FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022687541> ?p ?o ?g. }

• W2022687541 endingPage "272" @default.
• W2022687541 startingPage "265" @default.
• W2022687541 abstract "The aim of this study was to identify and to characterize a highly active anti-HIV ribozyme. Therefore, the genome of HIV-1 IIIb was screened for not yet addressed GUC triplets within highly conserved sequences. Here we report the in vitro characteristics and the antiviral activity of the fittest identified anti-HIV hammerhead ribozyme, targeting the 13th GUC triplet within the HIV-1 pol gene (HHPol13). Multiple turnover kinetics were determined in vitro and revealed very promising kinetic data: Vmax = 39 nM/minute, Km = 576 nM, kcat = 3.9/minute, and Kcat/Km = 6.8/minute/μM. To analyze its antiviral activity the hammerhead ribozyme was expressed retrovirally in Hut78 cells followed by HIV-1 infection. The newly identified ribozyme conferred a long-term inhibition of HIV-1 replication until the end of the observation period at day 56. We were able to demonstrate that the antiviral activity was mainly due to a ribozyme effect combined with a limited antisense activity. Additionally, the effect of the identified ribozyme was compared with a retrovirally expressed siRNA directed against the same target in the HIV-1 pol gene. This siRNA (siPol13) showed no inhibition of HIV replication. In summary, the hammerhead ribozyme HHPol13 was demonstrated to confer superior cleavage and antiviral activity against HIV-1. These results suggest that even in the RNAi era ribozymes still have the potential as highly active antiviral agents." @default.
• W2022687541 created "2016-06-24" @default.
• W2022687541 creator A5060565941 @default.
• W2022687541 creator A5079817594 @default.
• W2022687541 creator A5080957437 @default.
• W2022687541 creator A5090795046 @default.
• W2022687541 creator A5091503909 @default.
• W2022687541 date "2009-09-01" @default.
• W2022687541 modified "2023-09-26" @default.
• W2022687541 title "Identification and Characterization of a Highly Efficient Anti-HIV Pol Hammerhead Ribozyme" @default.
• W2022687541 cites W1963640296 @default.
• W2022687541 cites W1981012370 @default.
• W2022687541 cites W1996443011 @default.
• W2022687541 cites W2010785347 @default.
• W2022687541 cites W2013961961 @default.
• W2022687541 cites W2021985794 @default.
• W2022687541 cites W2024568805 @default.
• W2022687541 cites W2026595768 @default.
• W2022687541 cites W2030621748 @default.
• W2022687541 cites W2035868736 @default.
• W2022687541 cites W2039326418 @default.
• W2022687541 cites W2041375942 @default.
• W2022687541 cites W2049519942 @default.
• W2022687541 cites W2052719140 @default.
• W2022687541 cites W2058498574 @default.
• W2022687541 cites W2141157874 @default.
• W2022687541 cites W2324063350 @default.
• W2022687541 cites W2334814433 @default.
• W2022687541 cites W4237567990 @default.
• W2022687541 doi "https://doi.org/10.1089/oli.2008.0150" @default.
• W2022687541 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19732019" @default.
• W2022687541 hasPublicationYear "2009" @default.
• W2022687541 type Work @default.
• W2022687541 sameAs 2022687541 @default.
• W2022687541 citedByCount "12" @default.
• W2022687541 countsByYear W20226875412014 @default.
• W2022687541 countsByYear W20226875412015 @default.
• W2022687541 countsByYear W20226875412019 @default.
• W2022687541 countsByYear W20226875412021 @default.
• W2022687541 countsByYear W20226875412022 @default.
• W2022687541 crossrefType "journal-article" @default.
• W2022687541 hasAuthorship W2022687541A5060565941 @default.
• W2022687541 hasAuthorship W2022687541A5079817594 @default.
• W2022687541 hasAuthorship W2022687541A5080957437 @default.
• W2022687541 hasAuthorship W2022687541A5090795046 @default.
• W2022687541 hasAuthorship W2022687541A5091503909 @default.
• W2022687541 hasConcept C104317684 @default.
• W2022687541 hasConcept C107745601 @default.
• W2022687541 hasConcept C1342335 @default.
• W2022687541 hasConcept C140704245 @default.
• W2022687541 hasConcept C153911025 @default.
• W2022687541 hasConcept C159047783 @default.
• W2022687541 hasConcept C181199279 @default.
• W2022687541 hasConcept C2522874641 @default.
• W2022687541 hasConcept C2779792011 @default.
• W2022687541 hasConcept C30951146 @default.
• W2022687541 hasConcept C41183919 @default.
• W2022687541 hasConcept C54355233 @default.
• W2022687541 hasConcept C55493867 @default.
• W2022687541 hasConcept C56856141 @default.
• W2022687541 hasConcept C66144319 @default.
• W2022687541 hasConcept C67705224 @default.
• W2022687541 hasConcept C76346623 @default.
• W2022687541 hasConcept C86803240 @default.
• W2022687541 hasConceptScore W2022687541C104317684 @default.
• W2022687541 hasConceptScore W2022687541C107745601 @default.
• W2022687541 hasConceptScore W2022687541C1342335 @default.
• W2022687541 hasConceptScore W2022687541C140704245 @default.
• W2022687541 hasConceptScore W2022687541C153911025 @default.
• W2022687541 hasConceptScore W2022687541C159047783 @default.
• W2022687541 hasConceptScore W2022687541C181199279 @default.
• W2022687541 hasConceptScore W2022687541C2522874641 @default.
• W2022687541 hasConceptScore W2022687541C2779792011 @default.
• W2022687541 hasConceptScore W2022687541C30951146 @default.
• W2022687541 hasConceptScore W2022687541C41183919 @default.
• W2022687541 hasConceptScore W2022687541C54355233 @default.
• W2022687541 hasConceptScore W2022687541C55493867 @default.
• W2022687541 hasConceptScore W2022687541C56856141 @default.
• W2022687541 hasConceptScore W2022687541C66144319 @default.
• W2022687541 hasConceptScore W2022687541C67705224 @default.
• W2022687541 hasConceptScore W2022687541C76346623 @default.
• W2022687541 hasConceptScore W2022687541C86803240 @default.
• W2022687541 hasIssue "3" @default.
• W2022687541 hasLocation W20226875411 @default.
• W2022687541 hasLocation W20226875412 @default.
• W2022687541 hasOpenAccess W2022687541 @default.
• W2022687541 hasPrimaryLocation W20226875411 @default.
• W2022687541 hasRelatedWork W2006681738 @default.
• W2022687541 hasRelatedWork W2085851730 @default.
• W2022687541 hasRelatedWork W2105440174 @default.
• W2022687541 hasRelatedWork W2135536835 @default.
• W2022687541 hasRelatedWork W2141949998 @default.
• W2022687541 hasRelatedWork W2162162459 @default.
• W2022687541 hasRelatedWork W2321027494 @default.
• W2022687541 hasRelatedWork W2323268835 @default.
• W2022687541 hasRelatedWork W2386907636 @default.
• W2022687541 hasRelatedWork W2492191741 @default.
• W2022687541 hasVolume "19" @default.

• next