
- W2022604327 creator A5003159963 @default.
- W2022604327 creator A5049275138 @default.
- W2022604327 creator A5058736479 @default.
- W2022604327 creator A5066381082 @default.
- W2022604327 creator A5072742692 @default.
- W2022604327 date "2002-11-01" @default.
- W2022604327 modified "2023-10-17" @default.
- W2022604327 title "Plasma-polymerized surfaces for culture of human keratinocytes and transfer of cells to anin vitro wound-bed model" @default.
- W2022604327 cites W1977433184 @default.
- W2022604327 cites W1985528214 @default.
- W2022604327 cites W1997016871 @default.
- W2022604327 cites W2000964047 @default.
- W2022604327 cites W2014901216 @default.
- W2022604327 cites W2020464515 @default.
- W2022604327 cites W2030951059 @default.
- W2022604327 cites W2031129554 @default.
- W2022604327 cites W2061838858 @default.
- W2022604327 cites W2077081316 @default.
- W2022604327 cites W2090106568 @default.
- W2022604327 cites W2127827743 @default.
- W2022604327 cites W2138585783 @default.
- W2022604327 cites W2158354288 @default.
- W2022604327 cites W2159528256 @default.
- W2022604327 cites W4231604197 @default.
- W2022604327 doi "https://doi.org/10.1002/jbm.a.10356" @default.
- W2022604327 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12483699" @default.
- W2022604327 hasPublicationYear "2002" @default.
- W2022604327 type Work @default.
- W2022604327 sameAs 2022604327 @default.
- W2022604327 citedByCount "87" @default.
- W2022604327 countsByYear W20226043272012 @default.
- W2022604327 countsByYear W20226043272013 @default.
- W2022604327 countsByYear W20226043272014 @default.
- W2022604327 countsByYear W20226043272015 @default.
- W2022604327 countsByYear W20226043272016 @default.
- W2022604327 countsByYear W20226043272017 @default.
- W2022604327 countsByYear W20226043272018 @default.
- W2022604327 countsByYear W20226043272020 @default.
- W2022604327 countsByYear W20226043272021 @default.
- W2022604327 countsByYear W20226043272022 @default.
- W2022604327 countsByYear W20226043272023 @default.
- W2022604327 crossrefType "journal-article" @default.
- W2022604327 hasAuthorship W2022604327A5003159963 @default.
- W2022604327 hasAuthorship W2022604327A5049275138 @default.
- W2022604327 hasAuthorship W2022604327A5058736479 @default.
- W2022604327 hasAuthorship W2022604327A5066381082 @default.
- W2022604327 hasAuthorship W2022604327A5072742692 @default.
- W2022604327 hasConcept C115545448 @default.
- W2022604327 hasConcept C12554922 @default.
- W2022604327 hasConcept C127413603 @default.
- W2022604327 hasConcept C159985019 @default.
- W2022604327 hasConcept C185592680 @default.
- W2022604327 hasConcept C188027245 @default.
- W2022604327 hasConcept C192562407 @default.
- W2022604327 hasConcept C42360764 @default.
- W2022604327 hasConcept C44228677 @default.
- W2022604327 hasConcept C521977710 @default.
- W2022604327 hasConcept C86803240 @default.
- W2022604327 hasConceptScore W2022604327C115545448 @default.
- W2022604327 hasConceptScore W2022604327C12554922 @default.
- W2022604327 hasConceptScore W2022604327C127413603 @default.
- W2022604327 hasConceptScore W2022604327C159985019 @default.
- W2022604327 hasConceptScore W2022604327C185592680 @default.
- W2022604327 hasConceptScore W2022604327C188027245 @default.
- W2022604327 hasConceptScore W2022604327C192562407 @default.
- W2022604327 hasConceptScore W2022604327C42360764 @default.
- W2022604327 hasConceptScore W2022604327C44228677 @default.
- W2022604327 hasConceptScore W2022604327C521977710 @default.
- W2022604327 hasConceptScore W2022604327C86803240 @default.
- W2022604327 hasIssue "1" @default.
- W2022604327 hasLocation W20226043271 @default.
- W2022604327 hasLocation W20226043272 @default.
- W2022604327 hasOpenAccess W2022604327 @default.
- W2022604327 hasPrimaryLocation W20226043271 @default.
- W2022604327 hasRelatedWork W1952664864 @default.
- W2022604327 hasRelatedWork W1972967867 @default.
- W2022604327 hasRelatedWork W2028023233 @default.
- W2022604327 hasRelatedWork W2349236722 @default.
- W2022604327 hasRelatedWork W2359267066 @default.
- W2022604327 hasRelatedWork W2484680398 @default.
- W2022604327 hasRelatedWork W3103565007 @default.
- W2022604327 hasRelatedWork W3173755470 @default.
- W2022604327 hasRelatedWork W579750849 @default.
- W2022604327 hasRelatedWork W623819857 @default.
- W2022604327 hasVolume "64A" @default.
- W2022604327 isParatext "false" @default.
- W2022604327 isRetracted "false" @default.
- W2022604327 magId "2022604327" @default.
- W2022604327 workType "article" @default.