Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022757461> ?p ?o ?g. }
- W2022757461 endingPage "2796" @default.
- W2022757461 startingPage "2786" @default.
- W2022757461 abstract "Cholesterol is the major sterol component of all mammalian plasma membranes. Recent studies have shown that cholesterol inhibits both bacterial (KirBac1.1 and KirBac3.1) and eukaryotic (Kir2.1) inward rectifier K+ (Kir) channels. Lipid-sterol interactions are not enantioselective, and the enantiomer of cholesterol (ent-cholesterol) does not inhibit Kir channel activity, suggesting that inhibition results from direct enantiospecific binding to the channel, and not indirect effects of changes to the bilayer. Furthermore, conservation of the effect of cholesterol among prokaryotic and eukaryotic Kir channels suggests an evolutionary conserved cholesterol-binding pocket, which we aimed to identify. Computational experiments were performed by docking cholesterol to the atomic structures of Kir2.2 (PDB: 3SPI) and KirBac1.1 (PDB: 2WLL) using Autodock 4.2. Poses were assessed to ensure biologically relevant orientation and then clustered according to location and orientation. The stability of cholesterol in each of these poses was then confirmed by molecular dynamics simulations. Finally, mutation of key residues (S95H and I171L) in this putative binding pocket found within the transmembrane domain of Kir2.1 channels were shown to lead to a loss of inhibition by cholesterol. Together, these data provide support for this location as a biologically relevant pocket." @default.
- W2022757461 created "2016-06-24" @default.
- W2022757461 creator A5000857231 @default.
- W2022757461 creator A5055257287 @default.
- W2022757461 creator A5063073652 @default.
- W2022757461 creator A5088002796 @default.
- W2022757461 date "2014-12-01" @default.
- W2022757461 modified "2023-10-03" @default.
- W2022757461 title "Identification of a Cholesterol-Binding Pocket in Inward Rectifier K + (Kir) Channels" @default.
- W2022757461 cites W1518056784 @default.
- W2022757461 cites W1526205344 @default.
- W2022757461 cites W1968786790 @default.
- W2022757461 cites W1973724314 @default.
- W2022757461 cites W1976907717 @default.
- W2022757461 cites W1979709047 @default.
- W2022757461 cites W1979732884 @default.
- W2022757461 cites W1983482324 @default.
- W2022757461 cites W1986076204 @default.
- W2022757461 cites W1990926659 @default.
- W2022757461 cites W1996338088 @default.
- W2022757461 cites W1996484675 @default.
- W2022757461 cites W2007061409 @default.
- W2022757461 cites W2016555254 @default.
- W2022757461 cites W2016730940 @default.
- W2022757461 cites W2020185913 @default.
- W2022757461 cites W2021520922 @default.
- W2022757461 cites W2022543156 @default.
- W2022757461 cites W2030310238 @default.
- W2022757461 cites W2033206973 @default.
- W2022757461 cites W2037714333 @default.
- W2022757461 cites W2038827150 @default.
- W2022757461 cites W2039084338 @default.
- W2022757461 cites W2042559329 @default.
- W2022757461 cites W2044995190 @default.
- W2022757461 cites W2045740138 @default.
- W2022757461 cites W2049093635 @default.
- W2022757461 cites W2049230063 @default.
- W2022757461 cites W2051221402 @default.
- W2022757461 cites W2052501554 @default.
- W2022757461 cites W2055527954 @default.
- W2022757461 cites W2056283223 @default.
- W2022757461 cites W2058730349 @default.
- W2022757461 cites W2066075286 @default.
- W2022757461 cites W2069519194 @default.
- W2022757461 cites W2072994787 @default.
- W2022757461 cites W2087612672 @default.
- W2022757461 cites W2092880628 @default.
- W2022757461 cites W2095553393 @default.
- W2022757461 cites W2098879446 @default.
- W2022757461 cites W2103770142 @default.
- W2022757461 cites W2125041118 @default.
- W2022757461 cites W2129300000 @default.
- W2022757461 cites W2129955267 @default.
- W2022757461 cites W2130745677 @default.
- W2022757461 cites W2134762386 @default.
- W2022757461 cites W2137015675 @default.
- W2022757461 cites W2140698310 @default.
- W2022757461 cites W2144124959 @default.
- W2022757461 cites W2147645103 @default.
- W2022757461 cites W2150981663 @default.
- W2022757461 cites W2151694767 @default.
- W2022757461 cites W2161022768 @default.
- W2022757461 cites W2401231806 @default.
- W2022757461 cites W2418687657 @default.
- W2022757461 doi "https://doi.org/10.1016/j.bpj.2014.10.066" @default.
- W2022757461 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4269788" @default.
- W2022757461 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25517146" @default.
- W2022757461 hasPublicationYear "2014" @default.
- W2022757461 type Work @default.
- W2022757461 sameAs 2022757461 @default.
- W2022757461 citedByCount "27" @default.
- W2022757461 countsByYear W20227574612016 @default.
- W2022757461 countsByYear W20227574612017 @default.
- W2022757461 countsByYear W20227574612018 @default.
- W2022757461 countsByYear W20227574612019 @default.
- W2022757461 countsByYear W20227574612020 @default.
- W2022757461 countsByYear W20227574612021 @default.
- W2022757461 countsByYear W20227574612022 @default.
- W2022757461 countsByYear W20227574612023 @default.
- W2022757461 crossrefType "journal-article" @default.
- W2022757461 hasAuthorship W2022757461A5000857231 @default.
- W2022757461 hasAuthorship W2022757461A5055257287 @default.
- W2022757461 hasAuthorship W2022757461A5063073652 @default.
- W2022757461 hasAuthorship W2022757461A5088002796 @default.
- W2022757461 hasBestOaLocation W20227574611 @default.
- W2022757461 hasConcept C104317684 @default.
- W2022757461 hasConcept C118892022 @default.
- W2022757461 hasConcept C159110408 @default.
- W2022757461 hasConcept C170493617 @default.
- W2022757461 hasConcept C185592680 @default.
- W2022757461 hasConcept C2775905019 @default.
- W2022757461 hasConcept C2778163477 @default.
- W2022757461 hasConcept C2778718757 @default.
- W2022757461 hasConcept C2780152424 @default.
- W2022757461 hasConcept C41685203 @default.
- W2022757461 hasConcept C50254741 @default.
- W2022757461 hasConcept C55493867 @default.
- W2022757461 hasConcept C65556437 @default.