Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022646503> ?p ?o ?g. }
- W2022646503 endingPage "1562" @default.
- W2022646503 startingPage "1557" @default.
- W2022646503 abstract "1 The NO donor 3-morpholino-sydnonimine (SIN-1; 0.01–10 μm) evoked concentration-dependent relaxation of rat isolated mesenteric arteries pre-constricted with phenylephrine (1–3μm). The relaxation to SIN-1 was not significantly different between endothelium-intact or denuded arterial segments or segments in which basal nitric oxide (NO) synthesis was inhibited (n = 8; P > 0.05). In contrast, the membrane permeable analogue of guanosine 3′:5′-cyclic monophosphate (cyclic GMP), 8-Br-cyclic GMP (0.01-1 mM), was much less effective in relaxing intact than denuded arterial segments or intact arterial segments pre-incubated with NO synthase blockers (n = 4;P < 0.01). 2 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-l-one (ODQ; 10 μm; 10 min) alone, did not alter SIN-1-evoked relaxation in any tissues (n = 5; P > 0.05). However, in parallel experiments, ODQ almost completely inhibited both basal and SIN-1-stimulated production of cyclic GMP in both the presence and absence of NO synthase blockers (n = 6; P < 0.01) indicating that full relaxation to SIN-1 can be achieved in the absence of an increase in cyclic GMP. 3 Exposure of endothelium-intact arterial segments to the potassium channel blocker charybdotoxin (50 nM; 10 min), significantly inhibited SIN-1-evoked relaxation, reducing the maximum response by around 90% (n = 5; P < 0.01). In contrast, in arterial segments in which either the endothelial cell layer had been removed or basal NO synthesis inhibited, relaxation to SIN-1 was not reduced in the presence of charybdotoxin (n = 6; P > 0.05). However, in the presence of NO synthase blockers and L-arginine (300 μm) together, charybdotoxin did significantly inhibit SIN-1-evoked relaxation to a similar extent as intact tissues (maximum response reduced by around 80%; n = 4; P < 0.01). 4 Pre-incubation with apamin (30 nM; 10 min) or glibenclamide (10 μm; 10 min) did not alter SIN-1-evoked relaxation of phenylephrine-induced tone in any tissues (n = 4 and n = 6, respectively; P > 0.05). However, in the presence of either ODQ and apamin, or ODQ and glibenclamide, SIN-1-evoked relaxation was significantly attenuated in intact arterial segments and segments in which NO synthesis was blocked. 5 Exposure of intact arterial segments to charybdotoxin and apamin, in the presence of NO synthase blockers, also significantly inhibited SIN-1-evoked relaxation, reducing the maximum response by around 80% (n = 4; P < 0.01). 6 Addition of superoxide dismutase (SOD; 30 u ml−1), potentiated relaxations to SIN-1 in all tissues, but did not alter the effects of charybdotoxin and ODQ on SIN-1-evoked relaxation. 7 These data show that although relaxation to the NO-donor SIN-1 is not significantly different between endothelium-intact and denuded arterial segments, the mechanisms which mediate SIN-1-evoked relaxation in the rat isolated mesenteric artery appear to be modulated by the basal release of endothelium-derived NO. In the presence of an intact endothelial cell layer, the major mechanism for SIN-1-evoked relaxation appears to be the activation of charybdotoxin-sensitive potassium channels. In contrast, when basal NO synthesis is inhibited, SIN-1 appears to cause full relaxation by both the activation of a charybdotoxin-sensitive pathway and the stimulation of soluble guanylyl cyclase." @default.
- W2022646503 created "2016-06-24" @default.
- W2022646503 creator A5034078844 @default.
- W2022646503 creator A5041533585 @default.
- W2022646503 creator A5047830721 @default.
- W2022646503 creator A5079587430 @default.
- W2022646503 date "1996-12-01" @default.
- W2022646503 modified "2023-09-26" @default.
- W2022646503 title "Evidence that potassium channels make a major contribution to SIN-1-evoked relaxation of rat isolated mesenteric artery" @default.
- W2022646503 cites W1989744696 @default.
- W2022646503 cites W2002591410 @default.
- W2022646503 cites W2012823060 @default.
- W2022646503 cites W2022646503 @default.
- W2022646503 cites W2041899253 @default.
- W2022646503 cites W2063503589 @default.
- W2022646503 cites W2070243129 @default.
- W2022646503 cites W2090968114 @default.
- W2022646503 cites W2256724151 @default.
- W2022646503 cites W2398420755 @default.
- W2022646503 cites W2405690953 @default.
- W2022646503 cites W2417406677 @default.
- W2022646503 doi "https://doi.org/10.1111/j.1476-5381.1996.tb16072.x" @default.
