FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022752631> ?p ?o ?g. }

• W2022752631 endingPage "94" @default.
• W2022752631 startingPage "77" @default.
• W2022752631 abstract "This study is an in vitro part of the ongoing biomarker studies with population from a polluted region of Northern Bohemia and coke-oven workers from Czech and Slovak Republics. The aim of this study is to compare DNA adduct forming ability of chemical compound classes from both the urban and coke-oven extractable organic mass (EOM) of airborne particles. The crude extracts were fractionated into seven fractions by acid-base partitioning and silica gel column chromatography. In in vitro acellular assays we used calf thymus DNA (CT DNA) with oxidative (+S9) and reductive activation mediated by xanthine oxidase (+XO) under anaerobic conditions. Both the butanol and nuclease P1 versions of 32P-postlabeling for detection of bulky aromatic and/or hydrophobic adducts were used. The results showed that the spectra of major DNA adducts resulting from both the in vitro assays are within the fractions similar for both the urban and coke-oven samples. The highest DNA adduct levels with S9-activation were detected for the neutral aromatic fraction, followed by slightly polar and acidic fractions for both samples. With XO-mediated metabolism, the highest DNA adduct levels were detected for both the acidic fractions. Assuming additivity of compound activities, then the acidic fraction, which in the urban sample comprises a major portion of EOM mass (28%), may contain the greatest activity in both in vitro assays (39 and 69%, +S9 and +XO, respectively). In contrast, the aromatic fraction constituting only 8% of total urban EOM mass may account for comparable activity (34%) with organic acids. The highest DNA adduct forming activity of the coke-oven sample accounts for the aromatic fraction (82 and 63%, +S9 and +XO, respectively) that also contains the greatest portion of the total EOM (48%). To characterize some of the specific DNA adducts formed, we coupled TLC on 20x20 cm plates with HPLC analysis of 32P-postlabeled adducts. In both S9-treated samples of the aromatic fraction, we tentatively identified DNA adducts presumably diolepoxide-derived from: 9-hydroxy-benzo[a]pyrene (9-OH-B[a]P), benzo[a]pyrene-r-7,t-8-dihydrodiol-t-9,10-epoxide[+/-] (anti-BPDE), benzo[b,j,k]fluoranthenes (B[b]F, B[j]F, B[k]F), chrysene (CHRY), benz[a]-anthracene (B[a]A) and indeno[cd]pyrene (I[cd]P). These DNA adducts accounted for about 57% of total DNA adducts detected in both S9-treated samples of the aromatic fraction. DNA adducts of XO-treated samples were sensitive to nuclease P1 and HPLC profiles of the major adducts were markedly different from the major adducts of S9-treated samples. However, the combination of TLC and HPLC did not confirm the presence of DNA adducts derived from 1-nitropyrene (1 NP), 9-nitroanthracene (9 NA) and 3-nitrofluoranthene (3 NF) that were detected by GC-MS in the slightly polar fraction. We concluded that the chemical fractionation procedure facilitates the assessing of DNA adduct forming ability of different chemical compound classes. However, based on the results obtained with the whole extracts, it does not fulfil a task of the actual contribution of individual fractions within the activity of the whole extracts. Our results are the first in detecting of DNA adducts derived from urban air and coke-oven particulate matter." @default.
• W2022752631 created "2016-06-24" @default.
• W2022752631 creator A5007320411 @default.
• W2022752631 creator A5010394510 @default.
• W2022752631 creator A5030909315 @default.
• W2022752631 creator A5060242942 @default.
• W2022752631 creator A5066489892 @default.
• W2022752631 creator A5069213753 @default.
• W2022752631 creator A5080765239 @default.
• W2022752631 date "1998-05-01" @default.
• W2022752631 modified "2023-10-09" @default.
• W2022752631 title "Genotoxicity of coke-oven and urban air particulate matter in in vitro acellular assays coupled with -postlabeling and HPLC analysis of DNA adducts" @default.
• W2022752631 cites W1966467594 @default.
• W2022752631 cites W1966935428 @default.
• W2022752631 cites W1973789403 @default.
• W2022752631 cites W1979207335 @default.
• W2022752631 cites W1980023481 @default.
• W2022752631 cites W1982257809 @default.
• W2022752631 cites W1982411709 @default.
• W2022752631 cites W1984295174 @default.
