Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022679221> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2022679221 endingPage "428" @default.
- W2022679221 startingPage "423" @default.
- W2022679221 abstract "Cytochrome b5 of human erythrocytes showed a midpoint redox potential of - 2 mV, an isoelectric point of 4.3, and a Michaelis constant of 7μ for erythrocyte cytochrome b5 reductase These values are close to those of rabbit liver cytochrome b5, though cytochrome b5 of erythrocytes is in the soluble fraction and its relative molecular mass is smaller than that of liver cytochrome b5 The concentration of cytochrome b5 in normal human erythrocytes was determined by an improved method and a value of 0.22 ± 0.02 μM was obtained. The optimum pH of the cytochrome b5 reduction by the reductase from human erythrocytes was 5.5, and about half of the maximum activity was observed at neutral pH. As electron donors, α-NADH and NADPH were 56% and 1 % as effective as β-NADH respectively. A Michaelis constant of 22 nM for β-NADH was obtained by fluorometric assay method. The rate of cytochrome b5 reduction in normal human erythrocytes was calculated to be 1.4 mM/h by the use of the substrate concentrations in vivo. The rate constant of the nonenzymatic methemoglobin reduction by reduced cytochrome b5 was determined to be 6.2 × 103 M-1 s-1. These values account for the methemoglobin reduction rate in human erythrocytes on the hypothesis proposed previously (Hultquist, Sugita), namely methemoglobin is reduced nonenzymatically by cytochrome b5 which has been reduced by NADH-cytochrome b5 reductase." @default.
- W2022679221 created "2016-06-24" @default.
- W2022679221 creator A5012679973 @default.
- W2022679221 creator A5055869109 @default.
- W2022679221 date "1979-11-01" @default.
- W2022679221 modified "2023-09-26" @default.
- W2022679221 title "Properties of Cytochrome b5, and Methemoglobin Reduction in Human Erythrocytes" @default.
- W2022679221 cites W103181843 @default.
- W2022679221 cites W1488341132 @default.
- W2022679221 cites W1523619076 @default.
- W2022679221 cites W1588946050 @default.
- W2022679221 cites W1625660792 @default.
- W2022679221 cites W165227044 @default.
- W2022679221 cites W1715196076 @default.
- W2022679221 cites W1993678594 @default.
- W2022679221 cites W2008161054 @default.
- W2022679221 cites W2043915642 @default.
- W2022679221 cites W2059360988 @default.
- W2022679221 cites W2059628610 @default.
- W2022679221 cites W2083008862 @default.
- W2022679221 cites W2120944108 @default.
- W2022679221 cites W2415213086 @default.
- W2022679221 cites W40268261 @default.
- W2022679221 cites W964245812 @default.
- W2022679221 doi "https://doi.org/10.1111/j.1432-1033.1979.tb19735.x" @default.
- W2022679221 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/520306" @default.
- W2022679221 hasPublicationYear "1979" @default.
- W2022679221 type Work @default.
- W2022679221 sameAs 2022679221 @default.
- W2022679221 citedByCount "45" @default.
- W2022679221 countsByYear W20226792212012 @default.
- W2022679221 countsByYear W20226792212013 @default.
- W2022679221 countsByYear W20226792212014 @default.
- W2022679221 countsByYear W20226792212015 @default.
- W2022679221 countsByYear W20226792212016 @default.
- W2022679221 countsByYear W20226792212017 @default.
- W2022679221 countsByYear W20226792212018 @default.
- W2022679221 countsByYear W20226792212019 @default.
- W2022679221 countsByYear W20226792212020 @default.
- W2022679221 countsByYear W20226792212022 @default.
- W2022679221 countsByYear W20226792212023 @default.
- W2022679221 crossrefType "journal-article" @default.
- W2022679221 hasAuthorship W2022679221A5012679973 @default.
- W2022679221 hasAuthorship W2022679221A5055869109 @default.
- W2022679221 hasConcept C123249941 @default.
- W2022679221 hasConcept C134651460 @default.
- W2022679221 hasConcept C14471203 @default.
- W2022679221 hasConcept C171305022 @default.
- W2022679221 hasConcept C181199279 @default.
- W2022679221 hasConcept C185592680 @default.
- W2022679221 hasConcept C20705724 @default.
- W2022679221 hasConcept C26865036 @default.
- W2022679221 hasConcept C2776217839 @default.
- W2022679221 hasConcept C2778917026 @default.
- W2022679221 hasConcept C2779384962 @default.
- W2022679221 hasConcept C2780768313 @default.
- W2022679221 hasConcept C28859421 @default.
- W2022679221 hasConcept C29311851 @default.
- W2022679221 hasConcept C55493867 @default.
- W2022679221 hasConceptScore W2022679221C123249941 @default.
- W2022679221 hasConceptScore W2022679221C134651460 @default.
- W2022679221 hasConceptScore W2022679221C14471203 @default.
- W2022679221 hasConceptScore W2022679221C171305022 @default.
- W2022679221 hasConceptScore W2022679221C181199279 @default.
- W2022679221 hasConceptScore W2022679221C185592680 @default.
- W2022679221 hasConceptScore W2022679221C20705724 @default.
- W2022679221 hasConceptScore W2022679221C26865036 @default.
- W2022679221 hasConceptScore W2022679221C2776217839 @default.
- W2022679221 hasConceptScore W2022679221C2778917026 @default.
- W2022679221 hasConceptScore W2022679221C2779384962 @default.
- W2022679221 hasConceptScore W2022679221C2780768313 @default.
- W2022679221 hasConceptScore W2022679221C28859421 @default.
- W2022679221 hasConceptScore W2022679221C29311851 @default.
- W2022679221 hasConceptScore W2022679221C55493867 @default.
- W2022679221 hasIssue "2" @default.
- W2022679221 hasLocation W20226792211 @default.
- W2022679221 hasLocation W20226792212 @default.
- W2022679221 hasOpenAccess W2022679221 @default.
- W2022679221 hasPrimaryLocation W20226792211 @default.
- W2022679221 hasRelatedWork W1031849976 @default.
- W2022679221 hasRelatedWork W1987652114 @default.
- W2022679221 hasRelatedWork W2005996021 @default.
- W2022679221 hasRelatedWork W2008979971 @default.
- W2022679221 hasRelatedWork W2013524466 @default.
- W2022679221 hasRelatedWork W2033205502 @default.
- W2022679221 hasRelatedWork W2060620844 @default.
- W2022679221 hasRelatedWork W2074570464 @default.
- W2022679221 hasRelatedWork W2087467041 @default.
- W2022679221 hasRelatedWork W2316015129 @default.
- W2022679221 hasVolume "101" @default.
- W2022679221 isParatext "false" @default.
- W2022679221 isRetracted "false" @default.
- W2022679221 magId "2022679221" @default.
- W2022679221 workType "article" @default.