FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022741694> ?p ?o ?g. }

• W2022741694 endingPage "454" @default.
• W2022741694 startingPage "449" @default.
• W2022741694 abstract "In spite of the great interest to studies of the biological roles of chromium, as well as the toxic influence of Cr(VI)-species on living organisms, the molecular mechanisms of chromate bioremediation remain vague. A reductive pathway resulting in formation of less toxic Cr(III)-species is suggested to be the most important among possible mechanisms for chromate biodetoxification. The yeast l-lactate:cytochrome c-oxidoreductase (flavocytochrome b(2), FC b(2)) has absolute specificity for l-lactate, yet is non-selective with respect to its electron acceptor. These properties allow us to consider the enzyme as a potential candidate for chromate reduction by living cells in the presence of l-lactate. A recombinant strain of thermotolerant, methylotrophic yeast Hansenula polymorpha with sixfold increased FC b(2) enzyme activity (up to 3μmolmin(-1)mg(-1) protein in cell-free extract) compared to the parental strain was used for approval our suggestion. The recombinant cells, stored in dried state, as well as living yeast cells were tested for chromate-reducing activity in vitro in the presence of l-lactate (as an electron donor for chromate reduction) and different low molecular weight, redox-active mediators facilitating electron transfer from the reduced form of the enzyme to chromate (as a final electron acceptor): dichlorophenolindophenol (DCPIP), Methylene blue, Meldola blue, and Nile blue. It was shown that the highest chromate-reducing activity of the cells was achieved in the presence of DCPIP. The ability of chromate to catch electrons from the reduced flavocytochrome b(2) was confirmed using purified enzyme immobilized on the surface of a platinum electrode. The increasing concentration of Cr(VI) resulted in a decrease of enzyme-mediated current generated on the electrode during l-lactate oxidation. The shift and drop in amplitude of the peak in the cyclic voltammogram are indicative of Cr(VI)-dependent competition between reaction of chromate with reduced FC b(2) and direct electron transfer from the enzyme to the electrode surface. The application of the chromate-reducing ability of FC b(2)-over-producing recombinant cells of H. polymorpha toward chromate bioremediation and the construction of cells-based biosensor for chromate monitoring in the environment are discussed." @default.
• W2022741694 created "2016-06-24" @default.
• W2022741694 creator A5002105031 @default.
• W2022741694 creator A5050711996 @default.
• W2022741694 creator A5080331561 @default.
• W2022741694 creator A5081326534 @default.
• W2022741694 creator A5086994362 @default.
• W2022741694 date "2011-04-01" @default.
• W2022741694 modified "2023-09-25" @default.
• W2022741694 title "Chromate-reducing activity of Hansenula polymorpha recombinant cells over-producing flavocytochrome b2" @default.
• W2022741694 cites W1000655315 @default.
• W2022741694 cites W1507851645 @default.
• W2022741694 cites W1549921238 @default.
• W2022741694 cites W1954384501 @default.
• W2022741694 cites W1997839256 @default.
• W2022741694 cites W2004675398 @default.
• W2022741694 cites W2010744779 @default.
• W2022741694 cites W2013710006 @default.
• W2022741694 cites W2029367870 @default.
• W2022741694 cites W2030575147 @default.
• W2022741694 cites W2032891574 @default.
• W2022741694 cites W2035781101 @default.
• W2022741694 cites W2048024663 @default.
• W2022741694 cites W2054979333 @default.
• W2022741694 cites W2057843813 @default.
• W2022741694 cites W2059606814 @default.
• W2022741694 cites W2090304986 @default.
• W2022741694 cites W2091208087 @default.
• W2022741694 cites W2107280368 @default.
• W2022741694 cites W2110644516 @default.
• W2022741694 cites W2110822551 @default.
• W2022741694 cites W2111298094 @default.
• W2022741694 cites W2155339842 @default.
• W2022741694 cites W2158084036 @default.
