Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022630006> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2022630006 endingPage "341" @default.
- W2022630006 startingPage "321" @default.
- W2022630006 abstract "We are developing a genetic map of the dog based partly upon markers contained within known genes. In order to facilitate the development of these markers, we have used polymerase chain reaction (PCR) primers designed to conserved regions of genes that have been sequenced in at least two species. We have refined the method for designing primers to maximize the number that produce successful amplifications across as many mammalian species as possible. We report the development of primer sets for 11 loci in detail:CFTR, COL10A1, CSFIR, CYP1A1, DCN1, FES, GHR, GLB1, PKLR, PVALB, andRB1. We also report an additional 75 primer sets in the appendices. The PCR products were sequenced to show that the primers amplify the expected canine genes. These primer sets thus define a class of gene-specific sequence-tagged sites (STSs). There are a number of uses for these STSs, including the rapid development of various linkage tools and the rapid testing of genomic and cDNA libraries for the presence of their corresponding genes. Six of the eleven gene targets reported in detail have been proposed to serve as “anchored reference loci” for the development of mammalian genetic maps [O'Brien, S. J.,et al., Nat. Genet. 3:103, 1993]. The primer sets should cover a significant portion of the canine genome for the development of a linkage map. In order to determine how useful these primer sets would be for the other genome projects, we tested the 11 primer sets on the DNA from species representing five mammalian orders. Eighty-four percent of the gene-species combinations amplified successfully. We have named these primer sets “universal mammalian sequence-tagged sites” because they should be useful for many mammalian genome projects." @default.
- W2022630006 created "2016-06-24" @default.
- W2022630006 creator A5045488672 @default.
- W2022630006 creator A5062501052 @default.
- W2022630006 creator A5066564174 @default.
- W2022630006 creator A5085854115 @default.
- W2022630006 date "1996-08-01" @default.
- W2022630006 modified "2023-10-17" @default.
- W2022630006 title "Gene-specific universal mammalian sequence-tagged sites: Application to the canine genome" @default.
- W2022630006 cites W1978910518 @default.
- W2022630006 cites W1987465261 @default.
- W2022630006 cites W1998952262 @default.
- W2022630006 cites W1999642472 @default.
- W2022630006 cites W2005988643 @default.
- W2022630006 cites W2006698454 @default.
- W2022630006 cites W2009095852 @default.
- W2022630006 cites W2012307531 @default.
- W2022630006 cites W2027588766 @default.
- W2022630006 cites W2031914648 @default.
- W2022630006 cites W2040219510 @default.
- W2022630006 cites W2042680084 @default.
- W2022630006 cites W2056537262 @default.
- W2022630006 cites W2061941091 @default.
- W2022630006 cites W2064095396 @default.
- W2022630006 cites W2074778021 @default.
- W2022630006 cites W2080385955 @default.
- W2022630006 cites W2098533677 @default.
- W2022630006 cites W2117698343 @default.
- W2022630006 cites W2121440003 @default.
- W2022630006 cites W2134720561 @default.
- W2022630006 cites W2147275864 @default.
- W2022630006 cites W2148353957 @default.
- W2022630006 cites W2252361719 @default.
- W2022630006 cites W2298917175 @default.
- W2022630006 cites W2399186177 @default.
- W2022630006 cites W4241922517 @default.
- W2022630006 cites W4301018702 @default.
- W2022630006 doi "https://doi.org/10.1007/bf02399951" @default.
- W2022630006 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8894053" @default.
- W2022630006 hasPublicationYear "1996" @default.
- W2022630006 type Work @default.
- W2022630006 sameAs 2022630006 @default.
- W2022630006 citedByCount "92" @default.
- W2022630006 countsByYear W20226300062012 @default.
- W2022630006 countsByYear W20226300062013 @default.
- W2022630006 countsByYear W20226300062015 @default.
- W2022630006 countsByYear W20226300062016 @default.
- W2022630006 countsByYear W20226300062019 @default.
- W2022630006 countsByYear W20226300062020 @default.
- W2022630006 countsByYear W20226300062022 @default.
- W2022630006 countsByYear W20226300062023 @default.
- W2022630006 crossrefType "journal-article" @default.
- W2022630006 hasAuthorship W2022630006A5045488672 @default.
- W2022630006 hasAuthorship W2022630006A5062501052 @default.
- W2022630006 hasAuthorship W2022630006A5066564174 @default.
- W2022630006 hasAuthorship W2022630006A5085854115 @default.
- W2022630006 hasBestOaLocation W20226300062 @default.
- W2022630006 hasConcept C104317684 @default.
- W2022630006 hasConcept C141231307 @default.
- W2022630006 hasConcept C178790620 @default.
- W2022630006 hasConcept C185592680 @default.
- W2022630006 hasConcept C2777563447 @default.
- W2022630006 hasConcept C49105822 @default.
- W2022630006 hasConcept C54355233 @default.
- W2022630006 hasConcept C70721500 @default.
- W2022630006 hasConcept C86803240 @default.
- W2022630006 hasConceptScore W2022630006C104317684 @default.
- W2022630006 hasConceptScore W2022630006C141231307 @default.
- W2022630006 hasConceptScore W2022630006C178790620 @default.
- W2022630006 hasConceptScore W2022630006C185592680 @default.
- W2022630006 hasConceptScore W2022630006C2777563447 @default.
- W2022630006 hasConceptScore W2022630006C49105822 @default.
- W2022630006 hasConceptScore W2022630006C54355233 @default.
- W2022630006 hasConceptScore W2022630006C70721500 @default.
- W2022630006 hasConceptScore W2022630006C86803240 @default.
- W2022630006 hasIssue "7-8" @default.
- W2022630006 hasLocation W20226300061 @default.
- W2022630006 hasLocation W20226300062 @default.
- W2022630006 hasLocation W20226300063 @default.
- W2022630006 hasOpenAccess W2022630006 @default.
- W2022630006 hasPrimaryLocation W20226300061 @default.
- W2022630006 hasRelatedWork W1491897707 @default.
- W2022630006 hasRelatedWork W167405190 @default.
- W2022630006 hasRelatedWork W1875391864 @default.
- W2022630006 hasRelatedWork W2069409970 @default.
- W2022630006 hasRelatedWork W2089399380 @default.
- W2022630006 hasRelatedWork W2333833013 @default.
- W2022630006 hasRelatedWork W2371958667 @default.
- W2022630006 hasRelatedWork W2375570161 @default.
- W2022630006 hasRelatedWork W2987420353 @default.
- W2022630006 hasRelatedWork W3000699638 @default.
- W2022630006 hasVolume "34" @default.
- W2022630006 isParatext "false" @default.
- W2022630006 isRetracted "false" @default.
- W2022630006 magId "2022630006" @default.
- W2022630006 workType "article" @default.