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• W2022679632 abstract "The need for quantitative intracellular metabolome information is central to modern applied biotechnology and systems biology. In most cases, sample preparation and metabolite analysis result in degradation of metabolites and signal suppression due to metabolite instability and matrix effects during LC–MS analysis. Therefore the application of uniformly (U) 13C-labeled cell extract as an internal standard has gained interest in recent years. In this study a multiple-step protocol has been developed for efficient preparation of U-13C-labeled Escherichia coli cell extracts in stirred-tank bioreactors on a milliliter scale with a minimal supply of costly 13C-labeled substrate. Significant reduction of fermentation medium salt concentration in the U-13C-labeled cell extract was achieved to reduce ion-suppression effects during mass-spectrometric analysis. Additionally, variation of reaction conditions in parallel-operated stirred-tank bioreactors on a milliliter scale enables the simultaneous preparation of U-13C-labeled cell extracts with varying metabolite concentrations, which is shown by an example of the labeled phosphoenolpyruvate level in E. coli." @default.
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• W2022679632 date "2015-06-01" @default.
• W2022679632 modified "2023-10-17" @default.
• W2022679632 title "Parallelized small-scale production of uniformly 13C-labeled cell extract for quantitative metabolome analysis" @default.
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• W2022679632 doi "https://doi.org/10.1016/j.ab.2015.03.008" @default.
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