FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022698551> ?p ?o ?g. }

• W2022698551 abstract "Expression of some X-chromosome linked genes has recently been shown to be altered in bovine somatic cell nuclear transfer (SCNT) derived embryos (Wrenzycki et al. 2002 Biol. Reprod. 66, 127), implying that the regulatory mechanisms of X-linked transcription are affected by embryo in vitro production (IVP) methods. We analyzed the transcriptional pattern of X-linked genes (BIRC4, GAB3, HPRT1, MECP2, RPS4X, SLC25A6, and XIST) in bovine in vitro fertilized (IVF) and SCNT male and female blastocysts to determine X-inactivation status and changes resulting from IVP. We collected pools of male (n = 5 pools) and female (n = 3 pools) IVF-derived blastocysts (Bousquet et al. 1999 Theriogenology 51, 59) and male (n = 5 pools) and female (n = 3 pools) SCNT-derived blastocysts (Mastromonaco et al. 2004 Reprod. Domest. Anim. 39, 462). Each pool consisted of five blastocysts. Embryos were washed in phosphate buffered saline (PBS) + 0.1% polyvinyl alcohol (PVA), collected, and stored at -80�C. Total RNA was extracted with an Absolutely RNA Microprep kit (Stratagene, La Jolla, CA, USA), DNase I treated, and precipitated with isopropanol and linear acrylamide (Ambion, Inc., Austin, TX, USA) as a carrier. Reverse transcription was performed with Oligo-dT (Invitrogen, Burlington, Ontario, Canada) and Superscript II RT (Invitrogen). Transcript quantification was performed by quantitative real-time PCR using SYBR Green I (LightCycler system, Roche, Diagnostics, Laval, Quebec, Canada). Data analysis was performed with SAS (SAS Institute, Inc., Cary, SC, USA) using a mixed-model factorial ANOVA and with results presented as estimates of the median, ratios of estimates, and 95% confidence intervals with � = 0.05. IVF-derived male and female blastocysts possessed similar levels of the transcripts analyzed, suggesting successful dosage compensation at this developmental stage for embryos fertilized in vitro. XIST was not detected in male IVF embryos. GAB3 was not detected in any of the female groups and, in addition, HPRT1 transcripts were not detected in SCNT derived female embryos. Male and female SCNT-derived blastocysts possessed marked differences in their transcript levels, with males showing statistically significantly higher levels of BIRC4 and RPS4X and females possessing higher levels of MECP2 and SLC25A6 transcripts although differences between the latter two were not statistically significant. XIST was detected in both male and female SCNT blastocysts. We conclude that dosage compensation between male and female IVF blastocysts is achieved at this developmental stage for the transcripts examined. However, this pattern was markedly changed in the SCNT group, affecting especially female SCNT blastocysts, suggesting that the regulatory mechanisms of X-inactivation and X-linked gene expression are substantially altered in SCNT embryos probably due to aberrant epigenetic patterns and faulty genome reprogramming. We are currently analyzing X-linked transcription in male and female in vivo-derived blastocysts in order to compare this group with IVP-derived embryos. This work was funded by NSERC, CIHR, and CRC." @default.
• W2022698551 created "2016-06-24" @default.
• W2022698551 creator A5025907006 @default.
• W2022698551 creator A5051710024 @default.
• W2022698551 creator A5072878961 @default.
• W2022698551 creator A5080978757 @default.
• W2022698551 date "2006-01-01" @default.
• W2022698551 modified "2023-10-14" @default.
• W2022698551 title "64 X-LINKED GENE EXPRESSION IN BOVINE (BOS TAURUS) MALE AND FEMALE IN VITRO- FERTILIZED AND SOMATIC CELL NUCLEAR TRANSFER-DERIVED BLASTOCYSTS" @default.
• W2022698551 doi "https://doi.org/10.1071/rdv18n2ab64" @default.
• W2022698551 hasPublicationYear "2006" @default.
• W2022698551 type Work @default.
• W2022698551 sameAs 2022698551 @default.
• W2022698551 citedByCount "0" @default.
• W2022698551 crossrefType "journal-article" @default.
• W2022698551 hasAuthorship W2022698551A5025907006 @default.
• W2022698551 hasAuthorship W2022698551A5051710024 @default.
• W2022698551 hasAuthorship W2022698551A5072878961 @default.
• W2022698551 hasAuthorship W2022698551A5080978757 @default.
• W2022698551 hasConcept C104317684 @default.
• W2022698551 hasConcept C134305767 @default.
• W2022698551 hasConcept C14814345 @default.
• W2022698551 hasConcept C150194340 @default.
• W2022698551 hasConcept C153911025 @default.
• W2022698551 hasConcept C16685009 @default.
• W2022698551 hasConcept C179933525 @default.
• W2022698551 hasConcept C196843134 @default.
• W2022698551 hasConcept C202438428 @default.
• W2022698551 hasConcept C2778177303 @default.
• W2022698551 hasConcept C2779335785 @default.
• W2022698551 hasConcept C2994278967 @default.
• W2022698551 hasConcept C54355233 @default.
• W2022698551 hasConcept C71924100 @default.
• W2022698551 hasConcept C73247094 @default.
• W2022698551 hasConcept C86803240 @default.
• W2022698551 hasConcept C87073359 @default.
• W2022698551 hasConceptScore W2022698551C104317684 @default.
• W2022698551 hasConceptScore W2022698551C134305767 @default.
• W2022698551 hasConceptScore W2022698551C14814345 @default.
• W2022698551 hasConceptScore W2022698551C150194340 @default.
• W2022698551 hasConceptScore W2022698551C153911025 @default.
• W2022698551 hasConceptScore W2022698551C16685009 @default.
• W2022698551 hasConceptScore W2022698551C179933525 @default.
• W2022698551 hasConceptScore W2022698551C196843134 @default.
• W2022698551 hasConceptScore W2022698551C202438428 @default.
• W2022698551 hasConceptScore W2022698551C2778177303 @default.
• W2022698551 hasConceptScore W2022698551C2779335785 @default.
• W2022698551 hasConceptScore W2022698551C2994278967 @default.
• W2022698551 hasConceptScore W2022698551C54355233 @default.
• W2022698551 hasConceptScore W2022698551C71924100 @default.
• W2022698551 hasConceptScore W2022698551C73247094 @default.
• W2022698551 hasConceptScore W2022698551C86803240 @default.
• W2022698551 hasConceptScore W2022698551C87073359 @default.
• W2022698551 hasLocation W20226985511 @default.
• W2022698551 hasOpenAccess W2022698551 @default.
• W2022698551 hasPrimaryLocation W20226985511 @default.
• W2022698551 hasRelatedWork W1567668280 @default.
• W2022698551 hasRelatedWork W1965200776 @default.
• W2022698551 hasRelatedWork W2004306661 @default.
• W2022698551 hasRelatedWork W2007826443 @default.
• W2022698551 hasRelatedWork W2020954148 @default.
• W2022698551 hasRelatedWork W2022698551 @default.
• W2022698551 hasRelatedWork W2059446717 @default.
• W2022698551 hasRelatedWork W2071557794 @default.
• W2022698551 hasRelatedWork W2090578102 @default.
• W2022698551 hasRelatedWork W2156592917 @default.
• W2022698551 isParatext "false" @default.
• W2022698551 isRetracted "false" @default.
• W2022698551 magId "2022698551" @default.
• W2022698551 workType "article" @default.