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- W2022720289 abstract "Abstract The collision‐induced dissociation (CID) fragmentation reactions of a variety of deprotonated peptides containing proline have been studied in detail using MS 2 and MS 3 experiments, deuterium labelling and accurate mass measurements when necessary. The [MHCO 2 ] − (a 2 ) ion derived from H‐Pro‐Xxx‐OH dipeptides shows an unusual fragmentation involving loss of C 2 H 4 ; this fragmentation reaction is not observed for larger peptides. The primary fragmentation reactions of deprotonated tripeptides with an N‐terminal proline are formation of a 3 and y 1 ions. When proline is in the central position of tripeptides, a 3 , y 2 and y 1 ions are the primary fragmentation products of [MH] − , while when the proline is in the C‐terminal position, a 3 and y 1 ions are the major primary products. In the latter case, the a 3 ion fragments primarily to the ″b 2 ion; further evidence is presented that the ″b 2 ions have a deprotonated oxazolone structure. Larger deprotonated peptides having at least two amino acid residues N‐terminal to proline show a distinct preference for cleavage of the amide bond N‐terminal to proline to form, mainly, the appropriate y ion. This proline effect is compared and contrasted with the similar proline effect observed in the fragmentation of protonated peptides containing proline. Copyright © 2005 John Wiley & Sons, Ltd." @default.
- W2022720289 created "2016-06-24" @default.
- W2022720289 creator A5014175941 @default.
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- W2022720289 date "2005-07-22" @default.
- W2022720289 modified "2023-10-05" @default.
- W2022720289 title "Fragmentation reactions of deprotonated peptides containing proline. The proline effect" @default.
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- W2022720289 doi "https://doi.org/10.1002/jms.891" @default.
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