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• W2022707976 abstract "Abstract Objective: To examine the expression of interleukin‐1 (IL‐1) and IL‐1 receptor antagonist (IL‐1ra) in the human endometrium in the follicular and luteal phases. Methods: The concentrations of IL‐1α and IL‐1β in the culture supernatants of endometrial cells were determined by enzyme‐linked immunosorbent assay. Transcription of the IL‐1ra gene in the endometrium was investigated by reverse polymerase chain reaction (PCR). Human endometrium was immunohistochemically stained using a monoclonal antibody specific to IL‐1ra. Results: The concentrations of IL‐1α and IL‐1β in the culture supernatants were 11 and 55 pg/ml, respectively, in the follicular phase, and 10 and 40 pg/ml, respectively, in the luteal phase. The concentration of IL‐1ra was 465 pg/ml in the follicular phase and 1710 pg/ml in the luteal phase. Densitometric analysis of the reverse PCR products showed that the expression of IL‐1ra mRNA was increased in endometrial cells in the luteal phase. Immunohistochemical staining revealed that epithelial cells were the main source of IL‐1ra in human endometrium. Conclusions: Human endometrial cells produce IL‐1 (mainly IL‐1β) and IL‐1ra. The level of IL‐1ra production in human endometrial epithelial cells was greater in the luteal phase than in the follicular phase due to the increased transcription of the IL‐1ra gene." @default.
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• W2022707976 date "1995-06-01" @default.
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• W2022707976 title "Interleukin-1 receptor antagonist expression in epithelial cells of human endometrium" @default.
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• W2022707976 doi "https://doi.org/10.1016/0020-7292(95)02366-k" @default.
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