• W2022752631 cites W1986162235 @default.
• W2022752631 cites W1986817703 @default.
• W2022752631 cites W1990410045 @default.
• W2022752631 cites W1991130089 @default.
• W2022752631 cites W1992705957 @default.
• W2022752631 cites W1994615418 @default.
• W2022752631 cites W1998233225 @default.
• W2022752631 cites W1999273921 @default.
• W2022752631 cites W2003630740 @default.
• W2022752631 cites W2005013799 @default.
• W2022752631 cites W2010341864 @default.
• W2022752631 cites W2010399478 @default.
• W2022752631 cites W2016662286 @default.
• W2022752631 cites W2017299876 @default.
• W2022752631 cites W2020550028 @default.
• W2022752631 cites W2025143557 @default.
• W2022752631 cites W2041366581 @default.
• W2022752631 cites W2041744128 @default.
• W2022752631 cites W2045367449 @default.
• W2022752631 cites W2048996333 @default.
• W2022752631 cites W2049160413 @default.
• W2022752631 cites W2050255635 @default.
• W2022752631 cites W2055206192 @default.
• W2022752631 cites W2061187594 @default.
• W2022752631 cites W2062360746 @default.
• W2022752631 cites W2071336475 @default.
• W2022752631 cites W2078962308 @default.
• W2022752631 cites W2088482033 @default.
• W2022752631 cites W2094303451 @default.
• W2022752631 cites W2113001570 @default.
• W2022752631 cites W2124778417 @default.
• W2022752631 cites W2128442121 @default.
• W2022752631 cites W2141390733 @default.
• W2022752631 cites W4249361881 @default.
• W2022752631 doi "https://doi.org/10.1016/s1383-5718(98)00040-0" @default.
• W2022752631 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9630530" @default.
• W2022752631 hasPublicationYear "1998" @default.
• W2022752631 type Work @default.
• W2022752631 sameAs 2022752631 @default.
• W2022752631 citedByCount "22" @default.
• W2022752631 countsByYear W20227526312013 @default.
• W2022752631 countsByYear W20227526312018 @default.
• W2022752631 countsByYear W20227526312020 @default.
• W2022752631 crossrefType "journal-article" @default.
• W2022752631 hasAuthorship W2022752631A5007320411 @default.
• W2022752631 hasAuthorship W2022752631A5010394510 @default.
• W2022752631 hasAuthorship W2022752631A5030909315 @default.
• W2022752631 hasAuthorship W2022752631A5060242942 @default.
• W2022752631 hasAuthorship W2022752631A5066489892 @default.
• W2022752631 hasAuthorship W2022752631A5069213753 @default.
• W2022752631 hasAuthorship W2022752631A5080765239 @default.
• W2022752631 hasConcept C107872376 @default.
• W2022752631 hasConcept C108204754 @default.
• W2022752631 hasConcept C114246631 @default.
• W2022752631 hasConcept C144024400 @default.
• W2022752631 hasConcept C149923435 @default.
• W2022752631 hasConcept C178790620 @default.
• W2022752631 hasConcept C185592680 @default.
• W2022752631 hasConcept C2778959862 @default.
• W2022752631 hasConcept C2908647359 @default.
• W2022752631 hasConcept C29730261 @default.
• W2022752631 hasConcept C43617362 @default.
• W2022752631 hasConcept C55493867 @default.
• W2022752631 hasConceptScore W2022752631C107872376 @default.
• W2022752631 hasConceptScore W2022752631C108204754 @default.
• W2022752631 hasConceptScore W2022752631C114246631 @default.
• W2022752631 hasConceptScore W2022752631C144024400 @default.
• W2022752631 hasConceptScore W2022752631C149923435 @default.
• W2022752631 hasConceptScore W2022752631C178790620 @default.
• W2022752631 hasConceptScore W2022752631C185592680 @default.
• W2022752631 hasConceptScore W2022752631C2778959862 @default.
• W2022752631 hasConceptScore W2022752631C2908647359 @default.
• W2022752631 hasConceptScore W2022752631C29730261 @default.
• W2022752631 hasConceptScore W2022752631C43617362 @default.
• W2022752631 hasConceptScore W2022752631C55493867 @default.
• W2022752631 hasIssue "1-3" @default.
• W2022752631 hasLocation W20227526311 @default.
• W2022752631 hasLocation W20227526312 @default.

• next