Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022719473> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2022719473 endingPage "766" @default.
- W2022719473 startingPage "753" @default.
- W2022719473 abstract "1. The sulfhydryl reagent N-ethylmaleimide (NEM) was shown to inactivate the low affinity lysine transporter in human erythrocytes (system y+) without affecting the high affinity transporter (system y+L). 2. Pre-treatment of the cells with NEM reduced the rate of entry of L-[14C]lysine (1 microM) by approximately 50% (maximum effect). 3. NEM (0.2 mM) inhibited the NEM-sensitive component of the flux with mono-exponential kinetics. The inactivation rate constant (k, +/- S.E.M.) was 0.53 +/- 0.027 min-1 (25 degrees C). The substrate did not protect against inactivation. 4. Lysine self-inhibition experiments revealed two transport systems in untreated cells (half-saturation constants Km; +/- S.E.M.), 12.0 +/- 1.7 microM and 109 +/- 15.6 microM) and only one high affinity system in NEM-treated cells (Km 9.5 +/- 0.67 microM), indicating that NEM inactivates system y+. 5. The NEM-insensitive L-[14C]lysine influx (system y+L) was inhibited with high affinity by unlabelled neutral amino acids. The inhibition constant for L-leucine in sodium medium (Ki +/- S.E.M.) was 10.7 +/- 0.72 microM (37 degrees C). The system was also strongly inhibited by L-methionine, L-glutamine and with less affinity by L-phenylalanine and L-serine. N-methyl-L-leucine, L-proline and 2-amino-2-norbornane-carboxylic acid, a bicyclic analogue of leucine, did not exert a significant effect. 6. Lysine transport through system y+L occurred at the same rate in Na+, K+ or Li+ medium and the binding of lysine to the transporter was unaffected by Na+ replacement. 7. The interaction of system y+L with neutral amino acids was dependent on the cation present in the medium. The inhibition constant for leucine and glutamine increased approximately 90- and 60-fold respectively when Na+ was replaced by K+. Li+ was shown to be a very good substitute for Na+." @default.
- W2022719473 created "2016-06-24" @default.
- W2022719473 creator A5002645877 @default.
- W2022719473 creator A5025172898 @default.
- W2022719473 creator A5027737171 @default.
- W2022719473 date "1993-08-01" @default.
- W2022719473 modified "2023-10-06" @default.
- W2022719473 title "N-ethylmaleimide discriminates between two lysine transport systems in human erythrocytes." @default.
- W2022719473 cites W1495783055 @default.
- W2022719473 cites W1497464730 @default.
- W2022719473 cites W1524832496 @default.
- W2022719473 cites W1554256515 @default.
- W2022719473 cites W1556094526 @default.
- W2022719473 cites W1607466257 @default.
- W2022719473 cites W1969339813 @default.
- W2022719473 cites W1996096481 @default.
- W2022719473 cites W2020972755 @default.
- W2022719473 cites W2032299952 @default.
- W2022719473 cites W2046507378 @default.
- W2022719473 cites W2052099900 @default.
- W2022719473 cites W2060355646 @default.
- W2022719473 cites W2068183494 @default.
- W2022719473 cites W2069453488 @default.
- W2022719473 cites W2213680011 @default.
- W2022719473 cites W2439656324 @default.
- W2022719473 doi "https://doi.org/10.1113/jphysiol.1993.sp019799" @default.
- W2022719473 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1143854" @default.
- W2022719473 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8254535" @default.
- W2022719473 hasPublicationYear "1993" @default.
- W2022719473 type Work @default.
- W2022719473 sameAs 2022719473 @default.
- W2022719473 citedByCount "96" @default.
- W2022719473 countsByYear W20227194732012 @default.
- W2022719473 countsByYear W20227194732013 @default.
- W2022719473 countsByYear W20227194732015 @default.
- W2022719473 countsByYear W20227194732016 @default.
- W2022719473 countsByYear W20227194732019 @default.
- W2022719473 countsByYear W20227194732020 @default.
- W2022719473 countsByYear W20227194732021 @default.
- W2022719473 countsByYear W20227194732022 @default.
- W2022719473 crossrefType "journal-article" @default.
- W2022719473 hasAuthorship W2022719473A5002645877 @default.
- W2022719473 hasAuthorship W2022719473A5025172898 @default.
- W2022719473 hasAuthorship W2022719473A5027737171 @default.
- W2022719473 hasBestOaLocation W20227194732 @default.
- W2022719473 hasConcept C121332964 @default.
- W2022719473 hasConcept C148898269 @default.
- W2022719473 hasConcept C185592680 @default.
- W2022719473 hasConcept C2776016237 @default.
- W2022719473 hasConcept C2776580952 @default.
- W2022719473 hasConcept C515207424 @default.
- W2022719473 hasConcept C55493867 @default.
- W2022719473 hasConcept C62520636 @default.
- W2022719473 hasConcept C71240020 @default.
- W2022719473 hasConcept C93391505 @default.
- W2022719473 hasConceptScore W2022719473C121332964 @default.
- W2022719473 hasConceptScore W2022719473C148898269 @default.
- W2022719473 hasConceptScore W2022719473C185592680 @default.
- W2022719473 hasConceptScore W2022719473C2776016237 @default.
- W2022719473 hasConceptScore W2022719473C2776580952 @default.
- W2022719473 hasConceptScore W2022719473C515207424 @default.
- W2022719473 hasConceptScore W2022719473C55493867 @default.
- W2022719473 hasConceptScore W2022719473C62520636 @default.
- W2022719473 hasConceptScore W2022719473C71240020 @default.
- W2022719473 hasConceptScore W2022719473C93391505 @default.
- W2022719473 hasIssue "1" @default.
- W2022719473 hasLocation W20227194731 @default.
- W2022719473 hasLocation W20227194732 @default.
- W2022719473 hasLocation W20227194733 @default.
- W2022719473 hasOpenAccess W2022719473 @default.
- W2022719473 hasPrimaryLocation W20227194731 @default.
- W2022719473 hasRelatedWork W1506744214 @default.
- W2022719473 hasRelatedWork W1780521428 @default.
- W2022719473 hasRelatedWork W1975808600 @default.
- W2022719473 hasRelatedWork W1993795649 @default.
- W2022719473 hasRelatedWork W1997946388 @default.
- W2022719473 hasRelatedWork W2006281803 @default.
- W2022719473 hasRelatedWork W2044427462 @default.
- W2022719473 hasRelatedWork W2044628742 @default.
- W2022719473 hasRelatedWork W2047307026 @default.
- W2022719473 hasRelatedWork W2081940762 @default.
- W2022719473 hasVolume "468" @default.
- W2022719473 isParatext "false" @default.
- W2022719473 isRetracted "false" @default.
- W2022719473 magId "2022719473" @default.
- W2022719473 workType "article" @default.