• W2022741694 cites W2230688914 @default.
• W2022741694 cites W2329362233 @default.
• W2022741694 cites W2406615638 @default.
• W2022741694 cites W4240238777 @default.
• W2022741694 doi "https://doi.org/10.1016/j.chemosphere.2010.12.078" @default.
• W2022741694 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21315405" @default.
• W2022741694 hasPublicationYear "2011" @default.
• W2022741694 type Work @default.
• W2022741694 sameAs 2022741694 @default.
• W2022741694 citedByCount "13" @default.
• W2022741694 countsByYear W20227416942012 @default.
• W2022741694 countsByYear W20227416942013 @default.
• W2022741694 countsByYear W20227416942014 @default.
• W2022741694 countsByYear W20227416942015 @default.
• W2022741694 countsByYear W20227416942016 @default.
• W2022741694 countsByYear W20227416942019 @default.
• W2022741694 countsByYear W20227416942020 @default.
• W2022741694 countsByYear W20227416942021 @default.
• W2022741694 countsByYear W20227416942022 @default.
• W2022741694 crossrefType "journal-article" @default.
• W2022741694 hasAuthorship W2022741694A5002105031 @default.
• W2022741694 hasAuthorship W2022741694A5050711996 @default.
• W2022741694 hasAuthorship W2022741694A5080331561 @default.
• W2022741694 hasAuthorship W2022741694A5081326534 @default.
• W2022741694 hasAuthorship W2022741694A5086994362 @default.
• W2022741694 hasConcept C123669783 @default.
• W2022741694 hasConcept C13965031 @default.
• W2022741694 hasConcept C161790260 @default.
• W2022741694 hasConcept C178790620 @default.
• W2022741694 hasConcept C179104552 @default.
• W2022741694 hasConcept C181199279 @default.
• W2022741694 hasConcept C185592680 @default.
• W2022741694 hasConcept C205688572 @default.
• W2022741694 hasConcept C2124996 @default.
• W2022741694 hasConcept C2778148179 @default.
• W2022741694 hasConcept C2779191827 @default.
• W2022741694 hasConcept C2779222958 @default.
• W2022741694 hasConcept C2900893 @default.
• W2022741694 hasConcept C511782168 @default.
• W2022741694 hasConcept C523546767 @default.
• W2022741694 hasConcept C54355233 @default.
• W2022741694 hasConcept C55493867 @default.
• W2022741694 hasConcept C55904794 @default.
• W2022741694 hasConcept C65165184 @default.
• W2022741694 hasConcept C75473681 @default.
• W2022741694 hasConcept C86803240 @default.
• W2022741694 hasConceptScore W2022741694C123669783 @default.
• W2022741694 hasConceptScore W2022741694C13965031 @default.
• W2022741694 hasConceptScore W2022741694C161790260 @default.
• W2022741694 hasConceptScore W2022741694C178790620 @default.
• W2022741694 hasConceptScore W2022741694C179104552 @default.
• W2022741694 hasConceptScore W2022741694C181199279 @default.
• W2022741694 hasConceptScore W2022741694C185592680 @default.
• W2022741694 hasConceptScore W2022741694C205688572 @default.
• W2022741694 hasConceptScore W2022741694C2124996 @default.
• W2022741694 hasConceptScore W2022741694C2778148179 @default.
• W2022741694 hasConceptScore W2022741694C2779191827 @default.
• W2022741694 hasConceptScore W2022741694C2779222958 @default.
• W2022741694 hasConceptScore W2022741694C2900893 @default.
• W2022741694 hasConceptScore W2022741694C511782168 @default.
• W2022741694 hasConceptScore W2022741694C523546767 @default.
• W2022741694 hasConceptScore W2022741694C54355233 @default.
• W2022741694 hasConceptScore W2022741694C55493867 @default.
• W2022741694 hasConceptScore W2022741694C55904794 @default.

• next