Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022757632> ?p ?o ?g. }
- W2022757632 endingPage "553" @default.
- W2022757632 startingPage "550" @default.
- W2022757632 abstract "We developed a new method of glucose sensing using an inactive form of glucose oxidase from Aspergillus niger. Glucose oxidase was rendered inactive by removal of the FAD cofactor. The resulting apo-glucose oxidase still binds glucose as observed from a decrease in its intrinsic tryptophan fluorescence. 8-Anilino-1-naphthalenesulfonic acid (ANS) was found to bind spontaneously to apo-glucose oxidase as seen from an enhancement of the ANS fluorescence. The steady state intensity of the bound ANS decreased 25% upon binding of glucose, and the mean lifetime of the bound ANS decreased about 40%. These spectral changes occurred with a midpoint from 10 to 20 mM glucose, which is comparable to the K(D) of holo-glucose oxidase. These results suggest that apo-glucose oxidase can be used as a reversible nonconsuming sensor for glucose." @default.
- W2022757632 created "2016-06-24" @default.
- W2022757632 creator A5038720075 @default.
- W2022757632 creator A5060740762 @default.
- W2022757632 creator A5074798370 @default.
- W2022757632 creator A5087375961 @default.
- W2022757632 date "1999-09-01" @default.
- W2022757632 modified "2023-10-17" @default.
- W2022757632 title "The Fluorescence Emission of the Apo-glucose Oxidase from Aspergillus niger as Probe to Estimate Glucose Concentrations" @default.
- W2022757632 cites W1489223607 @default.
- W2022757632 cites W1973953570 @default.
- W2022757632 cites W2002996516 @default.
- W2022757632 cites W2003881870 @default.
- W2022757632 cites W2005336425 @default.
- W2022757632 cites W2006297603 @default.
- W2022757632 cites W2020702811 @default.
- W2022757632 cites W2026013887 @default.
- W2022757632 cites W2035655231 @default.
- W2022757632 cites W2043199616 @default.
- W2022757632 cites W2046153401 @default.
- W2022757632 cites W2058182041 @default.
- W2022757632 cites W2059081738 @default.
- W2022757632 cites W2070272023 @default.
- W2022757632 cites W2071330110 @default.
- W2022757632 cites W2074372414 @default.
- W2022757632 cites W2079557951 @default.
- W2022757632 cites W2081334813 @default.
- W2022757632 cites W2089521574 @default.
- W2022757632 cites W2133574204 @default.
- W2022757632 cites W2142807591 @default.
- W2022757632 cites W2149482687 @default.
- W2022757632 cites W2419935176 @default.
- W2022757632 cites W2464915368 @default.
- W2022757632 cites W2769264260 @default.
- W2022757632 cites W4243473954 @default.
- W2022757632 doi "https://doi.org/10.1006/bbrc.1999.1330" @default.
- W2022757632 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6943336" @default.
- W2022757632 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10491329" @default.
- W2022757632 hasPublicationYear "1999" @default.
- W2022757632 type Work @default.
- W2022757632 sameAs 2022757632 @default.
- W2022757632 citedByCount "70" @default.
- W2022757632 countsByYear W20227576322012 @default.
- W2022757632 countsByYear W20227576322013 @default.
- W2022757632 countsByYear W20227576322014 @default.
- W2022757632 countsByYear W20227576322015 @default.
- W2022757632 countsByYear W20227576322016 @default.
- W2022757632 countsByYear W20227576322017 @default.
- W2022757632 countsByYear W20227576322018 @default.
- W2022757632 countsByYear W20227576322020 @default.
- W2022757632 countsByYear W20227576322021 @default.
- W2022757632 countsByYear W20227576322022 @default.
- W2022757632 crossrefType "journal-article" @default.
- W2022757632 hasAuthorship W2022757632A5038720075 @default.
- W2022757632 hasAuthorship W2022757632A5060740762 @default.
- W2022757632 hasAuthorship W2022757632A5074798370 @default.
- W2022757632 hasAuthorship W2022757632A5087375961 @default.
- W2022757632 hasBestOaLocation W20227576322 @default.
- W2022757632 hasConcept C121332964 @default.
- W2022757632 hasConcept C181199279 @default.
- W2022757632 hasConcept C185592680 @default.
- W2022757632 hasConcept C197957613 @default.
- W2022757632 hasConcept C2775960407 @default.
- W2022757632 hasConcept C2776706248 @default.
- W2022757632 hasConcept C2777197210 @default.
- W2022757632 hasConcept C38485361 @default.
- W2022757632 hasConcept C515207424 @default.
- W2022757632 hasConcept C55493867 @default.
- W2022757632 hasConcept C62520636 @default.
- W2022757632 hasConcept C91881484 @default.
- W2022757632 hasConceptScore W2022757632C121332964 @default.
- W2022757632 hasConceptScore W2022757632C181199279 @default.
- W2022757632 hasConceptScore W2022757632C185592680 @default.
- W2022757632 hasConceptScore W2022757632C197957613 @default.
- W2022757632 hasConceptScore W2022757632C2775960407 @default.
- W2022757632 hasConceptScore W2022757632C2776706248 @default.
- W2022757632 hasConceptScore W2022757632C2777197210 @default.
- W2022757632 hasConceptScore W2022757632C38485361 @default.
- W2022757632 hasConceptScore W2022757632C515207424 @default.
- W2022757632 hasConceptScore W2022757632C55493867 @default.
- W2022757632 hasConceptScore W2022757632C62520636 @default.
- W2022757632 hasConceptScore W2022757632C91881484 @default.
- W2022757632 hasIssue "2" @default.
- W2022757632 hasLocation W20227576321 @default.
- W2022757632 hasLocation W20227576322 @default.
- W2022757632 hasLocation W20227576323 @default.
- W2022757632 hasLocation W20227576324 @default.
- W2022757632 hasOpenAccess W2022757632 @default.
- W2022757632 hasPrimaryLocation W20227576321 @default.
- W2022757632 hasRelatedWork W1164537646 @default.
- W2022757632 hasRelatedWork W2000774291 @default.
- W2022757632 hasRelatedWork W2001387027 @default.
- W2022757632 hasRelatedWork W2030842578 @default.
- W2022757632 hasRelatedWork W2035731844 @default.
- W2022757632 hasRelatedWork W2080203695 @default.
- W2022757632 hasRelatedWork W2123102716 @default.
- W2022757632 hasRelatedWork W2387160967 @default.
- W2022757632 hasRelatedWork W3194873095 @default.