FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022677544> ?p ?o ?g. }

• W2022677544 endingPage "588" @default.
• W2022677544 startingPage "579" @default.
• W2022677544 abstract "Abstract The mTOR alpha4 phosphoprotein is a prolactin (PRL)‐downregulated gene product that is found in the nucleus of PRL‐dependent rat Nb2 lymphoma cells. Alpha4 lacks a nuclear localization signal (NLS) and the mechanism of its nuclear targeting is unknown. Post‐translational modification by O ‐linked β‐ N ‐acetylglucosamine ( O ‐GlcNAc) moieties has been implicated in the nuclear transport of some proteins, including transcription factor Sp1. The nucleocytoplasmic enzymes O ‐β‐ N ‐acetylglucosaminyltransferase (OGT) and O ‐β‐ N ‐acetylglucosaminidase ( O ‐GlcNAcase) adds or remove O ‐GlcNAc moieties, respectively. If O ‐GlcNac moieties contribute to the nuclear targeting of alpha4, a decrease in O ‐GlcNAcylation (e.g., by inhibition of OGT) may redistribute alpha4 to the cytosol. The present study showed that alpha4 and Sp1 were both O ‐GlcNAcylated in quiescent and PRL‐treated Nb2 cells. PRL alone or PRL + streptozotocin (STZ; an O ‐GlcNAcase inhibitor) significantly ( P ≤ 0.05) increased the O ‐GlcNAc/alpha4 ratio above that in control quiescent cells. However, PRL + alloxan (ALX; an OGT inhibitor) or ALX alone did not decrease O ‐GlcNAcylation of alpha4 below that of controls and alpha4 remained nuclear. In comparison, PRL (±ALX/STZ) greatly increased Sp1 protein levels, caused a significant decrease in the GlcNAc/Sp1 ratio ( P ≤ 0.05, n = 3) as compared to controls and partially redistributed Sp1 to the cytosol. Finally, a 50% downregulation of OGT gene expression by small interfering RNA (i.e., siOGT) partially redistributed both alpha4 and Sp1 to the cytosol. The alpha4 protein partner PP2Ac had no detectable O ‐GlcNAc moieties and its nuclear distribution was not affected by siOGT. In summary, alpha4 and Sp1 contained O ‐GlcNAc moieties, which contributed to their nuclear targeting in Nb2 cells. © 2005 Wiley‐Liss, Inc." @default.
• W2022677544 created "2016-06-24" @default.
• W2022677544 creator A5013883951 @default.
• W2022677544 creator A5076891240 @default.
• W2022677544 creator A5078717940 @default.
• W2022677544 date "2005-07-28" @default.
• W2022677544 modified "2023-10-16" @default.
• W2022677544 title "Role ofO-linked β-N-acetylglucosamine modification in the subcellular distribution of alpha4 phosphoprotein and Sp1 in rat lymphoma cells" @default.
• W2022677544 cites W1481480776 @default.
• W2022677544 cites W1513739797 @default.
• W2022677544 cites W1568866400 @default.
• W2022677544 cites W1964319777 @default.
• W2022677544 cites W1969869054 @default.
• W2022677544 cites W1969939865 @default.
• W2022677544 cites W1983850779 @default.
• W2022677544 cites W1998456952 @default.
• W2022677544 cites W1998978324 @default.
• W2022677544 cites W2000652481 @default.
• W2022677544 cites W2008580521 @default.
• W2022677544 cites W2025379444 @default.
• W2022677544 cites W2025577759 @default.
• W2022677544 cites W2026332814 @default.
• W2022677544 cites W2032500268 @default.
• W2022677544 cites W2040581741 @default.
• W2022677544 cites W2044688375 @default.
• W2022677544 cites W2047953146 @default.
• W2022677544 cites W2049961020 @default.
• W2022677544 cites W2051852493 @default.
• W2022677544 cites W2061152844 @default.
