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- W2022707517 abstract "The last several years have witnessed an explosion in the understanding and use of novel, versatile trans-acting elements. TALEs, CRISPR/Cas, and sRNAs can be easily fashioned to bind any specific sequence of DNA (TALEs, CRISPR/Cas) or RNA (sRNAs) because of the simple rules governing their interactions with nucleic acids. This unique property enables these tools to repress the expression of genes at the transcriptional or post-transcriptional levels, respectively, without prior manipulation of cis-acting and/or chromosomal target DNA sequences. These tools are now being harnessed by synthetic biologists, particularly those in the eukaryotic community, for genome-wide regulation, editing, or epigenetic studies. Here we discuss the exciting opportunities for using TALEs, CRISPR/Cas, and sRNAs as synthetic trans-acting regulators in prokaryotes." @default.
- W2022707517 created "2016-06-24" @default.
- W2022707517 creator A5037200355 @default.
- W2022707517 creator A5074579994 @default.
- W2022707517 creator A5083691379 @default.
- W2022707517 date "2014-10-01" @default.
- W2022707517 modified "2023-09-27" @default.
- W2022707517 title "Application of TALEs, CRISPR/Cas and sRNAs as trans-acting regulators in prokaryotes" @default.
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- W2022707517 doi "https://doi.org/10.1016/j.copbio.2014.02.010" @default.
- W2022707517 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4162867" @default.
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