FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022752037> ?p ?o ?g. }

• W2022752037 endingPage "1722" @default.
• W2022752037 startingPage "1712" @default.
• W2022752037 abstract "The type II thioesterase EntH is a hotdog fold protein required for optimal nonribosomal biosynthesis of enterobactin in Escherichia coli. Its proposed proofreading activity in the biosynthesis is confirmed by its efficient restoration of enterobactin synthesis blocked in vitro by analogs of the cognate precursor 2,3-dihydroxybenzoate. Steady-state kinetic studies show that EntH recognizes the phosphopantetheine group and the pattern of hydroxylation in the aryl moiety of its thioester substrates. Remarkably, it is able to distinguish aberrant intermediates from the normal one in the enterobactin assembly line by demonstrating at least 10-fold higher catalytic efficiency toward thioesters derived from aberrant aryl precursors without a para-hydroxyl group, such as salicylate. By structural comparison and site-directed mutagenesis, the thioesterase is found to possess an active site closely resembling that of the 4-hydroxybenzoyl-CoA thioesterase from Arthrobacter sp. strain SU and to involve an acidic residue (glutamate-63) as the catalytic base or nucleophile like all other hotdog thioesterases. In addition, the EntH specificities toward the substrate hydroxylation pattern are found to depend on the active-site histidine-54, threonine-64, serine-67, and methionine-68 with the selectivity significantly reduced or even reversed when they are individually replaced by alanine. These residues are likely responsible for differential interaction of the enzyme with the substrates which leads to distinction between the normal and aberrant precursors in the enterobactin assembly line. These results show that the type II thioesterase evolves its distinctive ability to recognize the aberrant intermediates from the versatile catalytic platform of hotdog proteins and suggests an active search mechanism for type II thioesterases in nonribosomal peptide synthesis." @default.
• W2022752037 created "2016-06-24" @default.
• W2022752037 creator A5048665195 @default.
• W2022752037 creator A5068354292 @default.
• W2022752037 creator A5085656519 @default.
• W2022752037 creator A5089341631 @default.
• W2022752037 date "2009-02-04" @default.
• W2022752037 modified "2023-10-09" @default.
• W2022752037 title "Preferential Hydrolysis of Aberrant Intermediates by the Type II Thioesterase in <i>Escherichia coli</i> Nonribosomal Enterobactin Synthesis: Substrate Specificities and Mutagenic Studies on the Active-Site Residues" @default.
• W2022752037 cites W1573058404 @default.
• W2022752037 cites W1855537617 @default.
• W2022752037 cites W1935793071 @default.
• W2022752037 cites W1967759748 @default.
• W2022752037 cites W1969883879 @default.
• W2022752037 cites W1971511760 @default.
• W2022752037 cites W1974935234 @default.
• W2022752037 cites W1982793579 @default.
• W2022752037 cites W1983889089 @default.
• W2022752037 cites W1997589081 @default.
• W2022752037 cites W2018990299 @default.
• W2022752037 cites W2020848561 @default.
• W2022752037 cites W2022103709 @default.
• W2022752037 cites W2023229596 @default.
• W2022752037 cites W2044697909 @default.
• W2022752037 cites W2047504670 @default.
• W2022752037 cites W2050859221 @default.
• W2022752037 cites W2060275098 @default.
• W2022752037 cites W2073775906 @default.
• W2022752037 cites W2075947519 @default.
• W2022752037 cites W2076533036 @default.
• W2022752037 cites W2095717895 @default.
• W2022752037 cites W2098442675 @default.
• W2022752037 cites W2101295502 @default.
• W2022752037 cites W2102286080 @default.
• W2022752037 cites W2104291840 @default.
• W2022752037 cites W2107214348 @default.
• W2022752037 cites W2110397834 @default.
• W2022752037 cites W2111704552 @default.
• W2022752037 cites W2116774744 @default.
• W2022752037 cites W2119151987 @default.
• W2022752037 cites W2119366843 @default.
• W2022752037 cites W2121805080 @default.
• W2022752037 cites W2124254997 @default.
• W2022752037 cites W2128022667 @default.
• W2022752037 cites W2132034045 @default.
• W2022752037 cites W2132169354 @default.
• W2022752037 cites W2143956454 @default.
• W2022752037 cites W2144304328 @default.
• W2022752037 cites W2150781124 @default.
• W2022752037 cites W2159983165 @default.
• W2022752037 cites W2403297264 @default.
• W2022752037 doi "https://doi.org/10.1021/bi802165x" @default.
• W2022752037 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19193103" @default.
• W2022752037 hasPublicationYear "2009" @default.
• W2022752037 type Work @default.
• W2022752037 sameAs 2022752037 @default.
• W2022752037 citedByCount "30" @default.
• W2022752037 countsByYear W20227520372012 @default.
• W2022752037 countsByYear W20227520372013 @default.
• W2022752037 countsByYear W20227520372014 @default.
• W2022752037 countsByYear W20227520372017 @default.
• W2022752037 countsByYear W20227520372019 @default.
• W2022752037 countsByYear W20227520372020 @default.
• W2022752037 countsByYear W20227520372021 @default.
• W2022752037 countsByYear W20227520372022 @default.
• W2022752037 crossrefType "journal-article" @default.
• W2022752037 hasAuthorship W2022752037A5048665195 @default.
• W2022752037 hasAuthorship W2022752037A5068354292 @default.
• W2022752037 hasAuthorship W2022752037A5085656519 @default.
• W2022752037 hasAuthorship W2022752037A5089341631 @default.
• W2022752037 hasConcept C104317684 @default.
• W2022752037 hasConcept C181199279 @default.
• W2022752037 hasConcept C185592680 @default.
• W2022752037 hasConcept C2776414213 @default.
• W2022752037 hasConcept C2777379556 @default.
• W2022752037 hasConcept C2777547085 @default.
• W2022752037 hasConcept C2780622759 @default.
• W2022752037 hasConcept C2781109383 @default.
• W2022752037 hasConcept C41183919 @default.
• W2022752037 hasConcept C547475151 @default.
• W2022752037 hasConcept C553450214 @default.
• W2022752037 hasConcept C55493867 @default.
• W2022752037 hasConcept C71240020 @default.
• W2022752037 hasConceptScore W2022752037C104317684 @default.
• W2022752037 hasConceptScore W2022752037C181199279 @default.
• W2022752037 hasConceptScore W2022752037C185592680 @default.
• W2022752037 hasConceptScore W2022752037C2776414213 @default.
• W2022752037 hasConceptScore W2022752037C2777379556 @default.
• W2022752037 hasConceptScore W2022752037C2777547085 @default.
• W2022752037 hasConceptScore W2022752037C2780622759 @default.
• W2022752037 hasConceptScore W2022752037C2781109383 @default.
• W2022752037 hasConceptScore W2022752037C41183919 @default.
• W2022752037 hasConceptScore W2022752037C547475151 @default.
• W2022752037 hasConceptScore W2022752037C553450214 @default.
• W2022752037 hasConceptScore W2022752037C55493867 @default.
• W2022752037 hasConceptScore W2022752037C71240020 @default.
• W2022752037 hasIssue "8" @default.
• W2022752037 hasLocation W20227520371 @default.

• next