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- W2022685140 abstract "This paper describes a method for non-radioactive in situ hybridization providing subcellular localization of mRNA in 3 μm cryosections. We used two alternative colorimetric reactions to detect digoxigenin-labeled cRNA probes: alkaline phosphatase and HRP (horseradish peroxidase). With some probes the signal with the alkaline phosphatase reaction was intense, and diffusion of the reaction product was noticeable. Using HRP-conjugated antibodies improved the resolution but decreased the sensitivity of the signal. Photoamplification of the HRP reaction product increased the contrast and improved the sensitivity of the technique." @default.
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- W2022685140 title "Subcellular localization of α-tubulin and opsin mRNA in the goldfish retina using digoxigenin-labeled cRNA probes detected by alkaline phosphatase and HRP histochemistry" @default.
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- W2022685140 doi "https://doi.org/10.1016/0165-0270(93)90002-9" @default.
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