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- W2022694152 abstract "독도에 자생하고 있는 6 종류의 식물뿌리로부터 내생균을 분리하였다. 동도에서 갯제비쑥, 명아주, 까마중과 서도에서 도깨비쇠고비, 술패랭이 그리고 번행초를 연구재료로 사용하였다. 총 32 종의 내생균을 분리하였고, universal primers ITS1과 ITS4를 사용하여 ITS 영역을 PCR로 증폭하여 동정하였다. 염기서열 분석결과, 6 종류의 식물의 뿌리에서 모두 32종류의 다양한 내생균류를 분리 및 동정 할 수 있었다. 독도 자생식물인 갯제비쑥 4종, 명아주 8종, 까마중 3종, 도깨비쇠고비 3종, 술패랭이 3종, 번행초 11종의 내생균이 분리되었다. 내생균들의 배양여액은 식물생장촉진활성을 확인하기 위하여 난장이벼의 유묘에 처리되었다. 그 결과, 명아주에서 분리된 Ca-5-2-2 균주가 우수하게 식물생장촉진활성을 나타내었다. 그리고 독도에 자생하는 6종류의 식물에서 Penicillium sp.와 Fusarium sp. 그리고 Aspergillus sp.의 내생균이 가장 많이 존재함을 알 수 있었다. 【Endophytic fungal strains were isolated from the roots of six species plants in the Dokdo islands. Native plant samples, such as Artemisia japonica, Chenopodium album and Solanum nigrum were isolated from Dongdo, and those such as Cyrtomium falcatum, Dianthus longicalyx and Tetragonia tetragonoides were isolated from Seodo. In total, thirty two fungal strains were isolated from these native plants. To identify the fungal strains, polymerase chain reaction (PCR) amplification of internal transcribed spacer (ITS: containing ITS1, 5.8s and ITS2 region) regions was done with universal primers ITS1 and ITS4. Endophytic fungi of four species were isolated from A. japonica, eight species from C. album, three species from S. nigrum, three species from C. falcatum, three species from D. longicalyx and eleven species from T. tetragonoides. Culture filtrates (CF) of isolated endophytic fungi were used to treatwaito-c rice seedlings to test plant growth-promoting activity. As a result of bioassay, Ca-5-2-2 strain isolated from C. album expressed highest plant growth-promotion activity. Of all the endophytic fungi isolated, Penicillium sp., Fusarium sp. and Aspergillus sp. were the most abundantly distributed fungal strains in the six plants used in this study.】" @default.
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- W2022694152 date "2011-11-30" @default.
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- W2022694152 title "Plant Growth-Promoting Activity of Endophytic Fungi Isolated from the Roots of Native Plants in Dokdo Islands" @default.
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