Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022652249> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2022652249 endingPage "89" @default.
- W2022652249 startingPage "84" @default.
- W2022652249 abstract "Quinone reductase 2 (NQO2) is a broadly expressed enzyme implicated in responses to a number of compounds, including protein kinase inhibitors, resveratrol, and antimalarial drugs. NQO2 includes a flavin adenine dinucleotide (FAD) cofactor, but X-ray crystallographic analysis of human NQO2 expressed in Escherichia coli showed that electron density for the isoalloxazine ring of FAD was weak and there was no electron density for the adenine mononucleotide moiety. Reversed-phase high-performance liquid chromatography (HPLC) of the NQO2 preparation indicated that FAD was not present and only 38% of the protomers contained flavin mononucleotide (FMN), explaining the weak electron density for FAD in the crystallographic analysis. A method for purifying NQO2 and reconstituting with FAD such that the final content approaches 100% occupancy with FAD is presented here. The enzyme prepared in this manner has a high specific activity, and there is strong electron density for the FAD cofactor in the crystal structure. Analysis of NQO2 crystal structures present in the Protein Data Bank indicates that many may have sub-stoichiometric cofactor content and/or contain FMN rather than FAD. This method of purification and reconstitution will help to optimize structural and functional studies of NQO2 and possibly other flavoproteins." @default.
- W2022652249 created "2016-06-24" @default.
- W2022652249 creator A5059923951 @default.
- W2022652249 creator A5077679932 @default.
- W2022652249 creator A5086809539 @default.
- W2022652249 date "2012-01-01" @default.
- W2022652249 modified "2023-10-16" @default.
- W2022652249 title "Flavin adenine dinucleotide content of quinone reductase 2: Analysis and optimization for structure–function studies" @default.
- W2022652249 cites W196616756 @default.
- W2022652249 cites W1967532400 @default.
- W2022652249 cites W1979092710 @default.
- W2022652249 cites W1983457087 @default.
- W2022652249 cites W1986191025 @default.
- W2022652249 cites W1989096321 @default.
- W2022652249 cites W1994548752 @default.
- W2022652249 cites W2003894821 @default.
- W2022652249 cites W2016055538 @default.
- W2022652249 cites W2022582116 @default.
- W2022652249 cites W2057468615 @default.
- W2022652249 cites W2057761407 @default.
- W2022652249 cites W2058146658 @default.
- W2022652249 cites W2069207813 @default.
- W2022652249 cites W2088995397 @default.
- W2022652249 cites W2090912504 @default.
- W2022652249 cites W2098851061 @default.
- W2022652249 cites W2099937543 @default.
- W2022652249 cites W2110893691 @default.
- W2022652249 cites W2113420845 @default.
- W2022652249 cites W2150603656 @default.
- W2022652249 cites W2170869297 @default.
- W2022652249 cites W2180229411 @default.
- W2022652249 cites W25878943 @default.
- W2022652249 cites W4210675630 @default.
- W2022652249 cites W982707794 @default.
- W2022652249 doi "https://doi.org/10.1016/j.ab.2011.09.011" @default.
- W2022652249 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21971443" @default.
- W2022652249 hasPublicationYear "2012" @default.
- W2022652249 type Work @default.
- W2022652249 sameAs 2022652249 @default.
- W2022652249 citedByCount "11" @default.
- W2022652249 countsByYear W20226522492013 @default.
- W2022652249 countsByYear W20226522492014 @default.
- W2022652249 countsByYear W20226522492015 @default.
- W2022652249 countsByYear W20226522492020 @default.
- W2022652249 countsByYear W20226522492022 @default.
- W2022652249 crossrefType "journal-article" @default.
- W2022652249 hasAuthorship W2022652249A5059923951 @default.
- W2022652249 hasAuthorship W2022652249A5077679932 @default.
- W2022652249 hasAuthorship W2022652249A5086809539 @default.
- W2022652249 hasConcept C134651460 @default.
- W2022652249 hasConcept C181199279 @default.
- W2022652249 hasConcept C185592680 @default.
- W2022652249 hasConcept C197957613 @default.
- W2022652249 hasConcept C2775859393 @default.
- W2022652249 hasConcept C2777427158 @default.
- W2022652249 hasConcept C2780643102 @default.
- W2022652249 hasConcept C33093398 @default.
- W2022652249 hasConcept C55493867 @default.
- W2022652249 hasConcept C71240020 @default.
- W2022652249 hasConcept C71995715 @default.
- W2022652249 hasConceptScore W2022652249C134651460 @default.
- W2022652249 hasConceptScore W2022652249C181199279 @default.
- W2022652249 hasConceptScore W2022652249C185592680 @default.
- W2022652249 hasConceptScore W2022652249C197957613 @default.
- W2022652249 hasConceptScore W2022652249C2775859393 @default.
- W2022652249 hasConceptScore W2022652249C2777427158 @default.
- W2022652249 hasConceptScore W2022652249C2780643102 @default.
- W2022652249 hasConceptScore W2022652249C33093398 @default.
- W2022652249 hasConceptScore W2022652249C55493867 @default.
- W2022652249 hasConceptScore W2022652249C71240020 @default.
- W2022652249 hasConceptScore W2022652249C71995715 @default.
- W2022652249 hasIssue "1" @default.
- W2022652249 hasLocation W20226522491 @default.
- W2022652249 hasLocation W20226522492 @default.
- W2022652249 hasOpenAccess W2022652249 @default.
- W2022652249 hasPrimaryLocation W20226522491 @default.
- W2022652249 hasRelatedWork W1588481568 @default.
- W2022652249 hasRelatedWork W1596235536 @default.
- W2022652249 hasRelatedWork W1994854766 @default.
- W2022652249 hasRelatedWork W2032853478 @default.
- W2022652249 hasRelatedWork W2049785072 @default.
- W2022652249 hasRelatedWork W2069310822 @default.
- W2022652249 hasRelatedWork W2081785349 @default.
- W2022652249 hasRelatedWork W2134700891 @default.
- W2022652249 hasRelatedWork W3137023970 @default.
- W2022652249 hasRelatedWork W2182012805 @default.
- W2022652249 hasVolume "420" @default.
- W2022652249 isParatext "false" @default.
- W2022652249 isRetracted "false" @default.
- W2022652249 magId "2022652249" @default.
- W2022652249 workType "article" @default.