FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022754867> ?p ?o ?g. }

• W2022754867 endingPage "622" @default.
• W2022754867 startingPage "618" @default.
• W2022754867 abstract "Two conserved histidine residues (His-101 and His-271) appear to be essential components in the active site of the enzyme xylose (glucose) isomerase (EC 5.3.1.5). These amino acid residues were targeted for mutagenesis on the basis of sequence homology among xylose isomerases isolated from Escherichia coli, Bacillus subtilis, Ampullariella sp. strain 3876, and Streptomyces violaceus-niger. Each residue was selectively replaced by site-directed mutagenesis and shown to be essential for activity. No measurable activity was observed for any mutations replacing either His-101 or His-271. Circular dichroism measurements revealed no significant change in the overall conformation of the mutant enzymes, and all formed dimers similar to the wild-type enzyme. Mutations at His-271 could be distinguished from those at His-101, since the former resulted in a thermolabile protein whereas no significant change in heat stability was observed for the latter. Based upon these results and structural data recently reported, we speculate that His-101 is the catalytic base mediating the reaction. Replacement of His-271 may render the enzyme thermolabile, since this residue appears to be a ligand for one of the metal ions in the active site of the enzyme." @default.
• W2022754867 created "2016-06-24" @default.
• W2022754867 creator A5037038213 @default.
• W2022754867 creator A5049836136 @default.
• W2022754867 creator A5091771844 @default.
• W2022754867 date "1990-01-01" @default.
• W2022754867 modified "2023-10-10" @default.
• W2022754867 title "Identification of essential histidine residues in the active site of Escherichia coli xylose (glucose) isomerase." @default.
• W2022754867 cites W1500330894 @default.
• W2022754867 cites W1505350514 @default.
• W2022754867 cites W1528166121 @default.
• W2022754867 cites W1599864785 @default.
• W2022754867 cites W1601687245 @default.
• W2022754867 cites W1663588906 @default.
• W2022754867 cites W1964507128 @default.
• W2022754867 cites W1966750327 @default.
• W2022754867 cites W1977750999 @default.
• W2022754867 cites W2007376635 @default.
• W2022754867 cites W2024470778 @default.
• W2022754867 cites W2027231406 @default.
• W2022754867 cites W2032029099 @default.
• W2022754867 cites W2047456345 @default.
• W2022754867 cites W2057379115 @default.
• W2022754867 cites W2059727401 @default.
• W2022754867 cites W2080033436 @default.
• W2022754867 cites W2096249575 @default.
• W2022754867 cites W2155638944 @default.
• W2022754867 cites W2745627398 @default.
• W2022754867 doi "https://doi.org/10.1073/pnas.87.2.618" @default.
• W2022754867 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/53316" @default.
• W2022754867 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2405386" @default.
• W2022754867 hasPublicationYear "1990" @default.
• W2022754867 type Work @default.
• W2022754867 sameAs 2022754867 @default.
• W2022754867 citedByCount "36" @default.
• W2022754867 countsByYear W20227548672012 @default.
• W2022754867 countsByYear W20227548672015 @default.
• W2022754867 countsByYear W20227548672017 @default.
• W2022754867 countsByYear W20227548672020 @default.
• W2022754867 countsByYear W20227548672021 @default.
• W2022754867 crossrefType "journal-article" @default.
• W2022754867 hasAuthorship W2022754867A5037038213 @default.
• W2022754867 hasAuthorship W2022754867A5049836136 @default.
• W2022754867 hasAuthorship W2022754867A5091771844 @default.
• W2022754867 hasBestOaLocation W20227548671 @default.
• W2022754867 hasConcept C103408237 @default.
• W2022754867 hasConcept C104317684 @default.
• W2022754867 hasConcept C143065580 @default.
• W2022754867 hasConcept C181199279 @default.
• W2022754867 hasConcept C185592680 @default.
• W2022754867 hasConcept C2777272437 @default.
• W2022754867 hasConcept C2778460671 @default.
• W2022754867 hasConcept C2779155592 @default.
• W2022754867 hasConcept C2779277513 @default.
• W2022754867 hasConcept C2781264208 @default.
• W2022754867 hasConcept C41183919 @default.
• W2022754867 hasConcept C523546767 @default.
• W2022754867 hasConcept C54355233 @default.
• W2022754867 hasConcept C547475151 @default.
• W2022754867 hasConcept C55493867 @default.
• W2022754867 hasConcept C71240020 @default.
• W2022754867 hasConcept C86803240 @default.
• W2022754867 hasConceptScore W2022754867C103408237 @default.
• W2022754867 hasConceptScore W2022754867C104317684 @default.
• W2022754867 hasConceptScore W2022754867C143065580 @default.
• W2022754867 hasConceptScore W2022754867C181199279 @default.
• W2022754867 hasConceptScore W2022754867C185592680 @default.
• W2022754867 hasConceptScore W2022754867C2777272437 @default.
• W2022754867 hasConceptScore W2022754867C2778460671 @default.
• W2022754867 hasConceptScore W2022754867C2779155592 @default.
• W2022754867 hasConceptScore W2022754867C2779277513 @default.
• W2022754867 hasConceptScore W2022754867C2781264208 @default.
• W2022754867 hasConceptScore W2022754867C41183919 @default.
• W2022754867 hasConceptScore W2022754867C523546767 @default.
• W2022754867 hasConceptScore W2022754867C54355233 @default.
• W2022754867 hasConceptScore W2022754867C547475151 @default.
• W2022754867 hasConceptScore W2022754867C55493867 @default.
• W2022754867 hasConceptScore W2022754867C71240020 @default.
• W2022754867 hasConceptScore W2022754867C86803240 @default.
• W2022754867 hasIssue "2" @default.
• W2022754867 hasLocation W20227548671 @default.
• W2022754867 hasLocation W20227548672 @default.
• W2022754867 hasLocation W20227548673 @default.
• W2022754867 hasLocation W20227548674 @default.
• W2022754867 hasOpenAccess W2022754867 @default.
• W2022754867 hasPrimaryLocation W20227548671 @default.
• W2022754867 hasRelatedWork W1978979732 @default.
• W2022754867 hasRelatedWork W1996035747 @default.
• W2022754867 hasRelatedWork W2032029099 @default.
• W2022754867 hasRelatedWork W2047154665 @default.
• W2022754867 hasRelatedWork W2065228811 @default.
• W2022754867 hasRelatedWork W2093535643 @default.
• W2022754867 hasRelatedWork W2096249575 @default.
• W2022754867 hasRelatedWork W2486194337 @default.
• W2022754867 hasRelatedWork W2501381229 @default.
• W2022754867 hasRelatedWork W4253666163 @default.
• W2022754867 hasVolume "87" @default.
• W2022754867 isParatext "false" @default.

• next