FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022742288> ?p ?o ?g. }

• W2022742288 endingPage "4250" @default.
• W2022742288 startingPage "4237" @default.
• W2022742288 abstract "ABSTRACT A recombinant chimeric bovine/human parainfluenza type 3 virus (rB/HPIV3) vector expressing the respiratory syncytial virus (RSV) fusion F glycoprotein previously exhibited disappointing levels of RSV F immunogenicity and genetic stability in children (D. Bernstein et al., Pediatr. Infect. Dis. J. 31:109–114, 2012; C.-F. Yang et al., Vaccine 31:2822–2827, 2013). To investigate parameters that might affect vaccine performance and stability, we constructed and characterized rB/HPIV3 viruses expressing RSV F from the first (pre-N), second (N-P), third (P-M), and sixth (HN-L) genome positions. There was a 30- to 69-fold gradient in RSV F expression from the first to the sixth position. The inserts moderately attenuated vector replication in vitro and in the upper and lower respiratory tracts of hamsters: this was not influenced by the level of RSV F expression and syncytium formation. Surprisingly, inserts in the second, third, and sixth positions conferred increased temperature sensitivity: this was greatest for the third position and was the most attenuating in vivo . Each rB/HPIV3 vector induced a high titer of neutralizing antibodies in hamsters against RSV and HPIV3. Protection against RSV challenge was greater for position 2 than for position 6. Evaluation of insert stability suggested that RSV F is under selective pressure to be silenced during vector replication in vivo , but this was not exacerbated by a high level of RSV F expression and generally involved a small percentage of recovered vector. Vector passaged in vitro accumulated mutations in the HN open reading frame, causing a dramatic increase in plaque size that may have implications for vaccine production and immunogenicity. IMPORTANCE The research findings presented here will be instrumental for improving the design of a bivalent pediatric vaccine for respiratory syncytial virus and parainfluenza virus type 3, two major causes of severe respiratory tract infection in infants and young children. Moreover, this knowledge has general application to the development and clinical evaluation of other mononegavirus vectors and vaccines." @default.
• W2022742288 created "2016-06-24" @default.
• W2022742288 creator A5006369912 @default.
• W2022742288 creator A5015724534 @default.
• W2022742288 creator A5017357927 @default.
• W2022742288 creator A5027582911 @default.
• W2022742288 creator A5034229509 @default.
• W2022742288 creator A5034896027 @default.
• W2022742288 creator A5057228985 @default.
• W2022742288 creator A5071637505 @default.
• W2022742288 creator A5087096564 @default.
• W2022742288 date "2014-04-15" @default.
• W2022742288 modified "2023-10-18" @default.
• W2022742288 title "Chimeric Bovine/Human Parainfluenza Virus Type 3 Expressing Respiratory Syncytial Virus (RSV) F Glycoprotein: Effect of Insert Position on Expression, Replication, Immunogenicity, Stability, and Protection against RSV Infection" @default.
• W2022742288 cites W1530867604 @default.
• W2022742288 cites W1578183550 @default.
• W2022742288 cites W1605220975 @default.
• W2022742288 cites W1929167949 @default.
• W2022742288 cites W1973101424 @default.
• W2022742288 cites W1986189079 @default.
• W2022742288 cites W1989959574 @default.
• W2022742288 cites W1992597557 @default.
• W2022742288 cites W1996436367 @default.
• W2022742288 cites W1998423649 @default.
• W2022742288 cites W2002974329 @default.
• W2022742288 cites W2006931456 @default.
• W2022742288 cites W2007496089 @default.
• W2022742288 cites W2009078253 @default.
• W2022742288 cites W2009239426 @default.
• W2022742288 cites W2013391421 @default.
• W2022742288 cites W2014309691 @default.
• W2022742288 cites W2015929165 @default.
• W2022742288 cites W2016017622 @default.
• W2022742288 cites W2024624869 @default.
• W2022742288 cites W2026190929 @default.
• W2022742288 cites W2026944037 @default.
• W2022742288 cites W2027234508 @default.
• W2022742288 cites W2027729255 @default.
• W2022742288 cites W2031994163 @default.
• W2022742288 cites W2034196477 @default.
• W2022742288 cites W2035768087 @default.
• W2022742288 cites W2044117423 @default.
• W2022742288 cites W2055735404 @default.
• W2022742288 cites W2068160897 @default.
• W2022742288 cites W2071441855 @default.
• W2022742288 cites W2084746895 @default.
• W2022742288 cites W2102010453 @default.
• W2022742288 cites W2106953210 @default.
• W2022742288 cites W2109387251 @default.
• W2022742288 cites W2117847105 @default.
• W2022742288 cites W2118461307 @default.
• W2022742288 cites W2120949068 @default.
• W2022742288 cites W2123817481 @default.
• W2022742288 cites W2125526369 @default.
• W2022742288 cites W2132629607 @default.
• W2022742288 cites W2133963352 @default.
• W2022742288 cites W2134354003 @default.
• W2022742288 cites W2139677262 @default.
• W2022742288 cites W2143018999 @default.
• W2022742288 cites W2144516190 @default.
• W2022742288 cites W2145083936 @default.
• W2022742288 cites W2158794464 @default.
• W2022742288 cites W2159779332 @default.
• W2022742288 cites W2333994510 @default.
• W2022742288 cites W2128480412 @default.
• W2022742288 doi "https://doi.org/10.1128/jvi.03481-13" @default.
• W2022742288 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3993740" @default.
• W2022742288 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24478424" @default.
• W2022742288 hasPublicationYear "2014" @default.
• W2022742288 type Work @default.
• W2022742288 sameAs 2022742288 @default.
• W2022742288 citedByCount "26" @default.
• W2022742288 countsByYear W20227422882015 @default.
• W2022742288 countsByYear W20227422882016 @default.
• W2022742288 countsByYear W20227422882017 @default.
• W2022742288 countsByYear W20227422882018 @default.
• W2022742288 countsByYear W20227422882019 @default.
• W2022742288 countsByYear W20227422882020 @default.
• W2022742288 countsByYear W20227422882021 @default.
• W2022742288 countsByYear W20227422882022 @default.
• W2022742288 countsByYear W20227422882023 @default.
• W2022742288 crossrefType "journal-article" @default.
• W2022742288 hasAuthorship W2022742288A5006369912 @default.
• W2022742288 hasAuthorship W2022742288A5015724534 @default.
• W2022742288 hasAuthorship W2022742288A5017357927 @default.
• W2022742288 hasAuthorship W2022742288A5027582911 @default.
• W2022742288 hasAuthorship W2022742288A5034229509 @default.
• W2022742288 hasAuthorship W2022742288A5034896027 @default.
• W2022742288 hasAuthorship W2022742288A5057228985 @default.
• W2022742288 hasAuthorship W2022742288A5071637505 @default.
• W2022742288 hasAuthorship W2022742288A5087096564 @default.
• W2022742288 hasBestOaLocation W20227422881 @default.
• W2022742288 hasConcept C104317684 @default.
• W2022742288 hasConcept C108625454 @default.
• W2022742288 hasConcept C127413603 @default.
• W2022742288 hasConcept C140704245 @default.
• W2022742288 hasConcept C153911025 @default.
• W2022742288 hasConcept C159047783 @default.

• next