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• W2022683236 abstract "Triticum monococcum represents an important source of useful genes and alleles that it would be desirable to use in wheat breeding programmes. The well-defined landmarks on the A m chromosomes could accelerate the targeted introgression of T. monococcum chromatin into the wheat genome.Fluorescence in situ hybridization (FISH) using the repetitive DNA probes pSc119.2, Afa family and pTa71 showed that the pSc119.2 probe was not suitable for the identification of A m chromosomes. In contrast, the whole set of A m chromosomes (especially chromosomes 1, 4, 5 and 7) could be discriminated based on the hybridization pattern of pTa71 and Afa family. In situ hybridization with microsatellite motifs (GAA, CAG, AAC and AGG) proved that SSRs represent additional landmarks for the identification of A m chromosomes. The most promising SSR probes were the GAA and CAG motifs, which clearly discriminated the 6A m chromosome and, when used in combination with the Afa family and pTa71 probes, allowed the whole set of A m chromosomes to be reliably identified.In conclusion, fluorescence in situ hybridization using the repetitive DNA probes Afa family and pTa71, combined with SSR probes, makes it possible to identify the A m chromosomes of T. monococcum and to discriminate them from A u chromosomes in the polyploid wheat background." @default.
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• W2022683236 date "2012-06-01" @default.
• W2022683236 modified "2023-10-16" @default.
• W2022683236 title "Karyotypic analysis of Triticum monococcum using standard repetitive DNA probes and simple sequence repeats" @default.
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• W2022683236 doi "https://doi.org/10.1556/aagr.60.2012.2.1" @default.
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