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• W2022658157 abstract "There is an antigenic glycoprotein (Mr 48 kD) present on the surfaces of erythrocytes of embryonic and young chickens that cannot be detected on the circulating erythrocytes in adult birds. This antigen, generally defined by this differential expression, has been thought to be associated with the maturation of hematopoietic tissues. We now present evidence, based on the use of a monoclonal antibody, maEE1, and the characteristic pattern of this glycoprotein on two-dimensional (2D) gels, that this antigen, which we have named chickEE, is expressed in a number of other embryonic and adult tissues. Immunofluorescent labeling of cryosections and flow-cytometric analysis of cells labeled with maEE1 have revealed the presence of chickEE in the retina (present in all layers), in muscle tissues (present in the endomysium and within the vascular endothelium), in the liver (especially evident on the lateral surface of hepatocytes and within the sinusoids), on epithelia such as the gut and kidney tubule epithelium and within lymphoid organs (present on bursacytes, splenocytes, thymocytes and peripheral leukocytes, and again within the endothelium) of young and adult animals. The 2D gel patterns of chickEE derived from embryonic tissues (retina, hind limb, thymus and bursa) and the adult tissues (retina and spleen) are very similar to that of the embryonic erythrocyte. Thus, the extended reactivity of the monoclonal antibody to chickEE, maEE1, with additional tissues is, in at least the tissues examined, based on the presence of the chickEE glycoprotein and not on incidental cross-reactivity. The evidence presented in this paper for the widely-shared expression of chickEE antigen makes it necessary to reconsider the function of this component of the cell surface." @default.
• W2022658157 created "2016-06-24" @default.
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• W2022658157 date "1986-12-01" @default.
• W2022658157 modified "2023-10-05" @default.
• W2022658157 title "Chick embryonic erythrocyte antigen—Tissues sharing expression" @default.
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• W2022658157 doi "https://doi.org/10.1016/0014-4827(86)90171-0" @default.
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