• W2022677544 cites W2062692712 @default.
• W2022677544 cites W2068693511 @default.
• W2022677544 cites W2071618706 @default.
• W2022677544 cites W2074680571 @default.
• W2022677544 cites W2094741094 @default.
• W2022677544 cites W2099449144 @default.
• W2022677544 cites W2099577277 @default.
• W2022677544 cites W2106379400 @default.
• W2022677544 cites W2108966929 @default.
• W2022677544 cites W2122849093 @default.
• W2022677544 cites W2135251652 @default.
• W2022677544 cites W2137404591 @default.
• W2022677544 cites W2137601093 @default.
• W2022677544 cites W2160494443 @default.
• W2022677544 cites W2162684571 @default.
• W2022677544 cites W2164933850 @default.
• W2022677544 cites W2416336658 @default.
• W2022677544 cites W4231786754 @default.
• W2022677544 doi "https://doi.org/10.1002/jcb.20508" @default.
• W2022677544 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16052526" @default.
• W2022677544 hasPublicationYear "2005" @default.
• W2022677544 type Work @default.
• W2022677544 sameAs 2022677544 @default.
• W2022677544 citedByCount "38" @default.
• W2022677544 countsByYear W20226775442012 @default.
• W2022677544 countsByYear W20226775442014 @default.
• W2022677544 countsByYear W20226775442015 @default.
• W2022677544 countsByYear W20226775442016 @default.
• W2022677544 countsByYear W20226775442017 @default.
• W2022677544 countsByYear W20226775442018 @default.
• W2022677544 countsByYear W20226775442020 @default.
• W2022677544 countsByYear W20226775442021 @default.
• W2022677544 countsByYear W20226775442022 @default.
• W2022677544 countsByYear W20226775442023 @default.
• W2022677544 crossrefType "journal-article" @default.
• W2022677544 hasAuthorship W2022677544A5013883951 @default.
• W2022677544 hasAuthorship W2022677544A5076891240 @default.
• W2022677544 hasAuthorship W2022677544A5078717940 @default.
• W2022677544 hasBestOaLocation W20226775441 @default.
• W2022677544 hasConcept C104317684 @default.
• W2022677544 hasConcept C11960822 @default.
• W2022677544 hasConcept C153911025 @default.
• W2022677544 hasConcept C181199279 @default.
• W2022677544 hasConcept C185592680 @default.
• W2022677544 hasConcept C190062978 @default.
• W2022677544 hasConcept C22615655 @default.
• W2022677544 hasConcept C2776879804 @default.
• W2022677544 hasConcept C2777061661 @default.
• W2022677544 hasConcept C2780114586 @default.
• W2022677544 hasConcept C29512474 @default.
• W2022677544 hasConcept C49805395 @default.
• W2022677544 hasConcept C54009773 @default.
• W2022677544 hasConcept C55493867 @default.
• W2022677544 hasConcept C59157529 @default.
• W2022677544 hasConcept C86339819 @default.
• W2022677544 hasConcept C86803240 @default.
• W2022677544 hasConcept C95444343 @default.
• W2022677544 hasConcept C98539663 @default.
• W2022677544 hasConceptScore W2022677544C104317684 @default.
• W2022677544 hasConceptScore W2022677544C11960822 @default.
• W2022677544 hasConceptScore W2022677544C153911025 @default.
• W2022677544 hasConceptScore W2022677544C181199279 @default.
• W2022677544 hasConceptScore W2022677544C185592680 @default.
• W2022677544 hasConceptScore W2022677544C190062978 @default.
• W2022677544 hasConceptScore W2022677544C22615655 @default.
• W2022677544 hasConceptScore W2022677544C2776879804 @default.
• W2022677544 hasConceptScore W2022677544C2777061661 @default.
• W2022677544 hasConceptScore W2022677544C2780114586 @default.
• W2022677544 hasConceptScore W2022677544C29512474 @default.

• next