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• W2022650637 abstract "The three-dimensional configuration of the nuclei of the trinucleate pollen grain of Spinacia oleracea L. has been examined by means of confocal laser scanning fluorescence microscopy (CLSM). It shows the presence of a male germ unit (MGU) in which all three nuclei are usually positioned in the periphery of the pollen grain. After freeze fixation and freeze substitution, the ultrastructure is better preserved than with standard chemical fixation. It shows the presence inside the pollen grain of mitochondria, dictyosomes, large starch-containing plastids, endoplasmic reticulum (ER), vacuoles and the MGU. In the sperm cells mitochondria, vesicles, dictyosomes and ER are also found. No microtubules were found in the grain and only very few inside the sperm cells. This is in contrast with earlier published results where fluorescent-labeled antibodies were used." @default.
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• W2022650637 date "1991-08-01" @default.
• W2022650637 modified "2023-10-09" @default.
• W2022650637 title "Studies of the Mature Pollen of <i>Spinacia oleracea</i> after Freeze Substitution and Observed with Confocal Laser Scanning Fluorescence Microscopy" @default.
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• W2022650637 doi "https://doi.org/10.1111/j.1438-8677.1991.tb00237.x" @default.
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