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• W2022684844 endingPage "10689" @default.
• W2022684844 startingPage "10678" @default.
• W2022684844 abstract "ABSTRACT Human metapneumovirus (hMPV), a recently described paramyxovirus, is a major etiological agent for lower respiratory tract disease in young children that can manifest with severe cough, bronchiolitis, and pneumonia. The hMPV fusion glycoprotein (F) shares conserved functional domains with other paramyxovirus F proteins that are important for virus entry and spread. For other paramyxovirus F proteins, cleavage of a precursor protein (F 0 ) into F 1 and F 2 exposes a fusion peptide at the N terminus of the F 1 fragment, a likely prerequisite for fusion activity. Many hMPV strains have been reported to require trypsin for growth in tissue culture. The majority of these strains contain RQSR at the putative cleavage site. However, strains hMPV/NL/1/00 and hMPV/NL/1/99 expanded in our laboratory contain the sequence RQPR and do not require trypsin for growth in Vero cells. The contribution of this single amino acid change was verified directly by generating recombinant virus (rhMPV/NL/1/00) with either proline or serine at position 101 in F. These results suggested that cleavage of F protein in Vero cells could be achieved by trypsin or S101P amino acid substitution in the putative cleavage site motif. Moreover, trypsin-independent cleavage of hMPV F containing 101P was enhanced by the amino acid substitution E93K. In hamsters, rhMPV/93K/101S and rhMPV/93K/101P grew to equivalent titers in the respiratory tract and replication was restricted to respiratory tissues. The ability of these hMPV strains to replicate efficiently in the absence of trypsin should greatly facilitate the generation, preclinical testing, and manufacturing of attenuated hMPV vaccine candidates." @default.
• W2022684844 created "2016-06-24" @default.
• W2022684844 creator A5029884063 @default.
• W2022684844 creator A5033259140 @default.
• W2022684844 creator A5041726253 @default.
• W2022684844 creator A5057464778 @default.
• W2022684844 creator A5086359492 @default.
• W2022684844 date "2005-08-15" @default.
• W2022684844 modified "2023-09-25" @default.
• W2022684844 title "An S101P Substitution in the Putative Cleavage Motif of the Human Metapneumovirus Fusion Protein Is a Major Determinant for Trypsin-Independent Growth in Vero Cells and Does Not Alter Tissue Tropism in Hamsters" @default.
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• W2022684844 doi "https://doi.org/10.1128/jvi.79.16.10678-10689.2005" @default.
• W2022684844 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1182652" @default.
• W2022684844 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16051860" @default.
• W2022684844 hasPublicationYear "2005" @default.
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