FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022697872> ?p ?o ?g. }

• W2022697872 endingPage "198" @default.
• W2022697872 startingPage "191" @default.
• W2022697872 abstract "Xylose reductase (XR) is the first enzyme in d-xylose metabolism, catalyzing the reduction of d-xylose to xylitol. Formation of XR in the yeast Candida tropicalis is significantly repressed in cells grown on medium that contains glucose as carbon and energy source, because of the repressive effect of glucose. This is one reason why glucose is not a suitable co-substrate for cell growth in industrial xylitol production. XR from the ascomycete Neurospora crassa (NcXR) has high catalytic efficiency; however, NcXR is not expressed in C. tropicalis because of difference in codon usage between the two species. In this study, NcXR codons were changed to those preferred in C. tropicalis. This codon-optimized NcXR gene (termed NXRG) was placed under control of a constitutive glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter derived from C. tropicalis, and integrated into the genome of xylitol dehydrogenase gene (XYL2)-disrupted C. tropicalis. High expression level of NXRG was confirmed by determining XR activity in cells grown on glucose medium. The resulting recombinant strain, LNG2, showed high XR activity (2.86 U (mg of protein)−1), whereas parent strain BSXDH-3 showed no activity. In xylitol fermentation using glucose as a co-substrate with xylose, LNG2 showed xylitol production rate 1.44 g L−1 h−1 and xylitol yield of 96% at 44 h, which were 73 and 62%, respectively, higher than corresponding values for BSXDH-3 (rate 0.83 g L−1 h−1; yield 59%)." @default.
• W2022697872 created "2016-06-24" @default.
• W2022697872 creator A5025777654 @default.
• W2022697872 creator A5044713433 @default.
• W2022697872 creator A5051673058 @default.
• W2022697872 creator A5053839131 @default.
• W2022697872 creator A5080438574 @default.
• W2022697872 date "2011-09-16" @default.
• W2022697872 modified "2023-10-18" @default.
• W2022697872 title "Xylitol production is increased by expression of codon-optimized Neurospora crassa xylose reductase gene in Candida tropicalis" @default.
• W2022697872 cites W121179188 @default.
• W2022697872 cites W1965713802 @default.
• W2022697872 cites W1965750118 @default.
• W2022697872 cites W1966749716 @default.
• W2022697872 cites W1987208367 @default.
• W2022697872 cites W1992672691 @default.
• W2022697872 cites W1995334907 @default.
• W2022697872 cites W2016396353 @default.
• W2022697872 cites W2023206399 @default.
• W2022697872 cites W2023508966 @default.
• W2022697872 cites W2032855883 @default.
• W2022697872 cites W2045858689 @default.
• W2022697872 cites W2050107614 @default.
• W2022697872 cites W2054857787 @default.
• W2022697872 cites W2061459002 @default.
• W2022697872 cites W2069843180 @default.
• W2022697872 cites W2075511930 @default.
• W2022697872 cites W2078304878 @default.
• W2022697872 cites W2078707530 @default.
• W2022697872 cites W2080438729 @default.
• W2022697872 cites W2084207316 @default.
• W2022697872 cites W2089320617 @default.
• W2022697872 cites W2118567854 @default.
• W2022697872 cites W2160530532 @default.
• W2022697872 cites W2161656422 @default.
• W2022697872 doi "https://doi.org/10.1007/s00449-011-0618-8" @default.
• W2022697872 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3250611" @default.
• W2022697872 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21922311" @default.
• W2022697872 hasPublicationYear "2011" @default.
• W2022697872 type Work @default.
• W2022697872 sameAs 2022697872 @default.
• W2022697872 citedByCount "57" @default.
• W2022697872 countsByYear W20226978722012 @default.
• W2022697872 countsByYear W20226978722013 @default.
• W2022697872 countsByYear W20226978722014 @default.
• W2022697872 countsByYear W20226978722015 @default.
• W2022697872 countsByYear W20226978722016 @default.
• W2022697872 countsByYear W20226978722017 @default.
• W2022697872 countsByYear W20226978722018 @default.
• W2022697872 countsByYear W20226978722019 @default.
• W2022697872 countsByYear W20226978722020 @default.
• W2022697872 countsByYear W20226978722021 @default.
• W2022697872 countsByYear W20226978722022 @default.
• W2022697872 countsByYear W20226978722023 @default.
• W2022697872 crossrefType "journal-article" @default.
• W2022697872 hasAuthorship W2022697872A5025777654 @default.
• W2022697872 hasAuthorship W2022697872A5044713433 @default.
• W2022697872 hasAuthorship W2022697872A5051673058 @default.
• W2022697872 hasAuthorship W2022697872A5053839131 @default.
• W2022697872 hasAuthorship W2022697872A5080438574 @default.
• W2022697872 hasBestOaLocation W20226978721 @default.
• W2022697872 hasConcept C100544194 @default.
• W2022697872 hasConcept C104317684 @default.
• W2022697872 hasConcept C143065580 @default.
• W2022697872 hasConcept C181199279 @default.
• W2022697872 hasConcept C185592680 @default.
• W2022697872 hasConcept C2775835631 @default.
• W2022697872 hasConcept C2776031079 @default.
• W2022697872 hasConcept C2776317432 @default.
• W2022697872 hasConcept C2776739539 @default.
• W2022697872 hasConcept C2779222958 @default.
• W2022697872 hasConcept C2780294150 @default.
• W2022697872 hasConcept C2781135521 @default.
• W2022697872 hasConcept C55493867 @default.
• W2022697872 hasConcept C86803240 @default.
• W2022697872 hasConceptScore W2022697872C100544194 @default.
• W2022697872 hasConceptScore W2022697872C104317684 @default.
• W2022697872 hasConceptScore W2022697872C143065580 @default.
• W2022697872 hasConceptScore W2022697872C181199279 @default.
• W2022697872 hasConceptScore W2022697872C185592680 @default.
• W2022697872 hasConceptScore W2022697872C2775835631 @default.
• W2022697872 hasConceptScore W2022697872C2776031079 @default.
• W2022697872 hasConceptScore W2022697872C2776317432 @default.
• W2022697872 hasConceptScore W2022697872C2776739539 @default.
• W2022697872 hasConceptScore W2022697872C2779222958 @default.
• W2022697872 hasConceptScore W2022697872C2780294150 @default.
• W2022697872 hasConceptScore W2022697872C2781135521 @default.
• W2022697872 hasConceptScore W2022697872C55493867 @default.
• W2022697872 hasConceptScore W2022697872C86803240 @default.
• W2022697872 hasIssue "1-2" @default.
• W2022697872 hasLocation W20226978721 @default.
• W2022697872 hasLocation W20226978722 @default.
• W2022697872 hasLocation W20226978723 @default.
• W2022697872 hasLocation W20226978724 @default.
• W2022697872 hasOpenAccess W2022697872 @default.
• W2022697872 hasPrimaryLocation W20226978721 @default.
• W2022697872 hasRelatedWork W1968011153 @default.
• W2022697872 hasRelatedWork W1992672691 @default.

• next