- W2022646503 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1915782" @default.
- W2022646503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8982501" @default.
- W2022646503 hasPublicationYear "1996" @default.
- W2022646503 type Work @default.
- W2022646503 sameAs 2022646503 @default.
- W2022646503 citedByCount "49" @default.
- W2022646503 countsByYear W20226465032012 @default.
- W2022646503 countsByYear W20226465032016 @default.
- W2022646503 countsByYear W20226465032017 @default.
- W2022646503 countsByYear W20226465032020 @default.
- W2022646503 countsByYear W20226465032021 @default.
- W2022646503 crossrefType "journal-article" @default.
- W2022646503 hasAuthorship W2022646503A5034078844 @default.
- W2022646503 hasAuthorship W2022646503A5041533585 @default.
- W2022646503 hasAuthorship W2022646503A5047830721 @default.
- W2022646503 hasAuthorship W2022646503A5079587430 @default.
- W2022646503 hasBestOaLocation W20226465032 @default.
- W2022646503 hasConcept C120770815 @default.
- W2022646503 hasConcept C126322002 @default.
- W2022646503 hasConcept C134018914 @default.
- W2022646503 hasConcept C163415756 @default.
- W2022646503 hasConcept C178790620 @default.
- W2022646503 hasConcept C185592680 @default.
- W2022646503 hasConcept C2776820930 @default.
- W2022646503 hasConcept C2776919887 @default.
- W2022646503 hasConcept C2776992346 @default.
- W2022646503 hasConcept C2777022698 @default.
- W2022646503 hasConcept C2777952589 @default.
- W2022646503 hasConcept C2778457506 @default.
- W2022646503 hasConcept C2779411790 @default.
- W2022646503 hasConcept C2780419564 @default.
- W2022646503 hasConcept C2780771799 @default.
- W2022646503 hasConcept C2781295685 @default.
- W2022646503 hasConcept C517785266 @default.
- W2022646503 hasConcept C519063684 @default.
- W2022646503 hasConcept C519581460 @default.
- W2022646503 hasConcept C71924100 @default.
- W2022646503 hasConcept C83743174 @default.
- W2022646503 hasConcept C84393581 @default.
- W2022646503 hasConceptScore W2022646503C120770815 @default.
- W2022646503 hasConceptScore W2022646503C126322002 @default.
- W2022646503 hasConceptScore W2022646503C134018914 @default.
- W2022646503 hasConceptScore W2022646503C163415756 @default.
- W2022646503 hasConceptScore W2022646503C178790620 @default.
- W2022646503 hasConceptScore W2022646503C185592680 @default.
- W2022646503 hasConceptScore W2022646503C2776820930 @default.
- W2022646503 hasConceptScore W2022646503C2776919887 @default.
- W2022646503 hasConceptScore W2022646503C2776992346 @default.
- W2022646503 hasConceptScore W2022646503C2777022698 @default.
- W2022646503 hasConceptScore W2022646503C2777952589 @default.
- W2022646503 hasConceptScore W2022646503C2778457506 @default.
- W2022646503 hasConceptScore W2022646503C2779411790 @default.
- W2022646503 hasConceptScore W2022646503C2780419564 @default.
- W2022646503 hasConceptScore W2022646503C2780771799 @default.
- W2022646503 hasConceptScore W2022646503C2781295685 @default.
- W2022646503 hasConceptScore W2022646503C517785266 @default.
- W2022646503 hasConceptScore W2022646503C519063684 @default.
- W2022646503 hasConceptScore W2022646503C519581460 @default.
- W2022646503 hasConceptScore W2022646503C71924100 @default.
- W2022646503 hasConceptScore W2022646503C83743174 @default.
- W2022646503 hasConceptScore W2022646503C84393581 @default.
- W2022646503 hasIssue "8" @default.
- W2022646503 hasLocation W20226465031 @default.
- W2022646503 hasLocation W20226465032 @default.
- W2022646503 hasLocation W20226465033 @default.
- W2022646503 hasLocation W20226465034 @default.
- W2022646503 hasOpenAccess W2022646503 @default.
- W2022646503 hasPrimaryLocation W20226465031 @default.
- W2022646503 hasRelatedWork W2001072936 @default.
- W2022646503 hasRelatedWork W2011675741 @default.
- W2022646503 hasRelatedWork W2022646503 @default.
- W2022646503 hasRelatedWork W2043076116 @default.
- W2022646503 hasRelatedWork W2046193597 @default.
- W2022646503 hasRelatedWork W2066412659 @default.
- W2022646503 hasRelatedWork W2137143358 @default.
- W2022646503 hasRelatedWork W2161661722 @default.