FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022673248> ?p ?o ?g. }

• W2022673248 endingPage "110" @default.
• W2022673248 startingPage "93" @default.
• W2022673248 abstract "The Eco RV DNA-(adenine-N6)-methyltransferase recognizes GATATC sequences and modifies the first adenine residue within this site. We show here, that the enzyme binds to the DNA and the cofactor S-adenosylmethionine (AdoMet) in an ordered bi-bi fashion, with AdoMet being bound first. M.Eco RV binds DNA in a non-specific manner and the enzyme searches for its recognition site by linear diffusion with a range of approximately 1800 bp. During linear diffusion the enzyme continuously scans the DNA for the presence of recognition sites. Upon specific M.Eco RV-DNA complex formation a strong increase in the fluorescence of an oligonucleotide containing a 2-aminopurine base analogue at the GAT-2AP-TC position is observed which, most likely, is correlated with DNA bending. In contrast to the GAT-2AP-TC substrate, a G-2AP-TATC substrate in which the target base is replaced by 2-aminopurine does not show an increase in fluorescence upon M.Eco RV binding, demonstrating that 2-aminopurine is not a general tool to detect base flipping. Stopped-flow experiments show that DNA bending is a fast process with rate constants>10 s−1. In the presence of cofactor, the specific complex adopts a second conformation, in which the target sequence is more tightly contacted by the enzyme. M.Eco RV exists in an open and in a closed state that are in slow equilibrium. Closing the open state is a slow process (rate constant ≈0.7 min−1) that limits the rate of DNA methylation under single turnover conditions. Product release requires opening of the closed complex which is very slow (rate constant ≈0.05–0.1 min−1) and limits the rate of DNA methylation under multiple turnover conditions. M.Eco RV methylates DNA sequences containing more than one recognition sites in a distributive manner. Since the dissociation rate from non-specific DNA does not depend on the length of the DNA fragment, DNA dissociation does not preferentially occur at the ends of the DNA." @default.
• W2022673248 created "2016-06-24" @default.
• W2022673248 creator A5020110134 @default.
• W2022673248 creator A5070638287 @default.
• W2022673248 date "2000-10-01" @default.
• W2022673248 modified "2023-09-29" @default.
• W2022673248 title "Molecular enzymology of the Eco RV DNA-(adenine-N6)-methyltransferase: kinetics of DNA binding and bending, kinetic mechanism and linear diffusion of the enzyme on DNA" @default.
• W2022673248 cites W1487309988 @default.
• W2022673248 cites W1548141954 @default.
• W2022673248 cites W1548975209 @default.
• W2022673248 cites W1557060163 @default.
• W2022673248 cites W1579716589 @default.
• W2022673248 cites W1585338920 @default.
• W2022673248 cites W1588041328 @default.
• W2022673248 cites W1597798536 @default.
• W2022673248 cites W1609597420 @default.
• W2022673248 cites W1965127482 @default.
• W2022673248 cites W1969628353 @default.
• W2022673248 cites W1969673702 @default.
• W2022673248 cites W1973011601 @default.
• W2022673248 cites W1974443604 @default.
• W2022673248 cites W1974925543 @default.
• W2022673248 cites W1979229978 @default.
• W2022673248 cites W1980622413 @default.
• W2022673248 cites W1981333260 @default.
• W2022673248 cites W1982898133 @default.
• W2022673248 cites W1985817667 @default.
• W2022673248 cites W1987420251 @default.
• W2022673248 cites W1988278521 @default.
• W2022673248 cites W1994162276 @default.
• W2022673248 cites W1997974036 @default.
• W2022673248 cites W1998691654 @default.
• W2022673248 cites W2001122405 @default.
• W2022673248 cites W2004081190 @default.
• W2022673248 cites W2006419303 @default.
• W2022673248 cites W2008243144 @default.
• W2022673248 cites W2008385451 @default.
• W2022673248 cites W2010880750 @default.
• W2022673248 cites W2011897495 @default.
• W2022673248 cites W2014283477 @default.
• W2022673248 cites W2017191738 @default.
• W2022673248 cites W2018344214 @default.
• W2022673248 cites W2018925300 @default.
• W2022673248 cites W2019367624 @default.
• W2022673248 cites W2019492821 @default.
• W2022673248 cites W2025784041 @default.
• W2022673248 cites W2028997244 @default.
• W2022673248 cites W2029104512 @default.
• W2022673248 cites W2029602153 @default.
• W2022673248 cites W2030305640 @default.
• W2022673248 cites W2033551900 @default.
• W2022673248 cites W2036082188 @default.
• W2022673248 cites W2039113975 @default.
• W2022673248 cites W2044575976 @default.
• W2022673248 cites W2046241907 @default.
• W2022673248 cites W2049210709 @default.
• W2022673248 cites W2050728905 @default.
• W2022673248 cites W2050946836 @default.
• W2022673248 cites W2053217245 @default.
• W2022673248 cites W2055479492 @default.
• W2022673248 cites W2056377405 @default.
• W2022673248 cites W2056588849 @default.
• W2022673248 cites W2060248442 @default.
• W2022673248 cites W2060446375 @default.
• W2022673248 cites W2061648185 @default.
• W2022673248 cites W2061801549 @default.
• W2022673248 cites W2062960134 @default.
• W2022673248 cites W2068219372 @default.
• W2022673248 cites W2073535845 @default.
• W2022673248 cites W2076129724 @default.
• W2022673248 cites W2077890483 @default.
• W2022673248 cites W2084015439 @default.
• W2022673248 cites W2085486557 @default.
• W2022673248 cites W2094239981 @default.
• W2022673248 cites W2101191634 @default.
• W2022673248 cites W2104587229 @default.
• W2022673248 cites W2109881276 @default.
• W2022673248 cites W2115150990 @default.
• W2022673248 cites W2126916732 @default.
• W2022673248 cites W2134326554 @default.
• W2022673248 cites W2136716105 @default.
• W2022673248 cites W2150972126 @default.
• W2022673248 cites W2156976901 @default.
• W2022673248 cites W2157574275 @default.
• W2022673248 cites W2163966395 @default.
• W2022673248 cites W2164237219 @default.
• W2022673248 cites W2179631201 @default.
• W2022673248 cites W4237365353 @default.
• W2022673248 cites W75205181 @default.
• W2022673248 doi "https://doi.org/10.1006/jmbi.2000.4127" @default.
• W2022673248 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11021972" @default.
• W2022673248 hasPublicationYear "2000" @default.
• W2022673248 type Work @default.
• W2022673248 sameAs 2022673248 @default.
• W2022673248 citedByCount "62" @default.
• W2022673248 countsByYear W20226732482012 @default.
• W2022673248 countsByYear W20226732482014 @default.
• W2022673248 countsByYear W20226732482015 @default.

• next