Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022677304> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2022677304 endingPage "253" @default.
- W2022677304 startingPage "243" @default.
- W2022677304 abstract "Holstein or Angus cows were superovulated, inseminated with fresh bull semen, and necropsied about 12 h after estimated time of ovulation. Ova were centrifuged at 15,600 G for 3 to 8 min to reveal pronuclei. In Experiment 1, pronuclear bovine embryos were transferred to ligated or unligated oviducts of 1-d pseudopregnant rabbits for 7 d; 30 of 32 embryos were recovered from ligated oviducts but only 2 of 26 from oviducts and uterine horns of unligated oviducts. In Experiment 2, a Rous sarcoma virus-chloramphenicol acetyl transferase fusion gene was injected into one pronucleus of about half of 404 fertilized bovine ova, using a micromanipulator and interference contrast optics. Injected and noninjected embryos were then transferred to opposite ligated rabbit oviducts. Embryos were recovered after 7, 8 or 9 d. Of 120 centrifuged but uninjected embryos recovered from rabbit oviducts, 66 (55%) were in the morula to hatching blastocyst stage of development. Of 105 embryos centrifuged and injected with foreign DNA, 55 (52%) were in the morula to hatching blastocyst stage. In Experiment 3, centrifuged bovine embryos, noninjected or DNA-injected, were cultured in rabbit oviducts for 7 d then transferred nonsurgically to the uterus of recipient cows. Embryos were also flushed from superovulated cows 8 d after estrus and transferred directly to recipient cows. After 7 d, the uterus of recipient cows was flushed nonsurgically to recover embryos. The proportion of transferred embryos recovered with normally elongated trophoblastic membranes and the proportion of recipient cows with developing embryos were 14 of 25 DNA-injected embryos, 5 of 8 cows; 6 of 15 centrifuged but noninjected embryos, 4 of 6 cows; and 11 of 29 embryos transferred directly, 5 of 8 cows. Results indicate that bovine embryos can be cultured in rabbit oviducts and survive after transfer to cow uteri and that injection of foreign DNA may not increase embryonic loss within the first 2 wk after injection." @default.
- W2022677304 created "2016-06-24" @default.
- W2022677304 creator A5010626580 @default.
- W2022677304 creator A5028397274 @default.
- W2022677304 creator A5031397708 @default.
- W2022677304 date "1989-08-01" @default.
- W2022677304 modified "2023-10-18" @default.
- W2022677304 title "Survival of DNA-injected cow embryos temporarily cultured in rabbit oviducts" @default.
- W2022677304 cites W1969847086 @default.
- W2022677304 cites W1978224730 @default.
- W2022677304 cites W1983232505 @default.
- W2022677304 cites W1984491015 @default.
- W2022677304 cites W2003700389 @default.
- W2022677304 cites W2030485961 @default.
- W2022677304 cites W2044613450 @default.
- W2022677304 cites W2058515838 @default.
- W2022677304 cites W2066292941 @default.
- W2022677304 cites W2084544511 @default.
- W2022677304 cites W2088135095 @default.
- W2022677304 cites W2090615247 @default.
- W2022677304 cites W2108410269 @default.
- W2022677304 cites W2160779223 @default.
- W2022677304 doi "https://doi.org/10.1016/0093-691x(89)90315-4" @default.
- W2022677304 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16726671" @default.
- W2022677304 hasPublicationYear "1989" @default.
- W2022677304 type Work @default.
- W2022677304 sameAs 2022677304 @default.
- W2022677304 citedByCount "18" @default.
- W2022677304 crossrefType "journal-article" @default.
- W2022677304 hasAuthorship W2022677304A5010626580 @default.
- W2022677304 hasAuthorship W2022677304A5028397274 @default.
- W2022677304 hasAuthorship W2022677304A5031397708 @default.
- W2022677304 hasConcept C105702510 @default.
- W2022677304 hasConcept C110758877 @default.
- W2022677304 hasConcept C134018914 @default.
- W2022677304 hasConcept C16685009 @default.
- W2022677304 hasConcept C196843134 @default.
- W2022677304 hasConcept C1998276 @default.
- W2022677304 hasConcept C2777005246 @default.
- W2022677304 hasConcept C2777129507 @default.
- W2022677304 hasConcept C2778177303 @default.
- W2022677304 hasConcept C2778227567 @default.
- W2022677304 hasConcept C2779066055 @default.
- W2022677304 hasConcept C2781087480 @default.
- W2022677304 hasConcept C28748434 @default.
- W2022677304 hasConcept C62525196 @default.
- W2022677304 hasConcept C71924100 @default.
- W2022677304 hasConcept C86803240 @default.
- W2022677304 hasConcept C87073359 @default.
- W2022677304 hasConcept C95444343 @default.
- W2022677304 hasConceptScore W2022677304C105702510 @default.
- W2022677304 hasConceptScore W2022677304C110758877 @default.
- W2022677304 hasConceptScore W2022677304C134018914 @default.
- W2022677304 hasConceptScore W2022677304C16685009 @default.
- W2022677304 hasConceptScore W2022677304C196843134 @default.
- W2022677304 hasConceptScore W2022677304C1998276 @default.
- W2022677304 hasConceptScore W2022677304C2777005246 @default.
- W2022677304 hasConceptScore W2022677304C2777129507 @default.
- W2022677304 hasConceptScore W2022677304C2778177303 @default.
- W2022677304 hasConceptScore W2022677304C2778227567 @default.
- W2022677304 hasConceptScore W2022677304C2779066055 @default.
- W2022677304 hasConceptScore W2022677304C2781087480 @default.
- W2022677304 hasConceptScore W2022677304C28748434 @default.
- W2022677304 hasConceptScore W2022677304C62525196 @default.
- W2022677304 hasConceptScore W2022677304C71924100 @default.
- W2022677304 hasConceptScore W2022677304C86803240 @default.
- W2022677304 hasConceptScore W2022677304C87073359 @default.
- W2022677304 hasConceptScore W2022677304C95444343 @default.
- W2022677304 hasIssue "2" @default.
- W2022677304 hasLocation W20226773041 @default.
- W2022677304 hasLocation W20226773042 @default.
- W2022677304 hasOpenAccess W2022677304 @default.
- W2022677304 hasPrimaryLocation W20226773041 @default.
- W2022677304 hasRelatedWork W1964427914 @default.
- W2022677304 hasRelatedWork W1967551640 @default.
- W2022677304 hasRelatedWork W1984364826 @default.
- W2022677304 hasRelatedWork W1994944575 @default.
- W2022677304 hasRelatedWork W2022677304 @default.
- W2022677304 hasRelatedWork W2060146325 @default.
- W2022677304 hasRelatedWork W2084933104 @default.
- W2022677304 hasRelatedWork W2097656009 @default.
- W2022677304 hasRelatedWork W2099535287 @default.
- W2022677304 hasRelatedWork W2119558692 @default.
- W2022677304 hasVolume "32" @default.
- W2022677304 isParatext "false" @default.
- W2022677304 isRetracted "false" @default.
- W2022677304 magId "2022677304" @default.
- W2022677304 workType "article" @default.