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• W2022700542 abstract "The ability of the analog 2′-fluoro-2′-deoxycytidine triphosphate (dCflTP) to be used as a substrate in the reactions catalyzed by Xenopus laevis oocytes DNA polymerase α and AMV reverse transcriptase has been studied. The apparent Km values for dCTP and dCflTP, using activated DNA as templates, were 0.6 μM and 7 mM with DNA polymerase α and 0.14 μM and 7 μM with AMV reverse transcriptase, respectively. As observed with dCTP, aphidicolin was a noncompetitive inhibitor in the DNA polymerase α-catalyzed DNA synthesis; the Ki values were about 2 μM for both substrates. dCflTP can also be incorporated into DNA synthetized by other eukaryotic DNA polymerases and by reverse transcriptase with RNA as a template, both in the presence or absence of (dT)12 primer." @default.
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• W2022700542 date "1985-03-01" @default.
• W2022700542 modified "2023-10-18" @default.
• W2022700542 title "2′-Fluoro-2′-deoxycytidine triphosphate as a substrate for RNA- and DNA-dependent DNA polymerases" @default.
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• W2022700542 doi "https://doi.org/10.1016/0167-4781(85)90051-x" @default.
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